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Molecular mechanisms of physiological change under acute total dissolved gas supersaturation stress in yellow catfish (Pelteobagrus fulvidraco)

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Abstract

During the dam discharging period, the strong aeration of high-speed water leads to the supersaturation of total dissolved gas (TDG) in the downstream water, which causes gas bubble disease (GBD) in fish and threatens their survival. TDG supersaturation has now become an ecological and environmental issue of global concern; however, the molecular mechanism underlying the physiological effect of TDG supersaturation on fish is poorly known. Here, we comprehensively investigated the effect of TDG supersaturation on Pelteobagrus fulvidraco at the histopathological, biochemical, transcriptomic, and metabolomic levels. After exposure to 116% TDG, P. fulvidraco exhibited classic GBD symptoms and pathological changes in gills. The level of superoxide dismutase was highly significantly decreased. Transcriptomic results revealed that heat shock proteins (HSPs) and a large number of genes involved in immunity were increased by TDG stress. A key environmental sensor PI3K/Akt/mTOR pathway was significantly stimulated for defence against stress. Integrated transcriptomic and metabolomic analyses revealed that key metabolites and genes were upregulated in the triacylglycerol synthesis pathway and that amino acid levels decreased, which might be associated with TDG supersaturation stress. The present study demonstrated that TDG supersaturation could cause severe physiological damage in fish. HSP genes, immune functions, and energy metabolic pathways were enhanced to counteract the adverse effects.

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References

  • Alarcon-Vila C, Pizzuto M, Pelegrín P (2019) Purinergic receptors and the inflammatory response mediated by lipids. Curr Opin Pharmacol 47:90–96

    CAS  Google Scholar 

  • Boone AN, Vijayan MM (2002) Constitutive heat shock protein (HSC70) expression in rainbow trout hepatocytes: effect of heat shock and heavy metal exposure. Comp Biochem Physiol C 132:223–233

    Google Scholar 

  • Brosnan IG, Welch DW, Jacobs SM (2016) Survival rates of out-migrating yearling Chinook salmon in the lower Columbia River and plume after exposure to gas-supersaturated water. J Aquat Anim Health 28:240–251

    CAS  Google Scholar 

  • Cao J, Li JL, Li DM, Tobin JF, Gimeno RE (2006) Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis. Proc Natl Acad Sci USA 103:19695–19700

    CAS  Google Scholar 

  • Cappello T, Brandão F, Guilherme S, Santos MA, Maisano M, Mauceri A, Canário J, Pacheco M, Pereira P (2016) Insights into the mechanisms underlying mercuryinduced oxidative stress in gills of wild fish (Liza aurata) combining 1H NMRmetabolomics and conventional biochemical assays. Sci Total Environ 548:13–24

    Google Scholar 

  • Chen YB, Peng QD, Liao WG (2009) The evolvement study on supersaturation of dissolved gas in the middle reaches of Yangtze River after the Three Gorges Project running. J Hydroecol 2:1–5

    Google Scholar 

  • Chen SC, Liu XQ, Jiang W, Li KF, Du J, Shen DZ, Gong Q (2012) Effects of total dissolved gas supersaturated water on lethality and catalase activity of Chinese sucker (Myxocyprinus asiaticus Bleeker). J Zhejiang Univ-Sci B 13:791–796

    Google Scholar 

  • Da Silva JLG, Passos DF, Bernardes VM, Leal DBR (2019) ATP and adenosine: role in the immunopathogenesis of rheumatoid arthritis. Immunol Lett 214:55–64

    Google Scholar 

  • De Freitas SC, Baldissera MD, Barroso DC, Val AL, Baldisserotto B (2020) Purine levels and purinergic signaling in plasma and spleen of Brycon amazonicus exposed to acute heat thermal stress: an attempt to regulate the immune response. J Therm Biol 89:102569

    Google Scholar 

  • Deane EE, Woo NYS (2010) Growth hormone attenuates branchial HSP70 expression in silver sea bream. Fish Physiol Biochem 36:135–140

