Skip to main content

Advertisement

SpringerLink
Log in

Effect of Fe3O4 nanoparticles on seed germination in tobacco

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

Many studies on the toxicity of nanoparticles (NPs) have reported different levels of toxicity for various types of NPs. This study aimed to examine the morpho-ultrastructural impact of iron oxide (Fe3O4) NPs on seed germination in tobacco (Nicotiana tabacum var. Turkish) using different sizes and concentrations of nanoparticles. Seeds were allowed to germinate in the presence of (Fe3O4) NPs of three different sizes (5, 10, and 20 nm) at three different concentrations 3, 10, and 30 mg/L for each size. Seeds were assessed using light and transmission microscopy. Radical lengths and seed germination rate were significantly affected (positively or negatively) in all NPs-treated seeds compared to control seeds. The radical lengths in 5 nm-treated seeds (30 mg/L concentration) and 10 nm-treated seeds (10 and 30 mg/L concentrations) were significantly shorter than control seeds. In contrast, the radical lengths in 10 nm-treated seeds (3 mg/L concentration) and 20 nm-treated seeds (10 mg/L concentration) were significantly longer than control seeds. Most NPs-treated seeds exhibited significant higher seed germination except for seeds treated with 5 nm NPs (3 mg/L concentration). Moreover, thick and thin micrographs of radicles and leaflets of 5 nm NPs-treated seeds (30 mg/L concentration) and 10 nm NPs (30 mg/L concentration) showed structural and ultrastructural deformation. Thus, these findings confirm that the toxicity and the bioaccumulation of (Fe3O4) NPs were size and concentration dependent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Availability of data and materials

All data generated or analyzed during this study are included in this published article (and its supplementary information files).

References

  • Acharya P, Jayaprakasha GK, Crosby KM, Jifon JL, Patil BS (2020) Nanoparticle-mediated seed priming improves germination, growth, yield, and quality of watermelons (Citrullus lanatus) at multi-locations in Texas. Sci Rep 10:5037

    Article  CAS  Google Scholar 

  • Alkhatib R, Alkhatib B, AL-Quraan N, Al-Eitan L, Muhaidat R (2016) Impact of exogenous caffeine on morphological, biochemical, and ultrastructural characteristics in Nicotiana tabacum var. Turkish. Biol Plant 60:706–714

    Article  CAS  Google Scholar 

  • Alkhatib R, Alkhatib B, Al-Eitan L, Abdo N, Tadros M, Bsoul E (2018) Physio-anatomical responses of tobacco under caffeine stress. Photosynthetica 56(4):1140–1146

    Article  CAS  Google Scholar 

  • Alkhatib R, Alkhatib B, Abdo N, Al-Eitan L, Creamer R (2019) Physio-biochemical and ultrastructural impact of (Fe3O4) nanoparticles on tobacco. BMC Plant Biol 19:253–265

    Article  Google Scholar 

  • Banerjee J, Kole C (2016) Plant nanotechnology: an overview on concepts, strategies, and tools. In: Kole C, Kumar D, Khodakovskaya M (eds) Plant Nanotechnology. Springer, Cham, pp 1–14

    Google Scholar 

  • Disfani MN, Mikhak A, Kassaee MZ, Maghari AE (2017) Effects of nano Fe/SiO2 fertilizers on germination and growth of barley and maize. Arch Agron Soil Sci 63:817–826

    Article  Google Scholar 

  • El-Temsah YS, Joner EJ (2012) Impact of Fe and Ag nanoparticles on seed germination and differences in bioavailability during exposure in aqueous suspension and soil. Environ Toxicol 27:42–49

    Article  CAS  Google Scholar 

  • Epstein MA, Holt SJ (1963) The localization by electron microscopy of HeLa cell surface enzymes splitting adenosine triphosphate. J Cell Biol 19:325–326

    Article  CAS  Google Scholar 

  • Hussain A, Rizwan M, Ali Q, Ali S (2019) Seed priming with silicon nanoparticles improved the biomass and yield while reduced the oxidative stress and cadmium concentration in wheat grains. Environ Sci Pollut Res 26:7579–7588

    Article  CAS  Google Scholar 

  • Iannone MF, Groppa MD, De Sousa ME, Van Raap MBF, Benavides MP (2016) Impact of magnetite iron oxide nanoparticles on wheat (Triticum aestivum L.) development: evaluation of oxidative damage. Environ Exp Bot 131:77–88

    Article  CAS  Google Scholar 

  • Khot LR, Sankaran S, Maja JM, Ehsani R, Schuster EW (2012) Applications of nanomaterials in agricultural production and crop protection: a review. Crop Prot 35:64–70

    Article  CAS  Google Scholar 

  • Kim JH, Lee Y, Kim EJ, Gu S, Sohn EJ, Seo YS, An HJ, Chang YS (2014) Exposure of iron nanoparticles to Arabidopsis thaliana enhances root elongation by triggering cell wall loosening. Environ Sci Technol 48:3477–3434

