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Myrtle (Myrtus communis) leaf extract suppresses hepatotoxicity induced by monosodium glutamate and acrylamide through obstructing apoptosis, DNA fragmentation, and cell cycle arrest

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Abstract

A large number of plant extracts have demonstrated to provide health benefits and mitigate several disease conditions. However, at the molecular and cellular levels, few studies have been conducted. The present work was designed to study the effect of Myrtus communis leaf extract (ME) (300 mg/kg bw) against hepatotoxicity induced by monosodium glutamate (MSG) (100 mg/kg bw), and acrylamide (ACR) (20 mg/kg bw) in male rats and determining its molecular and cellular mechanisms. The data showed that the treatment with MSG and/or ACR induced significant changes in numerous biomarkers (Bcl-2 and the programmed cell death protein-1) related to liver damage, as recorded by genotoxicity, apoptosis, and histopathological changes. On the other side, the oral administration of ME (300 mg/kg bw) improved the hepatic conditions as confirmed by the improvement in cell viability, programmed cell death, and histopathological alterations. It can be concluded that the consumption of ME might be useful for minimizing the occurred hepatotoxicity through up-regulation of the key apoptotic regulators as well as the improvement of DNA content and cell cycle restoration.

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Abbreviations

ME:

Myrtus communis leaf extract

MSG:

monosodium glutamate

ACR:

acrylamide

ROS:

redox oxygen species

PD-1:

programmed cell death protein-1

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Authors and Affiliations

  1. Department of Biology, Faculty of Science and Art, Taibah University, Al-Ula, Saudi Arabia

    Hanaa A. Hassan

  2. Physiology Division, Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt

    Hanaa A. Hassan, Wafaa M. EL-Kholy, Mamdouh R. F. EL-Sawi & Nadine A. Galal

  3. Agricultural Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt

    Mohamed Fawzy Ramadan

  4. Deanship of Scientific Research, Umm Al-Qura University, P.O. Box 715, Makkah, Saudi Arabia

    Mohamed Fawzy Ramadan

Authors
  1. Hanaa A. Hassan
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  2. Wafaa M. EL-Kholy
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  3. Mamdouh R. F. EL-Sawi
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  4. Nadine A. Galal
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  5. Mohamed Fawzy Ramadan
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Correspondence to Hanaa A. Hassan or Mohamed Fawzy Ramadan.

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Hassan, H.A., EL-Kholy, W.M., EL-Sawi, M.R.F. et al. Myrtle (Myrtus communis) leaf extract suppresses hepatotoxicity induced by monosodium glutamate and acrylamide through obstructing apoptosis, DNA fragmentation, and cell cycle arrest. Environ Sci Pollut Res 27, 23188–23198 (2020). https://doi.org/10.1007/s11356-020-08780-7

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