Abstract
The residues of dichlorodiphenyltrichloroethanes (DDTs) and hexachlorocyclohexanes (HCHs) in forty-eight (48) composite fruit and vegetable samples (carrot, cucumber, tomato, and watermelon) were qualitatively and quantitatively determined using gas chromatography equipped with electron capture detector (GC-ECD). Safety indices such as the estimated average daily intake (EADI), cancer benchmark concentration (CBC), hazard quotient (HQ) and index (HI), and hazard ratio (HR) were also estimated. Results showed the predominance of HCH and its isomers in the fruits and vegetables as compared with the DDTs. The most predominant HCHs in all the fruits was α-HCH with mean concentrations of 2.704 ± 2.488 mg kg−1 (carrot), 1.536 ± 1.036 mg kg−1 (cucumber), 6.814 ± 2.967 mg kg−1 (tomato), and 9.241 ± 2.735 mg kg−1 (watermelon). The analysis showed that the levels of pesticide residues detected in 25 to 100% of the fruit and vegetable samples were above the UK/European Commission Maximum Residue Limits (MRLs). The non-carcinogenic health risk estimates showed that only α-HCH in tomatoes and watermelon had HQ > 1 which indicates the possibility of systemic health risk in children consumers. The carcinogenic health risk showed that only α-HCH and γ-HCH in children and α-HCH in adults had HR > 1 for tomato and watermelon which implies the possibility of carcinogenic health risk from its consumption. It is therefore paramount to institute a policy and regulatory framework for regular monitoring of pesticide residues in fruits and vegetables in Nigeria in order to ensure food safety for consumers and protect human health.
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References
Aamir M, Khan S, Li G (2018) Dietary exposure to HCH and DDT congeners and their associated cancer risk based on Pakistani food consumption. Environ Sci Pollut Res 25(9):1–6. https://doi.org/10.1007/s11356-017-1129-1
Adeleye AO, Sosan MB, Oyekunle JAO (2019) Occurrence and human health risk assessment of DDT and HCH insecticide residues in commonly consumed vegetables in southwestern Nigeria. J Health Pollut 9(23):1–14. https://doi.org/10.1016/10.5696/2156-9614-9.23.190909
Adeyeye A, Osibanjo O (1999) Residues of organochlorine pesticides in fruits, vegetables and tubers from Nigerian markets. Sci Total Environ 231:227–233
Agency for Toxic Substances and Disease Registry (ATSDR) (2002) Toxicological profile for DDT/DDE/DDD, draft for public comment. U.S. Department of Health and Human Services. Available at https://www.atsdr.cdc.gov/substances/toxsubstance.asp?toxid=20. Accessed on 24 July 2020
Agency for Toxic Substances and Disease Registry (ATSDR) (2005) Toxicological profile for hexachlorocyclohexane. U.S. Department of Health and Human Services, Public Health Service, Atlanta https://www.atsdr.cdc.gov/substances/toxsubstance.asp?toxid=138. Accessed on 24 July 2020
Ahmed G, Anawar HM, Chibua DT, Takuwa IT, Sing GS, Sichilongo K (2015) Environmental assessment of fate, transport and persistent behavior of dichlorodiphenyltrichloroethanes and hexachlorocyclohexanes in land and water ecosystems. Int J Environ Sci Technol 12:2741–2756. https://doi.org/10.1007/s13762-015-0792-3
