Skip to main content
SpringerLink
Log in

Comparative effects of zinc oxide and zinc oxide nanoparticle as feed additives on growth, feed choice test, tissue residues, and histopathological changes in broiler chickens

  • Research Article
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

Zinc is an essential nutritional trace mineral required for growth and health. The aim of the current work is to compare the effects of bulk zinc oxide and zinc oxide nanoparticles (ZnO-NPs) on the growth performance, feeding behavior, and zinc residues in tissues with the associated histopathological alterations in chicken. Meanwhile, the antibacterial activity against the isolated E. coli O78 strain was evaluated. Ninety Hubbard one-day-aged broiler chicks were divided into 3 groups, 30 each: birds of the 1st group fed a basal ration, those of the 2nd group fed a ration containing zinc oxide at a dose of 3000 mg/kg diet, and chicks of the 3rd group are given a ration containing ZnO-NPs at a dose of 90 mg/kg diet from 2 to 20 days age. The experiment lasted for 35 days. Feed choice test was done at the 3rd week of age on another 12 birds. Results revealed that birds fed with ZnO-NPs showed an improvement in body weight compared with the zinc oxide-administered group. There was no reluctance from birds to both diets supplemented with either zinc oxide or ZnO-NPs, with a constant preference to ZnO-NPs diet throughout the 5-min test. The highest levels of zinc were detected in the livers, kidneys, lungs, and muscles in the 1st day following cessation of drug administration. There was no significant effect on the levels of creatinine, uric acid, AST, and ALT. Mild to moderate degenerative changes as well as necrosis could be detected in the livers and hearts following both treatments. In conclusion, nano-zinc oxide could be practically used in broiler feed at a dose of 90 mg/kg diet instead of bulk zinc oxide, at a dose of 3000 mg/kg diet, with an improved body weight. Both supplements caused no significant effects on serum parameters and had the same antibacterial activity against E. coli O78.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmadi F, Ebrahimnezhad Y, Sis MN, Ghalehkandi GJ (2013) The effects of ZnO-NPs on performance, digestive organs and serum lipid concentrations in broiler chickens during starter period. Int J Biosci 3:23–29

    Article  Google Scholar 

  • Amara S, Slama IB, Mrad I, Rihane N, Khemissi W, El Mir L, Rhouma KB, Abdelmelek H, Sakly M (2014) Effects of zinc oxide nanoparticles and/or zinc chloride on biochemical parameters and mineral levels in rat liver and kidney. Hum Exp Toxicol 33(11):1150–1157

    Article  CAS  Google Scholar 

  • Asheer M, Manwar SJ, Gole MA, Sirsat S, Wade MR, Khose KK, Sajid Ali S (2018) Effect of dietary nano zinc oxide supplementation on performance and zinc bioavailability in broilers. Indian J Poult Sci 53(1):70–75; Research Article. https://doi.org/10.5958/0974-8180.2018.00004.1

    Article  Google Scholar 

  • Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A (2012) Antimicrobial activity of metal oxide nanoparticles against gram-positive and gram-negative bacteria: a comparative study. Int J Nanomedicine 7:6003–6009

    Article  CAS  Google Scholar 

  • Bancroft J, Gamble M (2008) Theory and practice of histological techniques, 6th edn. Churchill Livingstone Elsevier, Philadelphia

    Google Scholar 

  • Brooks MA, Grimes JL, Lloyd KE, Verissimo S, Speers JW (2013) Bioavailability in chicks of zinc from zinc propionate. J Appl Poultr Res 22:153–159

    Article  CAS  Google Scholar 

  • Chagneau AM, Bessonneau D, Bouchot C, Lescoat P, Picard M, Lessire M (2006) Broiler short-term feed preferences measured with SRAbox, a new feed choice procedure. Poult Sci 85:808–815

    Article  CAS  Google Scholar 

  • Chen J, Tan M, Nemmar A, Song W, Dong M, Zhang G, Li Y (2006) Quantification of extrapulmonary translocation of intratracheal-instilled particles in vivo in rats: effect of lipopolysaccharide. Toxicology 222:195–201

