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A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats

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Abstract

Exercise has been demonstrated to enhance subsequent insulin-stimulated glucose uptake (GU) by predominantly type II (fast-twitch) muscle of old rats, but previous research has not evaluated exercise effects on GU by type I (slow-twitch) muscle from old rats. Accordingly, we studied male Fischer 344/Brown Norway rats (24 months old) and determined GU (0, 100, 200, and 5,000 μU/ml insulin) of isolated soleus (predominantly type I) and epitrochlearis (predominantly type II) muscles after one exercise session. Epitrochlearis (100, 200, and 5,000 μU/ml insulin) and soleus (100 and 200 μU/ml insulin) GU were greater at 3-h postexercise vs. age-matched sedentary controls. Insulin receptor tyrosine phosphorylation (Tyr1162/1163) was unaltered by exercise in either muscle. Akt phosphorylation (pAkt) was greater for exercised vs. sedentary rats in the epitrochlearis (Ser473 and Thr308 with 100 and 200 μU/ml, respectively) and soleus (Ser473 with 200 μU/ml). AS160 phosphorylation (pAS160) was greater for exercised vs. sedentary rats in the epitrochlearis (Thr642 with 100 μU/ml), but not the soleus. Exercised vs. sedentary rats did not differ for total protein abundance of insulin receptor, Akt, AS160, or GLUT4 in either muscle. These results demonstrate that both predominantly type I and type II muscles from old rats are susceptible to exercise-induced improvement in insulin-mediated GU by mechanisms that are independent of enhanced insulin receptor tyrosine phosphorylation or altered abundance of important signaling proteins or GLUT4. Exercise-induced elevation in pAkt, and possibly pAS160, may contribute to this effect in the epitrochlearis of old rats, but other mechanisms are likely important for the soleus.

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Acknowledgments

This research was supported by the National Institutes of Health Grant AG-010026.

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Authors and Affiliations

  1. Muscle Biology Laboratory, School of Kinesiology, University of Michigan, 401 Washtenaw Ave., Ann Arbor, MI, 48109-2214, USA

    Yuanyuan Xiao, Naveen Sharma, Edward B. Arias, Carlos M. Castorena & Gregory D. Cartee

  2. Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, 48109, USA

    Gregory D. Cartee

  3. Institute of Gerontology, University of Michigan, Ann Arbor, MI, 48109, USA

    Gregory D. Cartee

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  1. Yuanyuan Xiao
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  2. Naveen Sharma
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  5. Gregory D. Cartee
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Correspondence to Gregory D. Cartee.

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Xiao, Y., Sharma, N., Arias, E.B. et al. A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats. AGE 35, 573–582 (2013). https://doi.org/10.1007/s11357-012-9383-0

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  • DOI: https://doi.org/10.1007/s11357-012-9383-0

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