    Article  CAS  Google Scholar 

  • Konatea A, Wanga Y, Hec X, Adeela M, Zhang P, Mac Y, Dingc Y, Zhangc J, Yanga J, Kizitoa S, Ruia Y, Zhangc Z (2018) Comparative effects of nano and bulk-Fe3O4 on the growth of cucumber (Cucumis sativus). Ecotoxicol Environ Saf 165:547–554

    Article  Google Scholar 

  • Kornarzyński K, Sujak A, Czernel G, Wiącek D (2020) Effect of Fe3O4 nanoparticles on germination of seeds and concentration of elements in Helianthus annuus L. under constant magnetic field. Sci Rep 10:8068

    Article  Google Scholar 

  • Kumar V, Guleria P, Kumar V, Yadav SK (2013) Gold nanoparticle exposure induces growth and yield enhancement in Arabidopsis thaliana. Sci Total Environ 461:462–468

    Article  Google Scholar 

  • Kumari M, Mukherjee A, Chandrasekaran N (2009) Genotoxicity of silver nanoparticles in Allium cepa. Sci Total Environ 407:5243–5246

  • Lu C, Zhang C, Wen J, Wu G, Tao MX (2002) Research of the effect of nanometer materials on germination and growth enhancement of Glycine max and its mechanism. Soybean Sci 21:168–171

    CAS  Google Scholar 

  • Lv J, Christie P, Zhang S (2019) Uptake, translocation and transformation of metal-based nanoparticles in plants: recent advances and methodological challenges. Environ Sci Nano 6:41–59

    Article  CAS  Google Scholar 

  • Ma CX, Geisler-Lee J, Deng Y, Kolmakov A (2010) Interactions between engineered nanoparticles (ENPs) and plants: phytotoxicity, uptake and accumulation. Sci Total Environ 408:3053–3061

    Article  CAS  Google Scholar 

  • Ma CX, Gurung A, Deng Y (2013a) Phytotoxicity and uptake of nanoscale zero-valent iron (nZVI) by two plant species. Sci Total Environ 443:844–849

    Article  CAS  Google Scholar 

  • Ma CX, Chhikara S, Xing BS, Musante C, White JC, Dhankher OP (2013b) Physiological and molecular response of Arabidopsis thaliana (L.) to nanoparticle cerium and indium oxide exposure. ACS Sustain Chem Eng J 1:768–778

    Article  CAS  Google Scholar 

  • Mahakham W, Sarmah AK, Santi MS, Theerakulpisut P (2017) Nanopriming technology for enhancing germination and starch metabolism of aged rice seeds using phytosynthesized silver nanoparticles. Sci Rep 7:8263

    Article  Google Scholar 

  • Mahmoodzadeh H, Nabavi M, Kashefi H (2015) Effect of nanoscale titanium dioxide particles on the germination and growth of canola (Brassica napus). J Ornam Plants 3:25–32

    Google Scholar 

  • Martínez-Fernández D, Barroso D, Komárek M (2016) Root water transport of Helianthus annuus L. under iron oxide nanoparticle exposure. Environ Sci Pollut Res 23:1732–1741

  • Martínez-Fernández D, Vítková M, Michálková Z, Komárek M (2017) Engineered Nanomaterials for Phytoremediation of Metal/Metalloid-Contaminated Soils: Implications for Plant Physiology. In: Ansari A, Gill S, Gill RR, Lanza G, Newman L (eds) Phytoremediation. Springer, Cham. https://doi.org/10.1007/978-3-319-52381-1_14

  • Pariona N, Martínez AI, Hernandez-Flores H, Clark-Tapia R (2017) Effect of magnetite nanoparticles on the germination and early growth of Quercus macdougallii. Sci Total Environ 575:869–875

    Article  CAS  Google Scholar 

  • Plaksenkova I, Jermaļonoka M, Bankovska L, Gavarāne I, Gerbreders V, Sledevskis E, Sniķeris J, Kokina I (2019) Effects of Fe3O4 nanoparticle stress on the growth and development of rocket Eruca sativa. J Nanomater 2019:1–10

    Article  Google Scholar 

  • Purvis AC (1980) Sequence of chloroplast degreening in calamondin fruit as influenced by ethylene and AgNO3. Plant Physiol 66:624–627

    Article  CAS  Google Scholar 

  • Pyc M, Cai Y, Greer MS, Yurchenko O, Chapman KD, Dyer JM, Mullen RT (2017) Turning over a new leaf in lipid droplet biology. Trends Plant Sci 22:596–609

    Article  CAS  Google Scholar 

  • Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:208–212

    Article  CAS  Google Scholar 

  • Rico CM, Majumdar S, Duarte-Gardea M, Peralta-Videa JR, Gardea-Torresdey JL (2011) Interaction of nanoparticles with edible plants and their possible implications in the food chain. J Agric Food Chem 59:3485–3498