Akan JC, Mahmud MM, Waziri M, Mohammed Z (2015) Residues of organochlorine pesticides in watermelon (Citrulus lanatus) and soil samples from Gashua, Bade Local Government Area Yobe State, Nigeria. Adv Anal Chem 5(3):61–68. https://doi.org/10.5923/j.aac.20150503.03
Bankar R, Ray AK, Kumar A, Adeppa K, Puri S (2012) Organochlorine pesticide residues in vegetables of three major markets in Uttar Pradesh, India. Acta Biologica Indica 1(1):77–80
Bempah CK, Buah-Kwofie A, Enimil E, Blewub B, Agyei-Martey G (2012) Residues of organochlorine pesticides in vegetables marketed in Greater Accra Region of Ghana. Food Control 25:537–542. https://doi.org/10.1016/j.foodcont.2011.11.035
Bempah CK, Donkor A, Yeboah OP, Dubey B, Osei-fosu P (2011) A preliminary assessment of consumer’ s exposure to organochlorine pesticides in fruits and vegetables and the potential health risk in Accra Metropolis. Ghana Food Chem 128(4):1058–1065. https://doi.org/10.1016/j.foodchem.2011.04.013
Benson NU, Aruwajoye OI (2011) Assessment of contamination by organochlorine pesticides in Solanum lycopersicum L. and Capsicum annuum L. A market survey in Nigeria. Afr. J. Environ. Sci. Technol 5(6):437–442
Bhanti M, Taneja A (2005) Monitoring of organochlorine pesticide residues. Environ Monit Assess 110:341–342. https://doi.org/10.1007/s10661-005-8043-6
Boateng GO, Amuzu KK (2013) Levels of organochlorine pesticides residues in cabbage cultivated in farms along River Oyansia, Accra-Ghana. Am J Sci Ind Res 4(5):489–498. https://doi.org/10.5251/ajsir.2013.4.5.489.498
Bolor VK, Boadi NO, Borquaye LS, Afful S, (2018) Human risk assessment of organochlorine pesticide residues in vegetables from Kumasi, Ghana. J Chem 1–11. https://doi.org/10.1155/2018/3269065
Bundotich J, Gichuhi M (2015) Acute poisoning in the Rift Valley Provincial General Hospital, Nakuru, Kenya: January to June 2012. S Afr Fam Pract 57(3):214–218. https://doi.org/10.1080/20786190.2014.975448
Charlier C, Albert A, Herman P, Harmoir E, Gaspard E, Meurisse M, Plomteux G (2003) Breast cancer and serum organochlorine residues. Occup Environ Med 60(5):348–351
Cohn BA, Wolff MS, Cirillo PM, Sholtz RI (2007) DDT and breast cancer in young women: new data on the significance of age at exposure. Environ Health Perspect 115(10):1406–1414. https://doi.org/10.1289/ehp.10260
Dayasiri KC, Jayamanne SF, Jayasinghe CY (2017) Patterns of acute poisoning with pesticides in the paediatric age group. Int J Emerg Med 10(1):22. https://doi.org/10.1186/s12245-017-0148-5
Dougherty CP, Holtz SH, Reinert JC, Panyacosit L, Axelrad DA, Woodruff TJ (2000) Dietary exposures to food contaminants across the United States. Environ Res 84:170–185. https://doi.org/10.1006/enrs.2000.4027
European Commission (EC), (2017) Guidance document on analytical quality control and method validation procedures for pesticide residues and analysis in food and feed. SANTE/11813/2017. https://ec.europa.eu/food/sites/food/files/plant/docs/pesticidesmrlguidelineswrkdoc2017-11813.pdf. Accessed on 24 July 2020.
FAOSTAT (2020) Statistics of production crops for all area of the world (Nigeria). Available from: http://www.fao.org/faostat/en/#data/SC. Accessed on 20 December 2020.