    Article  CAS  Google Scholar 

  • Collee CJ, Marmion BP, Fraser AG, Simmons A (1996) Cited in Mackie & Mccartney Practical medical microbiology, 4th edition page 151–178. New York EclinBurgh London

  • Davin R, Manzanilla EG, Figueroa J, SoláOriol D et al. (2011) Evaluation of the palatability of zinc oxide and zinc sulphate in the piglets diet. http://agris.fao.org/agrissearch/search.do?recordID=ES2011000280

  • European Commission (2003) Opinion of the Scientific Committee for Animal Nutrition on the use of zinc in feeding stuffs. HEALTH & CONSUMER PROTECTION DIRECTORATE-GENERAL Directorate C - Scientific Opinions C2 - Management of scientific committees; scientific co-operation and networks

  • Fossati P, Prencipe L, Berti G (1980) Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic systems in direct enzymatic assay of uric acid in serum and urine. Clin Chem 26:227–231

    Article  CAS  Google Scholar 

  • Fujihara J, Tongu M, Hashimoto H, Fujita Y, Nishimoto N, Yasuda T, Takeshita H (2015) Pro-inflammatory responses and oxidative stress induced by ZnO nanoparticles in vivo following intravenous injection. Eur Rev Med Pharmacol Sci 19:4920–4926

    CAS  Google Scholar 

  • FVE (Federation of Veterinarians of Europe) (2014) FVE position paper on the use of zincoxide.FVE/14/doc/016. https://www.knmvd.nl/.../016_fve_opinion_on_zinkoxide_adopted

  • Houot O (1985) In: Siest G, Henny J, Young DS (eds) Interpretation of clinical laboratory tests. Biochemical Publications, pp 220–234

  • Hu CH, Qian ZC, Song J, Luan ZS, Zuo AY (2013) Effects of zinc oxide-montmorillonite hybrid on growth performance, intestinal structure, and function of broiler chicken. Poultry 92:143–150

    Article  CAS  Google Scholar 

  • Hussein GAM, Khedr MH, Farghali AA (2002) Gadolinium oxide from gadolinium oxalate hydrate physicochemical characterization. Colloids Surf A Physicochem Eng Asp 203(1–3):137–142

    Article  CAS  Google Scholar 

  • Idowu OMO, Ajuwon RO, Oso AO, Akinloye OA (2011) Effects of zinc supplementation on laying performance, serum chemistry and Zn residue in tibia bone, liver, excreta and egg shell of laying hens. Int J Poult Sci 10:225–230

    Article  CAS  Google Scholar 

  • Iqbal A, Navarro M, Roura E (2018) Effectiveness of a double choice test to assess dietary taste preferences in broilers. https://zootecnicainternational.com/featured/effectiveness-double-choice-test-assess-dietary-taste-preferences-broiler-chickens-2/. Accessed 13 March 2018

  • Khan RU, Rahman ZU, Javed I, Muhammad F (2014) Effect of vitamins, protein level and probiotics on immune response of moulted male broilers. J Anim Physiol Anim Nutr 98(4):620–627. https://doi.org/10.1111/jpn.12087

    Article  CAS  Google Scholar 

  • Khedr MH, Farghali AA (2005) Physicochemical properties of nanocrystallite copper ferrite prepared by a novel self-flash combustion of acetate precursors. J Mater Sci Technol 21(5):675–680

    CAS  Google Scholar 

  • Kim WK, Patterson PH (2005) Effects of dietary zinc supplementation on hen performance, ammonia volatilization, and nitrogen retention in manure. J Environ Sci Health B 40:675–686

    Article  CAS  Google Scholar 

  • Li CH, Shen CC, Cheng YW, Huang SH, Wu CC, Kao CC, Liao JW, Kang JJ (2012) Organ biodistribution, clearance, and genotoxicity of orally administrated zinc oxide nanoparticles in mice. Nanotoxicology 6:746–756. https://doi.org/10.3109/17435390.2011.620717