    Article  CAS  Google Scholar 

  • Sabo-Attwood T, Unrine JM, Stone JW, Murphy CJ, Ghoshroy S, Blom D, Bertsch PM, Newman LA (2012) Uptake, distribution and toxicity of gold nanoparticles in tobacco (Nicotiana xanthi) seedlings. Nanotoxicology 6:353–360

    Article  CAS  Google Scholar 

  • Stampoulis D, Sinha SK, White JC (2009) Assay-dependent phytotoxicity of nanoparticles to plants. Environ Sci Technol 43:9473–9479

    Article  CAS  Google Scholar 

  • Sz˝oll˝osi R, Molnár Á, Kondak S, Zsuzsanna Kolbert Z (2020) Dual effect of nanomaterials on germination and seedling growth: stimulation vs. phytotoxicity. Plants 9:1745

    Article  Google Scholar 

  • Trujillo-Reyes J, Majumdar S, Botez CE, Peralta-Videa JR, Gardea-Torresdey JL (2014) Exposure studies of core-shell Fe/Fe3O4 and Cu/CuO NPs to lettuce (Lactuca sativa) plants: are they a potential physiological and nutritional hazard? J Hazard Mater 267:255–263

    Article  CAS  Google Scholar 

  • Vargas-Hernandez M, Macias-Bobadilla I, Guevara-Gonzalez RG, Romero-Gomez SDJ, Rico-Garcia E, Ocampo-Velazquez RV, Alvarez-Arquieta LDL, Torres-Pacheco I (2017) Plant hormesis management with biostimulants of biotic origin in agriculture. Front Plant Sci 8:1762

    Article  Google Scholar 

  • Wang H, Kou X, Pei Z, Xiao JQ, Shan X, Xing B (2011) Physiological effects of magnetite (Fe3O4) nanoparticles on perennial ryegrass (Lolium perenne L.) and pumpkin (Cucurbita mixta) plants. Nanotoxicology 5:30–42

    Article  Google Scholar 

  • Yang Y, Benning C (2018) Functions of triacylglycerols during plant development and stress. Curr Opin Biotechnol 49:191–198

    Article  CAS  Google Scholar 

  • Youssef MS, Elamawi RM (2020) Evaluation of phytotoxicity, cytotoxicity, and genotoxicity of ZnO nanoparticles in Vicia faba. Environ Sci Pollut Res 27:18972–18984

    Article  CAS  Google Scholar 

  • Yuan J, Chen Y, Li H, Lu J, Zhao H, Liu M, Nechitaylo GS, Glushchenko NN (2018) New insights into the cellular responses to iron nanoparticles in Capsicum annuum. Sci Rep 19:3228

    Article  Google Scholar 

  • Zheng L, Hong F, Lu S, Liu C (2005) Effect of nano-TiO2 on strength of naturally aged seeds and growth of spinach. Biol Trace Elem Res 104:83–91

    Article  CAS  Google Scholar 

  • Zhu H, Han J, Xiao JQ, Jin Y (2008) Uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. J Environ Monit 10:713–717

    Article  CAS  Google Scholar 

  • Zuverza-Mena N, Martínez-Fernández D, Du W, Hernandez-Viezcas JA, Bonilla-Bird N, López-Moreno ML, Komárek M, Peralta-Videa JR, Gardea-Torresdey JL (2017) Exposure of engineered nanomaterials to plants: insights into the physiological and biochemical responses—a review. Plant Physiol Biochem 110:236–264

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank Ms. Duaa Qattan and Kholoud Friehat for the use of the TEM facility and for the technical assistance, Faculty of Medicine, The University of Jordan, Amman, Jordan.

Funding

This work was financially supported by the Deanship of Scientific Research at Jordan University of Science and Technology, for their generous grant No. 20160270. The funding body had no role in the design of the study and no role in the collection, analysis, and interpretation of data or in writing the manuscript.

Author information

Authors and Affiliations

  1. Department of Biotechnology and Genetic Engineering, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Rami Alkhatib

  2. Molecular Biology Program, New Mexico State University, Las Cruces, NM, 88003, USA

    Batool Alkhatib

  3. Department of Public Health, Faculty of Medicine, Jordan University of Science and Technology, Irbid, 22110, Jordan

    Nour Abdo

Authors
  1. Rami Alkhatib
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Batool Alkhatib
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nour Abdo
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

RA and BA designed the work, performed plant growth and sampling, and performed all parameters’ measurements used in this project. NA analyzed data and participates in the interpretation of data. All authors contributed to drafting and critically revising of the paper, gave final approval of the version to be published, and agreed to be accountable for all aspects of the work.

Corresponding author

Correspondence to Rami Alkhatib.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.

Additional information

Responsible Editor: Gangrong Shi

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Alkhatib, R., Alkhatib, B. & Abdo, N. Effect of Fe3O4 nanoparticles on seed germination in tobacco. Environ Sci Pollut Res 28, 53568–53577 (2021). https://doi.org/10.1007/s11356-021-14541-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-021-14541-x

Keywords

Search

Navigation