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Histological and immunohistochemical evaluation of the effect of tartrazine on the cerebellum, submandibular glands, and kidneys of adult male albino rats

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Abstract

Tartrazine is one of the most widely used food additives. The present investigation was carried out on 40 adult male albino rats. They were divided into four groups of ten animals for each. Group I was considered as a control group. Group II was treated with tartrazine daily in a dose 7.5 mg/kg body weight by oral gavage for 30 days. Group III was received 15 mg/kg body weight of tartrazine for the same period. Group IV was administered tartrazine in a dose 100 mg/kg body weight for the whole duration of the experiment. At the end of experiment, samples from the cerebellum, submandibular salivary glands, and kidneys were fixed in neutral buffered formalin 10% and prepared routinely for paraffin sectioning and staining for histopathological and immunohistochemical investigations of proliferating cell nuclear antigen “PCNA” and glial fibrillar acidic protein “GFAP”. Tartrazine-treated groups revealed histopathological degenerative changes in the obtained organs. In group II, the cerebellum showed subcortical edema, congestion of the blood vessels, cytoplasmic vacuolations, and pyknosis of the nuclei in the gray matter neurons. Concerning the submandibular glands, they expressed cytoplasmic vacuolations and pyknosis of the nuclei of the acinar cells, congestion of the interacinar blood capillaries, and degenerative changes in the striated duct. The kidneys appeared with interstitial hemorrhage and dilatation of the glomerular capillaries. The PCT and DCT showed ill-defined cell boundaries. The collecting tubules in the renal medulla appeared with flattened epithelial cells. The severity of these changes increases by increasing the dose of tartrazine in group III and reach to the highest level in group IV. The immunoexpression of the GFAP in the cerebellum of the experimental groups was intense compared to the control group. The immunoreactivity of PCNA in the nuclei of the acinar and ductal cells of the submandibular gland and the cells of the renal cortex and medulla was strong in the tartrazine-treated groups compared to the control group. The current study concluded that the tartrazine had serious effect on the cerebellum, submandibular glands, and kidneys that adversely affect the functions of these organs.

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References

  • Abdin F (1981) Cell and tissue damage. In: Abdin’s General Pathology, 4th edn. p 7

  • Alaa Ali F, Sherein Abdelgayed SA, Osama El Tawil S, Adel Bakeer M (2016) Toxicological and histopathological studies on the effect of tartrazine in male albino rats. Int J Pharmacol Sci 10(8):527–532

    Google Scholar 

  • Amin KA, Hameid HA, Abd Elsttar AH (2010) Effect of food azo dyes tartrazine and carmoisine on biochemical parameters related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food Chem Toxicol 48:2994–2999

    Article  CAS  Google Scholar 

  • Babu S, Shenolikar S (1995) Health and nutritional implications of food colors. Indian J Med Res 102:245–249

    CAS  Google Scholar 

  • Bancroft JD, Gamble M (2008) Theory and practice of histological techniques, 6th edn. Churchill Livingston Edinburgh, London

    Google Scholar 

  • Cameron GR (1952) Pathology of the cell. Oliver and Boyd, Edinburgh

    Google Scholar 

  • Cardoso WP, Denardin OVP, Rapoport A, Araujo CV, Carvalho MB (2000) Proliferating cell nuclear antigen expression in mucoepidermoid carcinoma of salivary glands. Sao Paulo Med J 118:69–74

    Article  CAS  Google Scholar 

  • Cheon Y, Cho KJ, Song J, Kim GW (2016) Knockdown of apoptosis signal-regulating kinase 1 affects ischaemia-induced astrocyte activation and glial scar formation. Eur J Neurosci 43:912–922

    Article  Google Scholar 

  • Chung K (2000) Mutagenicity and carcinogenicity of aromatic amines metabolically produced from azo dyes. Environ Carcinog Ecotoxicol Rev 18:51–74

    Article  Google Scholar 

  • Chung KT, Stevens JRE, Cerniglia CE (1992) The reduction of dyes by the intestinal microflora. Crit Rev Microbiol 18(3):175–190

