Abstract
The objective of this study was to investigate the quantitative characteristics of short tandem repeat (STR) variations deduced on the basis of the number of STRs that are beneficial for human survival. The longevity group included 60 nonagenarian subjects, and the control group included 250 reference adults (age, 20–50 years). Alleles of 15 Combined DNA Index System STR loci were determined using a commercial polymerase chain reaction kit. An STR with the highest frequency distribution in a population (control group) was considered as a conservative STR, and the number of core unit repeats of this STR allele was considered as the median repeat number in the STR locus (STRm). The absolute difference between the STRm and the number of core unit repeats of other STR alleles can be considered as the quantitative marker of variation for that particular STR allele (M value). The mean M values of CSF1TPO in the longevity group were significantly higher than those in the control group (P < 0.05). These findings appear to suggest that at least one of the STR loci may be associated with longevity. The M value of STR may be a new and high-efficacy genetic marker.
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References
Anselmi CV, Malovini A, Roncarati R, Novelli V, Villa F, Condorelli G, Bellazzi R, Puca AA (2009) Association of the FOXO3A locus with extreme longevity in a southern Italian centenarian study. Rejuvenation Res 12(2):95–104
Browner WS, Kahn AJ, Ziv E, Reiner AP, Oshima J, Cawthon RM, Hsueh WC, Cummings SR (2004) The genetics of human longevity. Am J Med 117(11):882–883
Budowle B, Sprecher CJ (2001) Concordance study on population database samples using the PowerPlex 16 kit and AmpFlSTR Profiler Plus kit and AmpFlSTR COfiler kit. J Forensic Sci 46(3):637–641
Buse EL, Putinier JC, Hong MM, Yap AE, Hartmann JM (2003) Performance evaluation of two multiplexes used in fluorescent short tandem repeat DNA analysis. J Forensic Sci 48(2):348–357
Butler JM (2006) Genetics and genomics of core short tandem repeat loci used in human identity testing. J Forensic Sci 51(2):253–265
Eckert KA, Hile SE (2009) Every microsatellite is different: intrinsic DNA features dictate mutagenesis of common microsatellites present in the human genome. Mol Carcinog 48(4):379–388
Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756
Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric repeat loci in four human population groups. Genomics 12:241–253
Fan H, Chu JY (2007) A brief review of short tandem repeat mutation. Genomics Proteomics Bioinformatics 5(1):7–14
Kim KI, Na JE, Kang SY, Cho YS, Choi DJ, Kim CH, Kim HS, Oh BH, Choi YH, Kwon IS, Park SC (2007) Impact of NAD(P)H oxidase p22 phox gene polymorphism on vascular aging in Korean centenarian and nonagenarian. Int J Cardiol 123(1):18–22
Laghi L, Bianchi P, Malesci A (2008) Differences and evolution of the methods for the assessment of microsatellite instability. Oncogene 27(49):6313–6321
Lim EJ, Lee HY, Sim JE, Yang WI, Shin KJ (2009) Genetic polymorphism and haplotype analysis of 4 tightly linked X-STR duos in Koreans. Croat Med J 50(3):305–312
Liu H, Wang B, Liu D, Cheng Y, Yu W, Liang X, An W (2007) Molecular genetic studies on relationships among longevity, diseases, and HLA-DRB1/DQB1 allelic polymorphism. Exp Aging Research 33:123–125
Moretti TR, Baumstark AL, Defenbaugh DA, Keys KM, Smerick JB, Budowle B (2001) Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. J Forensic Sci 46(3):647–660
Tan Q, Bellizzi D, Rose G, Garasto S, Franceschi C, Kruse T, Vaupel JW, De Benedictis G, Yashin AI (2002) The influences on human longevity by HUMTHO1.STR polymorphism (Tyrosine Hydroxylase gene). A relative risk approach. Mech Ageing Dev 123(10):1403–1410
Vijg J, Suh Y (2005) Genetics of longevity and aging. Annu Rev Med 56:193–212
Yamaya M, Nakayama K, Ebihara S, Hirai H, Higuchi S, Sasaki H (2003) Relationship between microsatellite polymorphism in the haem oxygenase-1 gene promoter and longevity of the normal Japanese population. J Med Genet 40(2):146–148
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Hui, L., Weijian, Y., Xuelian, D. et al. Variations in short tandem repeats deduced on the basis of the number of repeats and the relationship of these variations with longevity. AGE 33, 63–68 (2011). https://doi.org/10.1007/s11357-010-9160-x
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DOI: https://doi.org/10.1007/s11357-010-9160-x