    Article  CAS  Google Scholar 

  • Liu Y, He L, Mustapha A, Li H, Hu ZQ, Lin M (2009) Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157:H7. J Appl Microbiol 107:1193–1201

    Article  CAS  Google Scholar 

  • Luciane RS, Viégas J, Freitas LS, Brondani IL, Argenta FM et al (2011) Behavior patterns of cows with Charolais or Nellore breed predominance fed diets with plant extract or monensin sodium. Rev Bras Zootec l40(12):2954–2962

    Google Scholar 

  • Machebe NS, Iweh P, Onyimonyi AE, Ekere OS, Abonyi F (2013) Zinc oxide as an effective mineral for induced moulting: effects on post moult performance of laying hens in the humid tropics. J Vet Sci Technol S11:003. https://doi.org/10.4172/2157-7579.S11-003

    Article  Google Scholar 

  • Mahmoud UT, Abdel-Mohsein HS, Mahmoud MAM et al (2020) Effect of zinc oxide nanoparticles on broilers’ performance and health status. Trop Anim Health Prod. https://doi.org/10.1007/s11250-020-02229-2

  • Mavromichalis I, Peter CM, Parr TM, Ganessunker D, Baker DH (2000) Growth-promoting efficacy in young pigs of two sources of zinc oxide having either a high or a low bioavailability. J Anim Sci 78:2896–2902

    Article  CAS  Google Scholar 

  • NRC - National Research (1994) Council. Nutrient requirements of poultry.9thed.Washington: National Academic

  • Petrovic V, Nollet L, Kovac G (2010) Effect of dietary supplementation of trace elements on the growth performance and their distribution in the breast and thigh muscles depending on the age of broiler chickens. Acta Vet Brno 79:203–209

    Article  CAS  Google Scholar 

  • Picard M, Plouzeau M, Faure JM (1999) A behavioural approach to feeding broilers. Ann Zootech 48:233–245

    Article  Google Scholar 

  • Picard M, Le Fur C, Melcion J-P, Bouchot C (2000) Feed particles: what chickens see and touch. Productions Animales -Paris- Institut National de la RechercheAgronomique 13(2):117–130

    Article  Google Scholar 

  • Pousga S, Boly H, Ogle B (2005) Choice feeding of poultry: a review. Livest Res Rural Dev 17(4). http://www.lrrd.org/lrrd17/4/pous17045.htm. Accessed 1 April 2005

  • Qian JL (2011) The surface properties and photocatalytic activities of ZnO ultrafine particles. Appl Surf Sci 180:308–314

    Google Scholar 

  • Raghupathi KR, Koodali RT, Manna AC (2011) Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles. Langmuir. 5(27):4020–4028

    Article  Google Scholar 

  • Ramiah SK, Awad EA, Mookiah S, Idrus Z (2019) Effects of zinc oxide nanoparticles on growth performance and concentrations of malondialdehyde, zinc in tissues, and corticosterone in broiler chickens under heat stress conditions. Poult Sci 0:1–11. https://doi.org/10.3382/ps/pez093

    Article  CAS  Google Scholar 

  • Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63. https://doi.org/10.1093/ajcp/28.1.56

    Article  CAS  Google Scholar 

  • Reynolds FH, Forbes JM, Miller HM (2010) Does the newly weaned piglet select a zinc oxide supplemented feed, when given the choice? Animal 4(8):1359–1367. https://doi.org/10.1017/S1751731110000327

    Article  CAS  Google Scholar 

  • Salim HM, Lee HR, Jo C, Lee SK, Lee BD (2012) Effect of sex and dietary organic zinc on growth performance, carcass traits, tissue mineral content, and blood parameters of broiler chickens. Biol Trace Elem Res 147:120–129

    Article  CAS  Google Scholar 

  • Saman S, Moradhaseli S, Shokouhian A, Ghorbani M (2013) Histopathological effects of ZnO nanoparticles on liver and heart tissues in Wistar rats. Adv Biores 4:83–88