    CAS  Google Scholar 

  • Dennis PB, Jaeschke A, Saitoh M, Fowler B, Kozma SC, Thomas G (2001) Mammalian TOR: a homeostatic ATP sensor. Science 294:1102–1105

    CAS  Google Scholar 

  • Dhabhar FS (2002) Stress-induced augmentation of immune function–the role of stress hormones, leukocyte trafficking, and cytokines. Brain Behav Immun 16:785–798

    CAS  Google Scholar 

  • Dhabhar FS (2008) Enhancing versus suppressive effects of stress on immune function: implications for immunoprotection versus immunopathology. Allergy Asthma Clin Immunol 4:2–11

    CAS  Google Scholar 

  • Dhabhar FS, Miller AH, McEwen BS, Spencer RL (1995) Effects of stress on immune cell distribution. Dynamics and hormonal mechanisms. J Immunol 154:5511–5527

    CAS  Google Scholar 

  • Ditlecadet D, Short CE, Driedzic WR (2011) Glycerol loss to water exceeds glycerol catabolism via glycerol kinase in freeze-resistant rainbow smelt (Osmerus mordax). American Journal of Physiology. Regul, Integr Comp Physiol 300:674–684

    Google Scholar 

  • Dorfman JR, Stefanova I, Yasutomo K, Germain RN (2000) CD4+ T cells survival is not directly linked to self-MHC-induced TCR signaling. Nat Immunol 1:329–335

    CAS  Google Scholar 

  • Duan JL, Yin J, Ren WK, Liu T, Cui ZJ, Huang XG, Wu L, Kim SW, Liu G, Wu X, Wu GY, Li TJ, Yin YL (2016) Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide. Amino Acids 48:53–64

    CAS  Google Scholar 

  • Evans DH, Piermarini PM, Choe KP (2005) The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85:97–177

    CAS  Google Scholar 

  • Fan ZQ, Deng YX, Yuan Q, Liu XQ, Shi XR, Feng CX, Yang Y, Xu LF (2020) Effect of total dissolved gas supersaturation on the tolerance of grass carp (Ctenopharyngodon idellus). Environ Sci Eur 32:55

    CAS  Google Scholar 

  • Fast MD, Johnson SC, Jones SRM (2007) Differential expression of the pro-inflammatory cytokines IL-1β-1, TNFα-1 and IL-8 in vaccinated pink (Oncorhynchus gorbuscha) and chum (Oncorhynchus keta) salmon juveniles. Fish Shellfish Immunol 22:403–407

    CAS  Google Scholar 

  • Feng JJ, Li R, Ma Q, Wang LL (2014) Experimental and field study on dissipation coefficient of supersaturated total dissolved gas. J Cent South Univ 21:1995–2003

    CAS  Google Scholar 

  • Gopal R, Birdsell D, Monroy FP (2011) Regulation of chemokine responses in intestinal epithelial cells by stress and Toxoplasma gondii infection. Parasite Immunol 33:12–24

    CAS  Google Scholar 

  • He Y, Zhao T, Lv WH, Tan XY, Song YF, Song CC, Zhong CC, Luo Z (2023) Interactive effects of dietary zinc and water temperature on growth performance, Zn transport, heat shock response and lipid metabolism in the livers of juvenile yellow catfish Pelteobagrus fulvidraco. Aquaculture 738795:562

    Google Scholar 

  • Huang J, Li Y, Zhe L, Kang YJ, Wang JF (2018) Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney. Fish Shellfish Immunol 82:32–40

    CAS  Google Scholar 

  • Jastrebski SF, Lamont SJ, Schmidt CJ (2017) Chicken hepatic response to chronic heat stress using integrated transcriptome and metabolome analysis. PLoS ONE 12:e0181900

    Google Scholar 

  • Ji CL, Li F, Wang Q, Zhao JM, Sun ZD, Wu HF (2016) An integrated proteomic and metabolomic study on the gender-specific responses of mussels Mytilus galloprovincialis, to tetrabromobisphenol A (TBBPA). Chemosphere 144:527–539

