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Testing a ratio of photosynthesis to O3 uptake as an index for assessing O3-induced foliar visible injury in poplar trees

  • Ozone and plant life: the Italian state-of-the-art
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Abstract

Visible foliar injury by ozone (ozone visible injury) is known as a biomarker to assess potential phytotoxicity of ozone. We investigated ozone visible injury in an ozone-sensitive poplar (Oxford clone) under a 2-year free-air controlled exposure (FACE) experiment and calculated three ozone indices (i.e., accumulative ozone exposure over 40 ppb during daylight hours (AOT40), phytotoxic ozone dose above a flux threshold of 0 nmol m−2 s−1 (POD0), and the cumulative value of the ratio of hourly ozone uptake to net photosynthesis (ΣU/P n ) to assess the critical level (CL) at the time of the first symptom onset of ozone visible injury. We tested the hypothesis that ozone injury depends both on the amount of ozone entering a leaf and on the capacity for biochemical detoxification or repair with photosynthesis as a proxy. The CLs at the time of the first symptom onset of ozone visible injury were 19 ppm h for AOT40, 26 mmol m−2 for POD0, and 1.2 mol mol−1 for ΣU/P n in Oxford clone at the ozone FACE experiment. Our findings were then verified by 4-year observation-based data in central Italy on Oxford clone and white poplar (Populus alba L.). These observation-based data indicated that we found ozone visible injury in Oxford clone even though AOT40 was relatively low (11.7 ppm h). On the other hand, when values of POD0 and ΣU/P n exceeded over the CLs, the occurrence of initial symptoms in Oxford clone was shown. White poplar did not show ozone visible injury. ΣU/P n of white poplar at the field sites reached ~1.0 mol mol−1 (less than the CL = 1.2 mol mol−1, which was obtained from O3 FACE) during May–September, although the values of POD0 were relatively high in white poplar (44–47 mmol m−2 during May–September). The result implies that ozone injury may have occurred in poplars when stomatal ozone flux exceeded the critical range of tolerance due to the assimilate shortage for repair and defense against ozone stress.

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Acknowledgements

We would like to thank Moreno Lazzara for support during the field work, the Fondazione Cassa di Risparmio di Firenze (2013/7956) and the LIFE15 ENV/IT/000183 project MOTTLES for financial support to the experiment, and the National Natural Science Foundation of China (31401895) and China Scholarship Council (201606615002) for financial support to Lu Zhang for his visiting research in Italy.

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Authors and Affiliations

  1. Institute of Sustainable Plant Protection, National Research Council of Italy, Via Madonna del Piano 10, I-50019, Sesto Fiorentino, Italy

    Yasutomo Hoshika, Elisa Carrari, Sara Pignatelli & Elena Paoletti

  2. College of Horticulture, Northeast Agricultural University, 59 Mucai Street, Harbin, 150030, China

    Lu Zhang

  3. Institute of Biometeorology, National Research Council of Italy, Via Giovanni Caproni, 8, 50145, Florence, Italy

    Giulia Carriero

  4. Institute of Biometeorology, National Research Council of Italy, Via Madonna del Piano 10, I-50019, Sesto Fiorentino, Italy

    Gianni Fasano & Alessandro Materassi

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  1. Yasutomo Hoshika
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  2. Elisa Carrari
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  3. Lu Zhang
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Correspondence to Yasutomo Hoshika.

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Hoshika, Y., Carrari, E., Zhang, L. et al. Testing a ratio of photosynthesis to O3 uptake as an index for assessing O3-induced foliar visible injury in poplar trees. Environ Sci Pollut Res 25, 8113–8124 (2018). https://doi.org/10.1007/s11356-017-9475-6

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