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Structure and function of the bacterial communities during rhizoremediation of hexachlorobenzene in constructed wetlands

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Abstract

Vertical flow constructed wetlands (VF CWs) are considered to be effective for treating organic pollutants. The rhizosphere of macrophytes such as Phragmites sp., Typha sp. serves as an active and dynamic zone for the microbial degradation of organic pollutants. However, it is still not clear how soil bacterial communities respond to macrophytes and pollutants during the process. For this purpose, the seedlings of Phragmites australis and Typha angustifolia were planted respectively in the VF CWs added with HCB at a dose of 2 mg/kg. During 96 days of cultivation, we monitored hexachlorobenzene (HCB) removal efficiency by GC/MS and the structure of the rhizosphere bacterial communities in the different VF CWs by denaturing gradient gel electrophoresis (DGGE), and constructed bacterial clone library based on PCR-amplified 16S rRNA gene. As expected, the rhizosphere bacterial communities also remained insensitive to HCB exposure in the wetland soil. The diversity of these microbes presented two stages, from the varied up and down to equilibrium in the entire experimental period. Molecular analysis revealed that the phylum Firmicutes dominated over the bacterial communities. The genera that increased under HCB stress included the well-known HCB-degrading bacteria (Pseudomonas sp. and Alcaligenes sp.) and other common bacteria found in contaminated soil but with lesser known practical functions (Burkholderia sp., Lysinibacillus fusiformis, and Bacillus cereus). Furthermore, there was a certain variance in the relative abundances of the bacterial phyla and HCB removal efficiency among different VF CW treatments. The degradation of HCB in T. angustifolia microcosms was faster than that in P. australis and unvegetated wetlands, and the highest bacterial diversity and richness was found in the VF CWs comprising T. angustifolia.

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References

  • Arroyo P, Ansola G, Miera LES (2013) Effects of substrate, vegetation and flow on arsenic and zinc removal efficiency and microbial diversity in constructed wetlands. Ecol Eng 51:95–103

    Article  Google Scholar 

  • Ávila C, Bayona JM, Martín I, Salas JJ, García J (2015) Emerging organic contaminant removal in a full-scale hybrid constructed wetland system for wastewater treatment and reuse. Ecol Eng 80:108–116

    Article  Google Scholar 

  • Barbera JL, Sweetman AJ, Wijk D, Jones KC (2005) Hexachlorobenzene in the global environment: emissions, levels, distribution, trends and processes. Sci Total Environ 349:1–44

    Article  Google Scholar 

  • Berg G, Smalla K (2009) Plant species and soil type cooperatively shape the structure and function of microbial communities in the rhizosphere. FEMS 68:1–13

    CAS  Google Scholar 

  • Bernard L, Mougel C, Maron PA, Nowak V, Leveque J, Henault C, Haichar FZ, Berge O, Marol C, Balesdent J, Gibiat F, Lemanceau P, Ranjard L (2007) Dynamics and identification of soil microbial populations actively assimilating carbon from C-13-labelled wheat residue as estimated by DNA- and RNA-SIP techniques. Environ Microbiol 9:752–764

    Article  CAS  Google Scholar 

  • Bertin C, Yang XH, Weston LA (2003) The role of root exudates and allelochemicals in the rhizosphere. Plant Soil 256:67–83

    Article  CAS  Google Scholar 

  • Bulgarelli D, Schlaeppi K, Spaepen S, Themaat EVL, Schulze-Lefert P (2013) Structure and functions of the bacterial microbiota of plants. Annu Rev Plant Biol 64:807–838

    Article  CAS  Google Scholar 

  • Chalouati H, Boutet E, Metais B, Fouche E, Sâada MMB, Gamet-Payrastre L (2015) DNA damage and oxidative stress induced at low doses by the fungicide hexachlorobenzene in human intestinal Caco-2 cells. Toxicol Mech Method 6(25):448–458

    Google Scholar 

  • Crouzet O, Poly F, Bonnemoy F, Bru D, Batisson I, Bohatier J, Philippot L, Mallet C (2015) Functional and structural responses of soil N-cycling microbial communities to the herbicide mesotrione: a dose-effect microcosm approach. Environ Sci Pollut Res 6:1–11

    Google Scholar 

  • Dankwardt A, Hock B (2001) Immunolocalization of nonextractable (bound) residues of pesticides and industrial contaminants in plants and soil. Chemosphere 45:523–533

    Article  CAS  Google Scholar 

  • Fierer N, Bradford MA, Jackson RB (2007) Toward an ecological classification of soil bacteria. Ecology 88:1354–1364

