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Ginkgo biloba mitigates silver nanoparticles-induced hepatotoxicity in Wistar rats via improvement of mitochondrial biogenesis and antioxidant status

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A Correction to this article was published on 07 September 2019

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Abstract

Silver nanoparticles (AgNPs) are noble metal nanoparticles, due to their good physicochemical properties, which have been exploited in biological applications. Nanotechnological applications advance very quickly while few literatures assessed the effects of natural products on the risks of nanoparticles in vivo. Thirty male adult rats were enrolled equally into: control, AgNPs (50 mg/kg b.w i.p 3 times/week) and GBE (100 mg/kg b.w daily per os)+AgNPs. After 30 days, the assessment of liver function, antioxidative status, mitochondrial biogenesis, and histopathological analyses were performed. AgNP exposure enhanced the hepatic lipid peroxidation (+ 281.7%) along with a decline in the reduced glutathione (− 58.3%) levels. The apparent hepatic oxidative damage was associated with obvious hepatic dysfunction that was ascertained by alteration of serum liver enzymatic biomarkers, lipid profile, and pathological hepatic lesions. Following AgNP exposure, hepatic silver and calcium contents were increased without changes in the trace element concentrations. Finally, the mRNA transcripts of hepatic PGC-1α, mtTFA, and Nrf2 were downregulated after AgNP exposure. Interestingly, GBE has the ability to alleviate AgNP-induced hepatic damage assessed by augmentation of reduced glutathione level and mitochondrial biogenesis. This study explored the potential protective role of GBE on AgNPs-induced hepatotoxicity via attenuation of oxidative stress, substantial enhancement of cell viability with concomitant mitigating DNA damage, and mitochondrial dysfunction.

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  • 07 September 2019

    The original publication of this paper contains a mistake. The correct image of figure 3 is shown in this paper.

Abbreviations

AgNPs:

silver nanoparticles

Akt:

protein kinase B pathway

ALT:

alanine aminotransferase

ALP:

alkaline phosphatase

ANOVA:

analysis of variance

ARE:

antioxidant response element

AST:

aspartate aminotransferase

ATP:

adenosine triphosphate

CCl4:

carbon tetrachloride

ER:

endoplasmic reticulum

GBE:

Ginkgo biloba L. extract

GSH:

reduced glutathione

GSSG:

oxidized glutathione

HCl:

hydrochloric acid

HDL-c:

high-density lipoprotein cholesterol

HE:

hematoxylin and eosin

HNO3:

nitric acid

ICP-MS:

inductively coupled plasma mass spectrometry

LDL-c:

low-density lipoprotein cholesterol

MDA:

malondialdehyde

mtTFA:

mitochondrial transcription factor A

Nrf2:

nuclear factor erythroid 2 like factor

PGC-1α:

peroxisome proliferative activated receptor gamma coactivators 1 alpha

ROS:

reactive oxygen species

SE:

standard error

TAG:

Triacylglycerol

TEM :

transmission electron microscope

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt

    Eman M. Abd El-Maksoud, Mohamed A. Lebda, Aml E. Hashem & Nabil M. Taha

  2. Department of Biochemistry, Institute of Medical Research, Alexandria University, Alexandria, Egypt

    Maher A. Kamel

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  1. Eman M. Abd El-Maksoud
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  2. Mohamed A. Lebda
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  3. Aml E. Hashem
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  4. Nabil M. Taha
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  5. Maher A. Kamel
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Correspondence to Mohamed A. Lebda.

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The handling of rats were performed according to international ethical guidelines for the care and use of laboratory animals and the approval of experimental procedures were done by the Experimental Animal Use and Ethics Committee at the Faculty of Veterinary Medicine, Alexandria University, Egypt.

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Responsible editor: Philippe Garrigues

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Abd El-Maksoud, E.M., Lebda, M.A., Hashem, A.E. et al. Ginkgo biloba mitigates silver nanoparticles-induced hepatotoxicity in Wistar rats via improvement of mitochondrial biogenesis and antioxidant status. Environ Sci Pollut Res 26, 25844–25854 (2019). https://doi.org/10.1007/s11356-019-05835-2

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