Forkuoh F, Boadi NO, Borquaye LS, Afful S (2018) Risk of human dietary exposure to organochlorine pesticide residues in fruits from Ghana. Sci Rep 8(16686):1–5. https://doi.org/10.1038/s41598-018-35205-w
Friedman M (2013) Anticarcinogenic, cardioprotective, and other health benefits of tomato compounds: lycopene, α- tomatine, and tomatidine in pure form and in fresh and processed tomatoes. J Agric Food Chem 61:9534–9550. https://doi.org/10.1021/jf402654e
González-Andrade F, López-Pulles R, Estévez E (2010) Acute pesticide poisoning in Ecuador: a short epidemiological report. J Public Health 18(5):437–442. https://doi.org/10.1007/s10389-010-0333-ys
Gupta SK, Peshin SS, Srivastava A, Kaleekal T (2003) A study of childhood poisoning at national poisons information centre, All India Institute of Medical Sciences, New Delhi. J Occup Health 45(3):191–196. https://doi.org/10.1539/joh.45.191
Henny CJ, Grove RA, Kaiser JL (2008) Osprey distribution, abundance, reproductive success and contaminant burdens along lower Columbia river, 1997/1998 versus 2004. Arch Environ Contam Toxicol 54(3):525–534. https://doi.org/10.1007/s00244-007-9041-1
International Agency for Research on Cancer (IARC) (2018) Agents classified by the World Health Organization and International Agency for Research on Cancer Monograph Volumes 1-122, 17pp. Available at https://monographs.iarc.fr/wp-content/uploads/2018/09/List_of_Classifications.pdf. Accessed on 24 July 2020
Issa AB, Yassin K, Loutfy N, Ahmed MT (2018) Risk assessment of some organic contaminants: a case study based on food consumption in Tanta and Ismailia cities, Egypt. Environ Sci Pollut Res Int 25(34):34212–34220
Jardim ANO, Caldas ED (2012) Brazilian monitoring programmes for pesticide residues in food—results from 2001 to 2010. Food Control 25(2):607–616. https://doi.org/10.1016/j.foodcont.2011.11.001
Jayaraj R, Megha P, Sreedev P (2016) Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment. Interdiscip Toxicol 9:90–100. https://doi.org/10.1515/intox-2016-0012
Kolani L, Mawussi G, Sanda K (2016) Assessment of organochlorine pesticide residues in vegetable samples from some agricultural areas in Togo. Am. J Anal Chem 7:332–341. https://doi.org/10.4236/ajac.2016.74031
Kumar B, Verma VK, Naskar AK, Chakraborty P, Kumar S, Mukherjee D (2013) Human health risk from hexachlorocyclohexane and dichlorodiphenyl-trichloroethane pesticides through consumption of vegetables: estimation of daily intake and hazard quotients. J Xenobiot 3(6):29–35. https://doi.org/10.4081/xeno.2013.e6
Lee WJ, Ko Y, Cha ES (2013) Acute pesticide poisoning among children in South Korea: findings from National Health Insurance Claims Data, 2006-2009. J Trop Pediatr 60(1):4–9. https://doi.org/10.1093/tropej/fmt067
Lekei E, Ngowi AV, London L (2017) Acute pesticide poisoning in children: hospital review in selected hospitals of Tanzania. J Toxicol, 1–8. https://doi.org/10.1155/2017/4208405
Lippi G, Targher G (2011) Tomatoes, lycopene-containing foods and cancer risk. Br J Cancer 104:1234–1235. https://doi.org/10.1038/bjc
Mahugija JAM, Kayombo A, Peter R (2017) Pesticide residues in raw and processed maize grains and flour from selected areas in Dar es Salaam and Ruvuma, Tanzania. Chemosphere 185:137–144
Nakata H, Kawazoe M, Arizono K, Abe S, Kitano T, Shimada H, Li W, Ding X (2002) Organochlorine pesticides and polychlorinated biphenyl residues in foodstuffs and human tissues from China: status of contamination, historical trend, and human dietary exposure. Arch Environ Contam Toxicol 43:473–480. https://doi.org/10.1007/s00244-002-1254-8
National Population Commission (NPC) (2006) Nigeria’s National Census, Abuja, Nigeria. Available online at http://population.gov.ng/core-activities/surveys/dataset/2006-phc-priority-tables/.. Accessed on 20 December 2020
Olatunji OS (2019) Evaluation of selected polychlorinated biphenyls (PCBs) congeners and dichlorodiphenyltrichloroethane (DDT) in fresh root and leafy vegetables using GC-MS. Sci Rep 9(538):1–10. https://doi.org/10.1038/s41598-018-36996-8