    Article  Google Scholar 

  • Goldfarb KC, Karaoz U, Hanson CA, Santee CA, Bradford MA, Treseder KK, Wallenstein MD, Brodie EL (2011) Differential growth responses of soil bacterial taxa to carbon substrates of varying chemical recalcitrance. Front Microbiol 2:94–100

    Article  Google Scholar 

  • Goswami MR, Pati UK, Chowdhury A, Mukhopadhyay A (2013) Studies on the effect of cypermethrin on soil microbial biomass and its activity in an alluvial soil. Inter J Agric Food Sci 3:1–9

    Google Scholar 

  • Gundi VAKB, Narasimha G, Reddy BR (2005) Interaction effects of insecticides on microbial populations and dehydrogenase activity in a black clay soil. J Environ Sci Health B 40:269–283

    Article  Google Scholar 

  • Kelly JJ, Häggblom M, Tate RL (2003) Effects of heavy metal contamination and remediation on soil microbial communities in the vicinity of a zinc smelter as indicated by analysis of microbial community phospholipid fatty acid profiles. Biol Fert Soils 38:65–71

    Article  CAS  Google Scholar 

  • Li XZ, Rui JP, Mao YJ, Yannarell A, Mackie R (2014) Dynamics of the bacterial community structure in the rhizosphere of a maize cultivar. Soil Biol Biochem 68:392–401

    Article  CAS  Google Scholar 

  • Liu T, Chen ZL, Shen YF (2009) Aerobic biodegradation of hexachlorobenzene by an acclimated microbial community. Int J Environ Pollut 37:235–244

    Article  Google Scholar 

  • Lovecka P, Pacovska I, Stursa P, Vrchotova B, Kochankova L, Demnerova K (2015) Organochlorinated pesticide degrading microorganisms isolated from contaminated soil. New Biotechnol 32:26–31

    Article  CAS  Google Scholar 

  • Ma X, Havelka MM (2009) Phytotoxicity of chlorinated benzenes to Typha angustifolia and Phragmites communis. Environ Toxicol 24:43–48

    Article  CAS  Google Scholar 

  • Merlin C, Devers M, Béguet J, Boggio B, Rouard N, Martin-Laurent F (2015) Evaluation of the ecotoxicological impact of the organochlorine chlordecone on soil microbial community structure, abundance, and function. Environ Sci Pollut Res 3:1–14

    Google Scholar 

  • Naidu MP, Prasanna T (2013) MICs of cypermethrin, chlorpyriphos and monochrotophos pesticides sprayed in agricultural fields of Coccinia grandis on soil microbes. Curr Res Microbiol Biotechnol 1:12–15

    Google Scholar 

  • Òvreas L, Forney L, Daae FL, Torsvik V (1997) Distribution of bacterioplankton in meromictic lake Saelevannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA. Appl Environ Microbiol 63:3367–3373

    Google Scholar 

  • Philippot L, Raaijmakers JM, Lemanceau P, Putten WH (2013) Going back to the roots: the microbial ecology of the rhizosphere. Nat Rev Microbiol 1–11

  • Piotrowska-Seget Z, Cycon M, Kozdroj J (2005) Metaltolerant bacteria occurring in heavily polluted soil and mine spoil. Appl Soil Ecol 28:237–246

    Article  Google Scholar 

  • Ravenschlag K, Sahm K, Pernthaler J, Amann R (1999) High bacterial diversity in permanently cold marine sediments. Appl Environ Microbiol 65(9):3982–3989

    CAS  Google Scholar 

  • Schloss PD, Handelsman JA (2005) Computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 71:1501–1506

    Article  CAS  Google Scholar 

  • Stottmeister U, Wießner A, Kuschk P, Kappelmeyer U, Kästner M, Bederski O, Müller RA, Moormann H (2003) Effects of plants and microorganisms in constructed wetlands for wastewater treatment. Biotechnol Adv 22:93–117

    Article  CAS  Google Scholar 

  • Sunil S, Rashi G, Shilpi S (2015) Effects of chemical and biological pesticides on plant growth parameters and rhizospheric bacterial community structure in Vigna radiata. J Hazard Mater 291:102–110

    Article  Google Scholar 

  • Takagi K, Iwasaki A, Kamei I, Satsuma K, Yoshioka Y, Harada N (2009) Aerobic mineralization of hexachlorobenzene by newly isolated pentachloronitrobenzene-degrading Nocardioides sp. strain PD653. Appl Environ Microbiol 75:4452–4458

