Abstract
Glyphosate-based herbicides are widely used in global agriculture, and their effects on different non-target animal organisms have been the focus of many toxicological studies. Regarding the potential role of glyphosate-based herbicides as an endocrine disruptor, the present study aims to investigate the effects of the herbicide Roundup WG® (RWG) on female reproduction, specifically on the ovarian maturation of Danio rerio. Adult females were exposed to low concentrations of RWG (0.065, 0.65, and 6.5 mg L−1) for 15 days, and then the ovaries were submitted to structural and morphometric procedures, accompanied by analysis of the vitellin protein content. Our results showed an increase of initial ovarian follicle numbers, decrease of late ovarian follicles, and smaller diameter of ovarian follicles in fish exposed to 0.065 and 6.5 mg L−1. The thickness of vitelline envelope was reduced, and the vitellin protein content was increased in the ovarian follicle in the two highest concentrations. Ultrastructural changes in the ovarian follicular component were evident and expressed by the cell index; vacuolization in follicular cells, increase of perivitelline space, and impaired mitochondria in oocytes were observed. Therefore, RWG adversely affects the ovarian maturation in D. rerio, and these changes can lead to reproductive toxicity, compromising population dynamics.
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Acknowledgments
The authors are thankful to multi-user Laboratório de Estudos em Biologia/LAMEB and Laboratório Central de Microscopia/LCME of the Universidade Federal de Santa Catarina.
Funding
CE Davico was recipient of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/CAPES/Brazil scholarship. EM Nazari is recipient of productivity fellowship of Conselho Nacional de Desenvolvimento Científico e Tecnológico/Brazil (CNPq 311429/2017-3).
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CE Davico: designed the study, conducted the experiments, analyzed the data, and wrote the manuscript. AG Pereira: conducted the experiments, analyzed the data, and wrote the manuscript. L Nezzi: conducted the experiments. ML Jaramillo: conducted the experiments, analyzed the data, and wrote the manuscript. MS Melo: analyzed the data and wrote the manuscript. YMR Müller: designed the study, analyzed the data, and wrote the manuscript. EM Nazari: designed the study, analyzed the data, and wrote the manuscript.
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Highlights
- Lethal concentration50; 96 h for Roundup WG® for Danio rerio was 42.61 (± 3.6) mg L−1.
- Roundup WG® exposure induces alteration on number and diameter of ovarian follicles after 15 days.
- Critical ultrastructural alterations were induced by Roundup WG® in oocytes and vitelline envelope.
- Vitellin protein content increased in the ovarian follicle after Roundup WG® exposure for 15 days.
- Roundup WG® mainly affected the initial stages of ovarian maturation.
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Davico, C.E., Pereira, A.G., Nezzi, L. et al. Reproductive toxicity of Roundup WG® herbicide: impairments in ovarian follicles of model organism Danio rerio. Environ Sci Pollut Res 28, 15147–15159 (2021). https://doi.org/10.1007/s11356-020-11527-z
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DOI: https://doi.org/10.1007/s11356-020-11527-z