Osei-Fosu P, Donkor AK, Ziwu C, Dubey B, Kingsford-Adaboh R, Asante IK, Nyarko S, Tawaih R, Nazzah NK (2017) Surveillance of pesticide residues in fruits and vegetables from Accra Metropolis markets, Ghana, 2010–2012: a case study in Sub-Saharan Africa. Environ Sci Pollut Res 24:17187–17205. https://doi.org/10.1007/s11356-017-9287-8
Owago JO, Shihua Q, Xing X, Zhang Y, Muhayimana AS (2006) Residues of organochlorine pesticides in vegetables from Deyang and Yanting Areas of the Chengdu Economic Region, Sichuan Province, China. Am J Sci 5(4):91–100
Oyekunle JAO, Ogunfowokan AO, Torto N, Akanni MS (2011) Determination of organochlorine pesticides in the agricultural soil of Oke-Osun farm settlement, Osogbo, Nigeria. Environ Monit Assess 177:51–61. https://doi.org/10.1007/s10661-010-1617-y
Oyeyiola AO, Fatunsin OT, Akanbi LM, Fadahunsi DE, Moshood MO (2017) Human health risk of organochlorine pesticides in foods grown in Nigeria. J Health Pollut 7(15):63–70
Ozcan C (2016) Determination of organochlorine pesticides in some vegetable samples using GC-MS. Pol J Environ Stud 25(3):1141–1147. https://doi.org/10.15244/pjoes/61627
Rahmawati S, Kirana LC, Yoneda M, Oginawati K (2017) Risk analysis on organochlorine pesticides residue in potato and carrot from conventional and organic farms in Citarum Watershed Area, West Java Province, Indonesia. J Environ Sci Technol 9(1):1–15
Regassa C, Tolcha T, Gomoro K, Megersa N (2020) Determination of residue levels of DDT and its metabolites in Khat and cabbage samples using QuEChERS sample preparation method combined with GC-MS detection. Ethiop J Sci Sustain Dev 7(1):1–10
SAS Institute (2003). SAS/STAT users guide. Release V9.2. SAS Institute, Cary.
Shoiful A, Fujita H, Watanabe I, Honda K (2013) Concentrations of organochlorine pesticides (OCPs) residues in foodstuffs collected from traditional markets in Indonesia. Chemosphere 90(5):1742–1750. https://doi.org/10.1016/j.chemosphere.2012.10.022
Slavin J, Lloyd B (2012) Health benefits of fruits and vegetables. Adv Nutr 3(4):506–516. https://doi.org/10.3945/an.112.002154
Sosan MB, Oyekunle JAO (2017) Organochlorine pesticide residue levels and potential human health risks in kolanut from selected markets in Osun state, Southwestern Nigeria. Asian J Chem Sci 2(4):1–11
Sosan MB, Oyekunle JAO, Odewale GO (2018) Occurrence and levels of organochlorine pesticide residues in maize samples from open markets and stores in Ile-Ife and Ondo, Southwestern Nigeria. Niger J Entomol 34:25–37. https://doi.org/10.36108/NJE/8102/43(0140)
Sosan MB, Oyekunle JAO, Olufade YA (2015) Dichloro-diphenyl-trichloro-ethane (DDT) and hexachlorohexane (HCH) pesticide residues in foodstuffs from markets in Ile-Ife, Nigeria. Int J Biol Chem Sci 9(1):442–453
Sosan MB, Adeleye AO, Oyekunle JAO, Udah O, Oloruntunbi PM, Daramola MO, Saka WT (2020) Dietary risk assessment of organochlorine pesticide residues in maize-based complementary breakfast food products in Nigeria. Heliyon 6(12):e05803. https://doi.org/10.1016/j.heliyon.2020.e05803
Stockholm Convention (2012) Success stories of Stockholm convention (2001-2012) Available online at. http://chm.pops.int/portals/0/download.aspx/UNEP-POPS-PAWA-SUCSTORY-2001-10LR.EN.PDF. Accessed on 20 December 2020
Stocka J, Biziuk M, Namiesnik J (2016) Analysis of pesticide residue in fruits and vegetables using analytical protocol based on application of the QuEChERS technique and GC-ECD system. Int J Glob Environ 15(1/2):136–150. https://doi.org/10.1504/IJGENVI.2016.074361
Tarazona-Díaz MP, Alacid F, Carrasco M, Martínez I, Aguayo E (2013) Watermelon juice: potential functional drink for sore muscle relief in athletes. J Agric Food Chem 61(31):7522–7528. https://doi.org/10.1021/jf400964r
Thomas JE, Ou LT, Al-Agely A (2008) DDE remediation and degradation. Rev Environ Contam Toxicol 194:55–69. https://doi.org/10.1007/978-0-387-74816-03