    Article  CAS  Google Scholar 

  • EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) (2016) Safety and efficacy of tartrazine (E 102) for cats and dogs, ornamental fish, grain-eating ornamental birds and small rodents. EFSA J 14(11):4613

    Google Scholar 

  • Elbanna K, Sarhan OS, Khider M, Elmogy M, Abulreesh HH, Shaaban MR (2017) Microbiological, histological, and biochemical evidence for the adverse effects of food azo dyes on rats. J Food Drug Anal 25:667–680

    Article  CAS  Google Scholar 

  • Elhakim M, Heraud F (2007) New considerations regarding the risk assessment on tartrazine: an updated toxicological assessment, intolerance reactions and maximum theoretical daily intake in France. Regul Toxicol Pharmacol 47:308–316

    Article  CAS  Google Scholar 

  • Eng LF, Smith ME (1985) Recent studies of the glial fibrillary acidic protein. Ann N Y Acad Sci 455:525–537

    Article  CAS  Google Scholar 

  • Eng LF, Yandrhaegan JJ, Bignami A, Gerstle B (1971) An acidic protein isolated from fibrous astrocytes. Brain Res 28:351–354

    Article  CAS  Google Scholar 

  • Franco R, Cidlowski JA (2009) Apoptosis and glutathione: beyond an antioxidant. Cell Death Differ 16:1303–1314

    Article  CAS  Google Scholar 

  • Gautam D, Gunjan S, Goyal RP (2010) Evaluation of toxic impact of tartrazine on male swiss albino mice. Pharmacol Online 1:133–140

    Google Scholar 

  • Ghonimi WAM, Elbaz A (2015) Histological changes of selected westar rat tissues following the ingestion of tartrazine with special emphasis on the protective effect of royal jelly and cod liver oil. Cytol Histol 6:4

    Google Scholar 

  • Henics T, Whealthy DN (1999) Cytoplasmic vacuolation, adaptation and cell death: a view on new perspectives and features. Biol Cell 91:485–498

    Article  CAS  Google Scholar 

  • Himri I, Bellaheen S, Souna F, Belmekki F, Aziz M, Bnouham M, Berkia Z, Mekhfi H, Saaluri E (2011) A 90-day oral toxicity study of tartrazine a synthetic food dye in wistar rats. Int J Pharm Pharmaceut Sci 3:159–169

    CAS  Google Scholar 

  • Hirschbruch MD, Torres E (1998) Toxicologia de alimentos: uma discussão, Hig Alim 12(53):21–25

  • Hook JB (1975) Toxic response of the kidney. In: Casarett and Doull’s toxicology. The basic science of poisons, 2nd edn. Macmillan Publishing Co., New York, p 237

    Google Scholar 

  • Hurtado RM (1998) Reacciones adversas a alimentos y sus aditivos. Pediatr Diabetes 14(3):128–131

    Google Scholar 

  • Imane H, Faiza S, Mohammed A, Abd Elkader H, Ennouamane S (2012) DNA damage induced by tartrazine in rat whole blood using comet assay (single cell gelelectrophoresis). Adv Environ Biol 6(11):2875–2881

    Google Scholar 

  • Itall PA, Levision DA, Woods AL, Yucc-w Kellock DB, Watkins JA (1990) Proliferating cell nuclear antigen (PCNA) immune localization in paraffin sections and index of cell proliferation with evidence of deregulated expression in some neoplasm. J Pathol 162:285–294

    Article  Google Scholar 

  • JECFA (Joint FAO/WHO expert committee on food additives (1964) Specifications for identity purity and toxicological evaluation of food colours in FAO nutrition. Meetings report series no 38B, WHO, Geneva

  • Jones DB (1985) Kidneys. In: Kissane JM (ed) Anderson’s pathology, 8th edn. St. Louis, The C.V. Mosby Company, p 730

    Google Scholar 

  • Jones R, Ryan AJ, Se W (1964) The metabolism and excretion of tartrazine. Food Cosmet Toxicol 2:447–452