    CAS  Google Scholar 

  • Sarvari BGI, Seyedi AHII, Shahryar HAI, Sarikhan MI and Ghavidel SZI (2015) Effects of dietary zinc oxide and a blend of organic acids on broiler live performance, carcass traits, and serum parameters. Brazilian Journal of Poultry Science. Special Issue, Nutrition-Poultry Feeding Additives: 039–046 https://doi.org/10.1590/1516-635x

  • Seil JT, Webster TJ (2012) Antibacterial effect of zinc oxide nanoparticles combined with ultrasound. Nanotechnology 23(49):495101. https://doi.org/10.1088/0957-4484/23/49/495101

    Article  CAS  Google Scholar 

  • Su YK, Peng SM, Ji LW et al (2009) Ultraviolet ZnO nano rod photosensors. Langmuir 26:603–606

    Article  Google Scholar 

  • Tian L, Zhu FH, Ren HY, Jiang JY, Li W (2009) Effect of nano-zinc oxide on antioxidant function in broilers. Chin J Anim Nutr 21:534–539

    CAS  Google Scholar 

  • Varun A, Karthikeyan N, Muthusamy P, Raja A, Vijayarani K, Saranya S (2018) Effect of zinc oxide and zinc oxide nano particles on performance, carcass characteristics and metallotheionine (MTmRNA) expression in broiler chicken. Indian J Poult Sci 53(3):318–323

    Article  Google Scholar 

  • Watson CY, Molina RM, Louzada A, Murdaugh KM, Donaghey TC, Brain JD (2015) Effects of zinc oxide nanoparticles on Kupffer cell phagosomal motility, bacterial clearance, and liver function. Int J Nanomedicine 10:4173–4184. https://doi.org/10.2147/IJN.S82807

    Article  CAS  Google Scholar 

  • Xie Y, He Y, Irwin PL, Jin T, Shi X (2011) Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni. Appl Environ Microbiol 77:2325–2331

    Article  CAS  Google Scholar 

  • Zaboli K, Aliarabi H, Bahari AA, Abbasalipourkabir R (2013) 2013 Role of dietary nano-zinc oxide on growth performance and blood levels of mineral: A study on in Iranian Angora (Markhoz) goat kids. J Pharm Health Sci 2(1):19–26

    Google Scholar 

  • Zchelz Z, Moinar J, Hohmann J (2006) Antimicrobial and antiplasmid activities of essential oils. Fitoterapia 77:279–285

    Article  Google Scholar 

  • Zhao C, Tan S, Xiao X, Qiu X, Pan J, Tang Z (2014) Effects of dietary zinc oxide nanoparticles on growth performance and antioxidative status in broilers. Biol Trace Elem Res 160:361–367. https://doi.org/10.1007/s12011-014-0052-2

    Article  CAS  Google Scholar 

  • Zheng W, Jian YM, Zehang Y, Jiang W, Wang X, Cowan DM (2009) Chelation therapy of manganese intoxication with para amino salicylic acid in Sprague-Dawley rats. Toxicol Sci 54:295–301

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62515, Egypt

    Abeer M. Radi

  2. Department of Animal and Poultry Management and Wealth Development, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62515, Egypt

    Naglaa M. Abdel Azeem

  3. Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62515, Egypt

    EL-Shaymaa EL-Nahass

Authors
  1. Abeer M. Radi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Naglaa M. Abdel Azeem
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. EL-Shaymaa EL-Nahass
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to EL-Shaymaa EL-Nahass.

Additional information

Responsible editor: Mohamed M. Abdel-Daim

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Radi, A.M., Abdel Azeem, N.M. & EL-Nahass, ES. Comparative effects of zinc oxide and zinc oxide nanoparticle as feed additives on growth, feed choice test, tissue residues, and histopathological changes in broiler chickens. Environ Sci Pollut Res 28, 5158–5167 (2021). https://doi.org/10.1007/s11356-020-09888-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11356-020-09888-6

Keywords

Search

Navigation