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Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice

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Abstract

The laser pretreatment of seed is drawing pronounced attention from the scientific community for its positive impact in boosting germination, seedling, and growth of plants. In this study, the laser pretreatment of Adansonia digitata (A. digitata) seeds was evaluated. Eight laser treatments were conducted at different powers, 10, 20, 40, and 80 mW, with the two-time interval for each power at 2 and 4 min. The outcomes indicated that the most efficient irradiation was 10 mW/2 min which induces the highest germination rate and polyphenolic contents for seeds. Based on these results, the animal experimental design was processed to assess the hepatoprotective activity of A. digitata extracts obtained through the optimum laser preillumination to enhance the resistance of liver damage in mice. The total phenol and flavonoid contents and the antioxidant properties of the methanolic extracts were estimated in vitro. The CCl4 was used to induce hepatotoxicity in mice. The animals were divided into five groups. The sera of the treated animals were used for the determination of transaminases, and the liver homogenates were used for the determination of antioxidant status, and further liver tissues were subjected to verify the anti-apoptotic effect of A. digitata methanolic extract. The in vivo results showed that the methanolic extract exposed to laser treatment at 10 mW/2 min provided better hepatoprotective capacity than the other treatments. Administration of A. digitata extract not only offered a significant decrease in liver enzyme activity but also markedly improved the antioxidant status and reduced the apoptotic progression induced by CCl4 toxicity in liver tissue.

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Acknowledgments

We would like to thank Cairo University for the support and for providing the fund for this study.

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Authors and Affiliations

  1. Department of Laser Applications in Metrology, Photochemistry and Agriculture (LAMPA), National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt

    Galal Khamis & Manar Hassan

  2. Department of Medical Application of Lasers, National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt

    Mona Morsy

  3. Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

    Marwa A. Ibrahim & Mona Galal

  4. Department of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

    Reham M. Abd-Elsalam

  5. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt

    Shymaa Ahmed El Badawy & Asmaa A. Azouz

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  1. Galal Khamis
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  2. Manar Hassan
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  3. Mona Morsy
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  4. Marwa A. Ibrahim
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  5. Reham M. Abd-Elsalam
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  6. Shymaa Ahmed El Badawy
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  7. Asmaa A. Azouz
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  8. Mona Galal
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Correspondence to Marwa A. Ibrahim.

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Khamis, G., Hassan, M., Morsy, M. et al. Innovative application of helium-neon laser: enhancing the germination of Adansonia digitata and evaluating the hepatoprotective activities in mice. Environ Sci Pollut Res 27, 26520–26531 (2020). https://doi.org/10.1007/s11356-020-09036-0

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