    Article  CAS  Google Scholar 

  • Kaplan SH (1987) Pathology, 1st edn. sh Kaplan educational center LTD, pp 3–8

  • Lechner J, Krall M, Netzer A, Radmayr C, Ryan MP, Pfaller W (1999) Effects of interferon alpha-2b on barrier function and junctional complexes of renal proximal tubular LLC-PK1 cells. Kidney Int Jun 55(6):2178–2191

    Article  CAS  Google Scholar 

  • Liu R, Chiu HM, Shiau CS, Yeh RL, Hung YT (2007) Degradation and sludge production of textile dyes by Fenton and photo processes. Dyes Pig 73:1–6

    Article  CAS  Google Scholar 

  • Maekawa A, Matsuoka C, Onodera H, Tanigawa H, Furuta K, Kanno J, Jang JJ (1987) Lack of carcinogenecity of tartrazine (FD & C yellow n° 5) in the F344 rat. Food Chem Toxicol 25(12):891–896

    Article  CAS  Google Scholar 

  • Mehedi N, Ainad-Tabet S, Mokrane N, Addou S, Zaoui C, Kheroua O, Saidi D (2009) Reproductive toxicology of tartrazine (FD and C yellow no 5) in Swiss albino mice. Am J Pharm Toxicol 4(4):128–133

    Google Scholar 

  • Mehedi N, Mokrane N, Alami O, Ainad-Tabet S, Zaoui C (2013) A thirteen week ad libitum administration toxicity study of tartrazine in Swiss mice. Afr J Biotechnol 12:4519–4529

    Article  CAS  Google Scholar 

  • Mimnaugh EG, Xu W, Vos M, Yuan X, Neckers L (2006) Endoplasmic reticulum vacuolization and valosin- containing protein delocalization result from simultaneous hsp90 inhibition by geldanamycin and proteasome inhibition by velcade. Mol Cancer Res 4:667–681

    Article  CAS  Google Scholar 

  • Mohamed NA, Saleh SM (2010) Effect of pre and postnatal exposure to lead acetate on the kidney of male albino rat: a light and electron microscopic study. Egypt J Hist 33(2):365–379

    Google Scholar 

  • Mohamed AA, Galal AA, Elewa YH (2015) Comparative protective effects of royal jelly and cod liver oil against neurotoxic impact of tartrazine on male rat pups brain. Acta Histochem 117:649–658

    Article  CAS  Google Scholar 

  • Mollendorf A (1973) Cytology cell physiology, 3rd edn. Academic Press New, York

    Google Scholar 

  • Moubarak R (2008) The effect of hypercholesterolemia on the rat parotid salivary gland (histopathological and immunohistochemical study). Cairo Dental Journal 24(1):19–28

    Google Scholar 

  • Moutinho D, Bertges LC, Assis RVC (2007) Prolonged use of the food dye tartrazine (FD&C Yellow n° 5) and its effects on the gastric mucosa of wistar rats. Braz J Biol 67:141–145

    Article  CAS  Google Scholar 

  • Nakane PK, Moriuchi T, Koji T, Taniguchi Y, Izumi S, Hui L (1989) Proliferating cell nuclear antigen (PCNA/cyclin): review and some new findings. ACTA Histochem Cytochem J 22(1):105–116

    Article  Google Scholar 

  • Pustazi L, Lewis C, Lorenzen J, Megee J (1993) Growth factors: regulation of normal and neoplastic growth. J Pathol 169:191–201

    Article  Google Scholar 

  • Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405–426

    Article  CAS  Google Scholar 

  • Reyes FG, Valim MF, Vercesi AE (1996) Effect of organic synthetic food colours on mitochondrial respiration. Food Addit Contam 13(1):5–11

    Article  CAS  Google Scholar 

  • Roxon J, Ryan A, Wright S (1967) Enzymatic reduction of tartrazine by Proteus vulgaris from rats. Food Cosmet Toxicol 5:645–656

    Article  CAS  Google Scholar 

  • Rus V, Gherman C, Miclăuş V, Mihalca A, Nadăş GC (2009) Comparative toxicity of food dyes on liver and kidney in Guinea pigs: a histopathological study. Ann RSCB 15(1):161–165