UK/EC (2008) Maximum residue levels in commodities set by United Kingdom and European commission. Available online at. https://secure.pesticides.gov.uk/MRLs/search.asp. Accessed on 20 July 2020
United Nations Environment Program, (UNEP), (2011) Draft revised guidance on the global monitoring plan for persistent organic pollutants, UNEP/POPS/COP.5/INF/27. Geneva, Switzerland. Available online at. http://chm.pops.int/Portals/0/download.aspx?d¼UNEP-POPS-COP.6-INF-25.English.pdf. Accessed on 24 July 2020
USEPA (2005) Guidance on selecting age groups for monitoring and assessing childhood exposures to environmental contaminants. Washington D.C. Environmental Protection Agency; 2005 50 p. https://www.epa.gov/sites/production/files/2013-09/documents/agegroups.pdf. Accessed on 20 July 2020
USEPA (2008) Child-specific exposure factors handbook (final report). Washington D.C.: Environmental Protection Agency; 687 p. Report No.: EPA/600/R-06/096F. Available from: https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=199243. Accessed on 24 July 2020
USEPA-IRIS (2019) Integrated risk information system (IRIS) assessments: list A to Z chemical assessments. Environmental Protection Agency, Washington. Available from: https://iris.epa.gov/AtoZ/?list_type=alpha. Accessed on 24 July 2020
Wang HS, Sthiannopkao S, Du J, Chen ZJ, Kim KW, Yasin MSM, Hashim JH, Wong CKC, Wong MH (2011) Daily intake and human risk assessment of organochlorine pesticides (OCPs) based on Cambodian market basket data. J Hazard Mater 192(3):1441–1449. https://doi.org/10.1016/j.jhazmat.2011.06.062
Wang N, Jiang Q, Han L, Zhang H, Zhu B, Liu X (2019) Epidemiological characteristics of pesticide poisoning in Jiangsu Province, China, from 2007 to 2016. Sci Rep 9(1):8604. https://doi.org/10.1038/s41598-019-44986-7
Wehner TC (2008) Watermelon. Handbook of plant breeding. Volume 1, Vegetables. I, Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae. Springer. pp. 381–418. https://doi.org/10.1007/978-0-387-30443-4_12.
WHO (2003) Inventory of IPCS and other WHO pesticide evaluations and summary of toxicological evaluations performed by the Joint Meeting on Pesticide Residues (JMPR) : evaluations through 2002, 7th ed. World Health Organization. https://apps.who.int/iris/handle/10665/67734. Accessed on 24 July 2020
WHO (2012a) Country assignments to the 17 proposed GEMS/ food consumption cluster diets of World Health Organization. Available at http://www.who.int/foodsafety/chem/cluster_diets_2012.pdf. Accessed on 24 July 2020
WHO (2012b) GEMS/Food consumption database for all 17 GEMS/cluster diets of World Health Organization 8pp. Available at http://www.who.int/nutrition/landscapeanalysis/nlisn_gem_food/en. Accessed on 24 July 2020
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The technical assistance by Mrs Olaoluwa Ruth Ojo of the Department of Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria, is immensely acknowledged.
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GOO designed the study, performed the experiment, performed the statistical analysis, and wrote the first draft of the manuscript. MBS conceptualised and supervised the study and appraised the data quality. JAOO reviewed the study and assisted in extraction protocols of pesticide residues from the samples. AOA managed literature searches and assisted in data analysis and interpretations. All authors read and approved the final manuscript
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Odewale, G.O., Sosan, M.B., Oyekunle, J.A.O. et al. Human health risk assessment of dichlorodiphenyltrichloroethane (DDT) and hexachlorocyclohexane (HCH) pesticide residues in fruits and vegetables in Nigeria. Environ Sci Pollut Res 28, 33133–33145 (2021). https://doi.org/10.1007/s11356-021-12747-7
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DOI: https://doi.org/10.1007/s11356-021-12747-7