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The effect of dietary selenium addition on the concentrations of heavy metals in the tissues of fallow deer (Dama dama L.) in Croatia

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Abstract

The aim of this research was to determine the concentrations of cadmium, lead, mercury, and arsenic and the essential elements iron and selenium in the tissues (muscle, kidney, liver, spleen, and fat) of fallow deer (Dama dama L.) without and with supplemental selenium addition. Another aim was to determine the effect of selenium addition on the indicators of oxidative stress, namely, the levels of superoxide dismutase, glutathione peroxidase, glutathione, and vitamin E. The research was carried out with 40 fallow deer during two research periods. Supplemental feed without selenium addition was provided during the first research period, and supplemental feed with added selenium (3 mg/kg) was provided for 60 days during the second research period. The concentration of selenium in tissues was higher in the second research period than in the first research period (in kidney tissue, 0.957 vs. 0.688 mg/kg, P < 0.05). The dietary addition of selenium decreased (P < 0.05) the concentrations of some heavy metals (lead in the spleen = 0.06 vs. 0.27 mg/kg and in the fatty tissue = 0.17 vs. 0.69 mg/kg; arsenic in the muscle tissue = 0.005 vs. 0.014 mg/kg, liver = 0.003 vs. 0.009 mg/kg, spleen = 0.004 vs. 0.013 mg/kg, and fat = 0.008 vs. 0.016 mg/kg). The activity of glutathione peroxidase was significantly higher (P < 0.05) in the second research period than in the first research period (1375.36 vs. 933.23 U/L).

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The authors express gratitude to the Croatian Hunting Association for providing support in funding this research.

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Authors and Affiliations

  1. Croatian Hunting Association, Vladimira Nazora 63, 10 000, Zagreb, Croatia

    Neška Vukšić & Ivica Budor

  2. J.J. Strossmayer University from Osijek Faculty of Agriculture in Osijek, Vladimira Preloga 1, 31 000, Osijek, Croatia

    Marcela Šperanda, Zdenko Lončarić & Mislav Đidara

  3. Veterinary Research Institute, Hudcova 296/70, 621 00, Brno, Czech Republic

    Eyer Ludek

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  1. Neška Vukšić
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  2. Marcela Šperanda
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Correspondence to Marcela Šperanda.

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Vukšić, N., Šperanda, M., Lončarić, Z. et al. The effect of dietary selenium addition on the concentrations of heavy metals in the tissues of fallow deer (Dama dama L.) in Croatia. Environ Sci Pollut Res 25, 11023–11033 (2018). https://doi.org/10.1007/s11356-018-1406-7

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