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Rapid biosynthesized AgNPs from Gelidiella acerosa aqueous extract mitigates quorum sensing mediated biofilm formation of Vibrio species—an in vitro and in vivo approach

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Abstract

The present study explores the non-bactericidal anti-virulence efficacy of green synthesized silver nanoparticles (AgNPs) from Gelidiella acerosa against multi-drug resistant Vibrio spp. Spectral characterization of AgNPs was performed through UV-Visible, FT-IR, and energy-dispersive spectroscopic techniques followed by X-ray crystallography and zeta potential analysis. Further, the structural characterization was done by electron and atomic force microscopic techniques. AgNPs profoundly quelled the quorum sensing mediated violacein production in Chromobacterium violaceum and CV026. Characterized AgNPs at 100 μg mL−1 concentrations depicted a phenomenal anti-biofilm efficacy against Vibrio parahaemolyticus (71%) and Vibrio vulnificus (83%) biofilms, which was further confirmed through light, confocal, and scanning electron microscopic analyses. In vitro bioassays revealed the remarkable inhibitory values of AgNPs, by inhibiting the exopolysaccharide production, hydrophobicity, and motility. In vivo studies using Artemia franciscana larvae also confirmed the anti-infective proficiency, as the AgNPs effectively reduced the bacterial colonization and enhanced the survival rate of larvae up to 100% without any toxicity effect.

Rapid biosynthesized AgNPs from Gelidiella acerosa quench quorum sensing controlled virulence traits in vibrios

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Acknowledgements

The author L. Satish sincerely thanks the University Grants Commission, New Delhi, Government of India for financial support in the form of UGC BSR SRF (UGC order no. F.4-1/2006 (BSR)/7-326/2011/BSR, dated 25 February 2013). The author S. Santhakumari gratefully acknowledges the Department of Biotechnology, Government of India (Grant No. BT/PR4815/AAQ/3/587/2012), for the financial support in the form of DBT-SRF. Also, the authors gratefully acknowledge the computational and bioinformatics facility provided by the Alagappa University Bioinformatics Infrastructure Facility (funded by Department of Biotechnology, Government of India; Grant No. BT/BI/25/001/2006). The funders had no role in experimental design, data collection and analysis, writing of the manuscript, or decision to publish this work.

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  1. L. Satish, S. Santhakumari, and S. Gowrishankar contributed equally for this work.

Authors and Affiliations

  1. Department of Biotechnology, Science Campus, Alagappa University, Karaikudi, Tamil Nadu, 630 003, India

    Lakkakula Satish, Sivasubramanian Santhakumari, Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian, Arumugam Veera Ravi & Manikandan Ramesh

  2. Department of Biotechnology Engineering, Ben-Gurion University of Nagev, Beer Sheva, 84105, Israel

    Lakkakula Satish

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  1. Lakkakula Satish
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Satish, L., Santhakumari, S., Gowrishankar, S. et al. Rapid biosynthesized AgNPs from Gelidiella acerosa aqueous extract mitigates quorum sensing mediated biofilm formation of Vibrio species—an in vitro and in vivo approach. Environ Sci Pollut Res 24, 27254–27268 (2017). https://doi.org/10.1007/s11356-017-0296-4

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