    Google Scholar 

  • Sandler RS (1983) Diet and cancer: food additives, coffee and alcohol. Nutr Cancer 4(4):273–279

    Article  CAS  Google Scholar 

  • Sasaki YUF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S (2002) The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat Res 519:103–119

    Article  CAS  Google Scholar 

  • Sharma S, Goyal RP, Chakravarty G, Sharma A (2008) Toxicity of tomato red, a popular food dye blend on male albino mice. Exp Toxicol Pathol 60:51–57

    Article  CAS  Google Scholar 

  • Sternberger L (2006) Immunocytochemistiy, 3rd edn. John Wiley Medical, New York, pp 190–209

    Google Scholar 

  • Stevens A, Lowe J (1995) Pathology, 1st edn. Alan Stevens/James Lowe Mosby, Baltimore, pp 23–33

    Google Scholar 

  • Stoltenburg GD, Peters IPB, Herbst MMH, Weigand GWH (1996) Glial fibrillary acidic protein and RNA expression in adult rat hippocampus following low level lead exposure during development. Histochem Cell Biol 105:431–422

    Article  Google Scholar 

  • Tanaka T (2006) Reproductive and neuro behavioural toxicity study of tartrazine administered to mice in the diet. Food and Chem. Toxicology 44(2):179–187

    CAS  Google Scholar 

  • Tanaka T, Takahashi S, Oishi A, Ogata A (2008) Effects of tartrazine on exploratory behavior in a three-generation toxicity study in mice. Reprod Toxicol 26(2):156–163

    Article  CAS  Google Scholar 

  • Toledo MCF (1996) Aditivos alimentares in fundamentos de toxicología. Atheneu, Brasil, pp 405–39

  • Toledo MCF (1999) Regulamentação de uso de corantes naturais. Arch Latinoam Nutr 49(l1):67–70

    Google Scholar 

  • Tsai CF, Kuo CH, Shih DYC (2015) Determination of 20 synthetic dyes in chili powders and syrup-preserved fruits by liquid chromatography/tandem mass spectrometry. J Food Drug Anal 23:453–462

    Article  CAS  Google Scholar 

  • Tsuji T, Mimura Y, Wen S, Li X, Kanekawa A, Sasaki K, Shinozaki F (1995) The significance of PCNA and p53 protein in some oral tumors. Int J Oral Maxillofac Surg 24:221–225

    Article  CAS  Google Scholar 

  • Uesugi N, Furumiya K, Mizutani T (2006) Inhibition mechanism of UDP-glucuronosyl transferase 1A6 by xanthenes food dyes. J Health Sci 52:549–557

    Article  CAS  Google Scholar 

  • Volpi EL (1985) Aditivos alimentares. Aliment Nutr 6(23):40–44

    Google Scholar 

  • Walton K, Walker R, Jjm VDS, Castell JV (1999) The application of in vitro in the derivation of the acceptable daily intake of food additives. Food Chem Toxicol 37:1175–1197

    Article  CAS  Google Scholar 

  • Woolf N (2000) Cell, tissue and disease. The basis of pathology, 3rd edn. W.B. Saunders Company LTD, Edinburgh, pp 29–38

    Google Scholar 

  • Zhang G, Ma Y (2013) Mechanistic and conformational studies on the interaction of food dye amaranth with human serum albumin by multispectroscopic methods. Food Chem 136:442–449

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Cytology and Histology, Faculty of Vet. Med, Cairo University, Cairo, Egypt

    Mohamed A. El-sakhawy, Dina W Mohamed & Yasmine H. Ahmed

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  1. Mohamed A. El-sakhawy
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  2. Dina W Mohamed
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  3. Yasmine H. Ahmed
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Correspondence to Yasmine H. Ahmed.

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El-sakhawy, M.A., Mohamed, D.W. & Ahmed, Y.H. Histological and immunohistochemical evaluation of the effect of tartrazine on the cerebellum, submandibular glands, and kidneys of adult male albino rats. Environ Sci Pollut Res 26, 9574–9584 (2019). https://doi.org/10.1007/s11356-019-04399-5

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