    Article  CAS  Google Scholar 

  • Tejada M, García C, Hernández T, Gómez I (2015) Response of soil microbial activity and biodiversity in soils polluted with different concentrations of cypermethrin insecticide. Arch Environ Contam Toxicol 12:1–12

    Google Scholar 

  • Uhlik O, Strejcek M, Vondracek J, Musilova L, Ridl J, Lovecka P, Maceka T (2014) Bacterial acquisition of hexachlorobenzene-derived carbon in contaminated soil. Chemosphere 113:141–145

    Article  CAS  Google Scholar 

  • Van Eerd LL, Hoagland RE, Zablotowicz RM, Hall JC (2003) Pesticide metabolism in plants and microorganisms. Weed Sci 51:472–495

    Article  CAS  Google Scholar 

  • Viñas M, Sabaté J, Espuny MJ, Solanas AM (2005) Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote- contaminated soil. Appl Environ Microbiol 11(71):7008–7018

    Article  Google Scholar 

  • Vodovnik M, Bistan M, Zorec M, Logar RM (2012) Membrane changes associated with exposure of pseudomonas putida to selected environmental pollutants and their possible roles in toxicity. Acta Chim Slov 59:83–88

    CAS  Google Scholar 

  • Vymazal J (2011) Constructed wetlands for wastewater treatment: five decades of experience. Environ Sci Technol 45:61–69

    Article  CAS  Google Scholar 

  • Vymazal J, Březinová T (2016) Accumulation of heavy metals in aboveground biomass of Phragmites australis in horizontal flow constructed wetlands for wastewater treatment: a review. Chem Eng J 290:232–242

    Article  CAS  Google Scholar 

  • Wang G, Lu YL, Han JY, Luo W, Shi YJ, Wang TY, Sun YM (2010) Hexachlorobenzene sources, levels and human exposure in the environment of China. Environ Int 36:122–130

    Article  Google Scholar 

  • Wang B, Zhang CP, Li SY, Lu GQ, Lu GL, Li S, Zhou YQ (2016) An approach to biodegradation of chlorobenzenes: combination of and bacterial effects on hexachlorobenzene degradation in water. doi: 10.2166/wst.2016.313

  • Yang CH, Crowley DE (2000) Rhizosphere microbial community structure in relation to root location and plant iron nutritional status. Appl Environ Microbiol 66:345–351

    Article  CAS  Google Scholar 

  • Zhou YQ, Li XZ, Li SY, Tang YY, Xin ZJ, Jia Y (2011) A review on soil microbial community in different type constructed wetlands. Chinese J Ecol 30(6):1251–1257

    Google Scholar 

  • Zhou YQ, Trestip S, Li XZ, Truu M, Truu J, Mander Ü (2012) Dechlorination of hexachlorobenzene in treatment microcosm wetlands. Ecol Eng 42:249–255

    Article  Google Scholar 

  • Zhou YQ, Tigane T, Li XZ, Truu M, Truu J, Mander Ü (2013) Hexachlorobenzene dechlorination in constructed wetland mesocosms. Water Res 47:102–110

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the National Natural Science Foundation of China (grant no. 31460144, 41471149, 41501508 & 31670472) and the Key Projects in Yunnan Province Department of Education (grant no. 2014Z148). The constructive comments and suggestions on an earlier draft of this paper by the editor and anonymous reviewers are also appreciated.

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Authors and Affiliations

  1. Research Center for Pollution Control and Ecological Restoration, Yuxi Normal University, Yuxi, 653100, People’s Republic of China

    Cuiping Zhang, Bei Wang, Shuying Li, Guangqiu Lu & Yuanqing Zhou

  2. Institute of Ecology and Geobotany, Yunnan University, Kunming, 650091, People’s Republic of China

    Bei Wang

  3. Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, China

    Xiaoyan Dai

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  1. Cuiping Zhang
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  2. Bei Wang
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  3. Xiaoyan Dai
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  4. Shuying Li
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  5. Guangqiu Lu
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  6. Yuanqing Zhou
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Correspondence to Yuanqing Zhou.

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Responsible editor: Robert Duran

Cuiping Zhang and Bei Wang contributed equally to this work

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Zhang, C., Wang, B., Dai, X. et al. Structure and function of the bacterial communities during rhizoremediation of hexachlorobenzene in constructed wetlands. Environ Sci Pollut Res 24, 11483–11492 (2017). https://doi.org/10.1007/s11356-017-8463-1

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  • DOI: https://doi.org/10.1007/s11356-017-8463-1

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