    CAS  Google Scholar 

  • Ji QF, Xue SD, Yuan Q, Yuan Y, Wang YM, Liang RF, Feng JJ, Li KF, Li R (2019) The tolerance characteristics of resident fish in the upper Yangtze river under varying gas supersaturation. Int J Environ Res Public Health 16:2021

    Google Scholar 

  • Jia SC, Li XY, Zheng SX, Wu GY (2017) Amino acids are major energy substrates for tissues of hybrid striped bass and zebrafish. Amino Acids 49:1–11

    Google Scholar 

  • Jin YX, Chen RJ, Liu WP, Fu ZW (2010) Effect of endocrine disrupting chemicals on the transcription of genes related to the innate immune system in the early developmental stage of zebrafish (Danio rerio). Fish Shellfish Immunol 28:854–861

    CAS  Google Scholar 

  • Josse C, Boelaert JR, Best-Belpomme M, Piette J (2001) Importance of post-transcriptional regulation of chemokine genes by oxidative stress. Biochemical Journal 360:321–333

    CAS  Google Scholar 

  • Kumar N, Gupta SK, Bhushan S, Singh NP (2019) Impacts of acute toxicity of arsenic (III) alone and with high temperature on stress biomarkers, immunological status and cellular metabolism in fish. Aquat Toxicol 214:105233

    CAS  Google Scholar 

  • Laiz-Carrión R, Viana IR, Cejas JR, Ruiz-Jarabo I, Jerez S, Martos JA, Eduardo AB, Mancera JM (2012) Influence of food deprivation and high stocking density on energetic metabolism and stress response in red porgy, Pagrus pagrus L. Aquacult Int 20:585–599

    Google Scholar 

  • Lermen CL, Vieira VL, Schetinger MR, Lappe R, Gioda CR, Crestani MR, Baldisserotto B, Morsch VM (2005) ATP, ADP and AMP dephosphorylation in membrane fractions of Rhamdia quelen exposed to different temperatures. Fish Physiol Biochem 31:295–301

    CAS  Google Scholar 

  • Li P, Mai K, Trushenski J, Wu G (2009) New developments in fish amino acid nutrition: towards functional and environmentally oriented aquafeeds. Amino Acids 37:43–53

    Google Scholar 

  • Li M, Gong S, Li Q, Yuan L, Meng F, Wang R (2016) Ammonia toxicity induces glutamine accumulation, oxidative stress and immunosuppression in juvenile yellow catfish Pelteobagrus fulvidraco. Comp Biochem Physiol C-Toxicol Pharmacol 183–184:1–6

    Google Scholar 

  • Li N, Fu CH, Zhang J, Liu XQ, Shi XT, Yang Y, Shi HR (2019) Hatching rate of Chinese sucker (Myxocyprinus asiaticus Bleeker) eggs exposed to total dissolved gas (TDG) supersaturation and the tolerance of juveniles to the interaction of TDG supersaturation and suspended sediment. Aquac Res 50:1876–1884

    Google Scholar 

  • Li J, Zhang GS, Yin DQ, Li Y, Zhang YR, Cheng JH, Zhang K, Ji J, Wang T, Jia YY, Yin SW (2021) Integrated application of multiomics strategies provides insights into the environmental hypoxia response in pelteobagrus fulvidraco muscle. Mol Cell Proteomics 21:100196

    Google Scholar 

  • Lin H, Decuypere E, Buyse J (2006) Acute heat stress induces oxidative stress in broiler chickens. Comp Biochem Physiol 144A:11–17

    CAS  Google Scholar 

  • Liu XQ, Li KF, Du J, Li J, Li R (2011) Growth rate, catalase and superoxide dismutase activities in rock carp (Procypris rabaudi Tchang) exposed to supersaturated total dissolved gas. J Zhejiang Univ-Sci B 12:909–914

    CAS  Google Scholar 

  • Liu XQ, Li KF, Jiang W, Wu S (2015) Biochemical responses and survival of rock carp (Procypris rabaudi) to total dissolved gas supersaturated water. Ichthyol Res 62:171–176

    Google Scholar 

  • Liu XQ, Li N, Feng CX, Fu CH, Gong Q, Lai JS, Jiang Z, Yang Y, Shi HR (2019) Lethal effect of total dissolved gas-supersaturated water with suspended sediment on river sturgeon (Acipenser dabryanus). Sci Rep 9:13373

    Google Scholar 

  • Lu J, Shi Y, Cai S, Feng J (2017) Metabolic responses of Haliotis diversicolor to Vibrio parahaemolyticus infection. Fish Shellfish Immunol 60:265–274

    CAS  Google Scholar 

  • Lutz D (2011) Gas supersaturation and gas bubble trauma in fish downstream from a Midwestern reservoir. Trans Am Fish Soc 5:423–436

    Google Scholar 

  • Ma KX, Chen GW, Liu DZ (2012) cDNA cloning of heat shock protein 90 gene and protein expression pattern in response to heavy metal exposure and thermal stress in planarian Dugesia japonica. Mol Biol Rep 39:7203–7210

    CAS  Google Scholar 

  • Mackay CR (2001) Chemokines: immunology’s high impact factors. Nat Immunol 2:95–101

    CAS  Google Scholar 

  • Mahmoud HK, Reda FM, Alagawany M, Farag MR (2021) The stress of abamectin toxicity reduced water quality, growth performance, immunity and antioxidant capacity of Oreochromis niloticus fish: modulatory role of Simmondsia chinensis extract as a dietary supplement. Aquaculture 534:736247

    CAS  Google Scholar 

  • Malik NS, Perez JL, Kunta M (2013) Changes in free amino acid levels in sour orange leaves in response to cold stress and during recovery from cold stress. J Food Agric Environ 11:1086–1088

    CAS  Google Scholar 

  • Mellado M, Rodriguez-Frade JM, Manes S, Martinez AC (2001) Chemokine signaling and functional responses: the role of receptor dimerization and TK pathway activation. Annu Rev Immunol 19:397–421

    CAS  Google Scholar 

  • Melvin SD, Lanctot CM, Doriean NJC, Bennett W, Carroll AR (2019) NMR-based lipidomics of fish from a metal (loid) contaminated wetland show differences consistent with effects on cellular membranes and energy storage. Sci Total Environ 654:284–291

    CAS  Google Scholar 

  • Ming JH, Xie J, Xu P, Liu WB, Ge XP, Liu B, He YJ, Cheng YF, Zhou QL, Pan LK (2010) Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Fish Shellfish Immunol 28:407–418

    CAS  Google Scholar 

  • Mu W, Wen H, Li J, He F (2013) Cloning and expression analysis of a HSP70 gene from Korean rockfish (Sebastes schlegeli). Fish Shellfish Immunol 35:1111–1121

    CAS  Google Scholar 

  • Müller WEG, Koziol C, Kurelec B, Dapper J, Batel R, Rinkevich B (1995) Combinatory effects of temperature stress and nonionic organic pollutants on stress protein (hsp70) gene expression in the fresh water sponge Ephydatia fluviatilis. Environ Toxicol Chem 14:1203–1208

    Google Scholar 

  • Pierron F, Bourret V, St-Cyr J, Campbell PGC, Bernatchez L, Couture P (2009) Transcriptional responses to environmental metal exposure in wild yellow perch (Perca flavescens) collected in lakes with differing metal levels (Cd, Cu, Ni). Ecotoxicology 18:620–631

    CAS  Google Scholar 

  • Polinski M (2014) Application of molecular methods for determining immune responses and disease status in Bluefin tuna. Dissertation, University of Tasmania

  • Powell EN, Kasschau M, Chen E, Koenig M, Pecon J (1982) Changes in the free amino acid pool during environmental stress in the gill tissue of the oyster, Crassostrea virginica. Comparative biochemistry and physiology. Part a, Mol Integr Physiol 71:591–598

    Google Scholar 

  • Qu L, Li R, Li J, Li KF, Deng Y (2011) Field observation of total dissolved gas supersaturation of high-dams. Sci China Ser e: Technol Sci 54:156–162

    Google Scholar 

  • Rabasa C, Dickson SL (2016) Impact of stress on metabolism and energy balance. Curr Opin Behav Sci 9:71–77

    Google Scholar 

  • Rio DC, Ares M, Hannon GJ, Nilsen TW (2010) Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc 6:5439

    Google Scholar 

  • Romphruk AV, Naruse TK, Romphruk A, Kawata H, Puapairoj C, Kulski JK, Leelayuwat C, Inoko H (2001) Diversity typing MICA and MICB alleles using PCR-SSP 793 of MICA (PERB11.1) and HLA haplotypes in Northeastern Thais. Tissue Antigens 58:83

    CAS  Google Scholar 

  • Sanghera KP, Mathalone N, Baigi R, Panov E, Wang D, Zhao X, Hsu H, Wang H, Tropepe V, Ward M, Boyd SR (2011) The PI3K/Akt/mTOR pathway mediates retinal progenitor cell survival under hypoxic and superoxide stress. Mol Cell Neurosci 47:145–153

    CAS  Google Scholar 

  • Shi KP, Dong SL, Zhou YG, Li Y, Gao QF, Sun DJ (2019) RNA-seq reveals temporal differences in the transcriptome response to acute heat stress in the Atlantic salmon (Salmo salar). Comp Biochem Physiol D-Genomics Proteomics 30:169–178

    CAS  Google Scholar 

  • Smiley JE, Drawbridge MA, Okihiro MS, Kaufmann RS (2011) Acute effects of gas supersaturation on juvenile cultured white seabass. Trans Am Fish Soc 140:1269–1276

    Google Scholar 

  • Song M, Zhao J, Wen HS, Li Y, Li JF, Li LM, Tao YX (2019) The impact of acute thermal stress on the metabolome of the black rockfish (Sebastes schlegelii). PLoS ONE 14:e0217133

    CAS  Google Scholar 

  • Stenberg S K (2016) Effects of gas supersaturation on migrating Atlantic salmon smolt (Salmo salar) in Evangervatnet. Dissertation, The University of Bergen

  • Wang YM, An RD, Li Y, Li KF (2017) Swimming performance of rock carp Procypris rabaudi and Prenant’s Schizothoracin Schizothorax prenanti acclimated to total dissolved gas supersaturated water. North Am J Fish Manag 37:1183–1190

    Google Scholar 

  • Wang YM, Li Y, An RD, Li KF (2018) Effects of total dissolved gas supersaturation on the swimming performance of two endemic fish species in the upper Yangtze River. Sci Rep 8:10063

    Google Scholar 

  • Wang M, Wu F, Xie S, Zhang L (2021) Acute hypoxia and reoxygenation: effect on oxidative stress and hypoxia signal transduction in the juvenile yellow catfish (Pelteobagrus fulvidraco). Aquaculture 531:735903

    CAS  Google Scholar 

  • Wang J, Wang M, Li B, Guo H, Zhu X, Zhang L (2022) The combined effect of acute hypoxic stress and feeding status on the metabolism of yellow catfish (Pelteobagrus fulvidraco). Aquaculture 2022:738605

    Google Scholar 

  • Wen B, Mei Z, Zeng CW, Liu SQ (2017) metaX: a flexible and comprehensive software for processing metabolomics data. BMC Bioinformatics 18:183

    Google Scholar 

  • Witt A, Stewart K, Hadjerioua B (2017) Predicting total dissolved gas travel time in hydropower reservoirs. J Environ Eng 143:06017011

    Google Scholar 

  • Wu GY (2010) Functional amino acids in growth, reproduction, and health. Adv Nutr 1:31–37

    CAS  Google Scholar 

  • Xue SD, Li KF, Liang RF, Lu C, Wang YM, Li Y, Feng JJ (2019a) In situ study on the impact of total dissolved gas supersaturation on endemic fish in the Upper Yangtze River. River Res Appl 35:1–9

    Google Scholar 

  • Xue SD, Wang YM, Liang RF, Li KF, Li R (2019b) Effects of total dissolved gas supersaturation in fish of different sizes and species. Int J Environ Res Public Health 16:2444

    CAS  Google Scholar 

  • Yue X, Liu B, Sun L, Tang B (2011) Cloning and characterization of a HSP70 gene from Asiatic hard clam Meretrix meretrix which is involved in the immune response against bacterial infection. Fish Shellfish Immunol 30:791–799

    CAS  Google Scholar 

  • Zhang Y, Foster R, Sun X, Yin Q, Li Y, Hanley G, Stuart C, Gan Y, Li C, Zhang Z, Yin D (2008) Restraint stress induces lymphocyte reduction through p53 and PI3K/NF-kappaB pathways. J Neuroimmunol 200:71–76

    CAS  Google Scholar 

  • Zhang MZ, Li M, Wang RX, Qian YX (2018) Effects of acute ammonia toxicity on oxidative stress, immune response and apoptosis of juvenile yellow catfish Pelteobagrus fulvidraco and the mitigation of exogenous taurine. Fish Shellfish Immunol 79:313–320

    CAS  Google Scholar 

  • Zhang M, Yin X, Li M, Wang R, Qian Y, Hong M (2020a) Effect of nitrite exposure on haematological status, oxidative stress, immune response and apoptosis in yellow catfish (Pelteobagrus fulvidraco). Comp Biochem Physiol C-Toxicol Pharmacol 238:108867

    CAS  Google Scholar 

  • Zhang W, Song Y, Chai TT, Liao GQ, Zhang L, Jia Q, Qian YZ, Qiu J (2020) Lipidomics perturbations in the brain of adult zebrafish (Danio rerio) after exposure to chiral ibuprofen. Sci Total Environ 713:136565

    CAS  Google Scholar 

  • Zhao LL, Sun JL, Liang J, Liu Q, Luo J, Li ZQ, Yan TM, Zhou J, Yang S (2020) Enhancing lipid metabolism and inducing antioxidant and immune responses to adapt to acute hypoxic stress in Schizothorax prenanti. Aquaculture 519:734933

    Google Scholar 

  • Zheng X, Fu D, Cheng J, Tang R, Chu M, Chu P, Wang T, Yin SW (2021) Effects of hypoxic stress and recovery on oxidative stress, apoptosis, and intestinal microorganisms in Pelteobagrus fulvidraco. Aquaculture 543:736945

    Google Scholar 

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Funding

This work was supported by grants from the Sichuan Science and Technology Program (2021YFYZ0015) and National Natural Science Foundation of China (52079117).

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  1. The Fishery Institute of the Sichuan Academy of Agricultural Sciences, Chengdu, 611730, China

    Yeyu Chen, Xiaoyun Wu, Jiansheng Lai, Boqin Yan & Quan Gong

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  1. Yeyu Chen
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Contributions

Yeyu Chen: writing—review and editing, writing—original draft, and conceptualization. Xiaoyun Wu: methodology and formal analysis. Jiansheng Lai: resources and formal analysis. Boqin Yan: methodology and validation. Quan Gong: resources, formal analysis, writing—review and editing, conceptualization, and funding acquisition.

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Correspondence to Quan Gong.

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The study protocol was approved by the Animal Care and Use Committee of the Fishery Institute of the Sichuan Academy of Agricultural Sciences (20211221002A) and was performed in compliance with the guidelines for the care and use of laboratory animals.

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Chen, Y., Wu, X., Lai, J. et al. Molecular mechanisms of physiological change under acute total dissolved gas supersaturation stress in yellow catfish (Pelteobagrus fulvidraco). Environ Sci Pollut Res 30, 97911–97924 (2023). https://doi.org/10.1007/s11356-023-29157-6

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