Abstract
Increasingly, epidemiological evidences indicate chemosynthetic perfluorooctanoic acid (PFOA), an environmental pollutant, induces potential adverse effect on human health after long-term exposure. However, less study has been performed for assessment of acute effect of PFOA exposure on metabolic homeostasis. In experimental designs, PFOA-exposed liver cells in vivo and in vitro were used to discuss underlying mechanism related to PFOA-induced metabolic dysfunction. In serological tests, PFOA-exposed mice showed increased treads of liver functional enzymes in alanine transaminase (ALT), aspartate transaminase (AST), and total bilirubin (T-BIL), trypsinase, low density lipoprotein-cholesterol (LDL-C), and insulin, while blood glucose, high density lipoprotein-cholesterol (HDL-C), and glucagon levels were reduced. In histocytological observations, PFOA-exposed liver showed visible cytoplasmic vesicles, and intact pancreatic islets were observed in PFOA-exposed pancreas. Additionally, increased insulin-positive cells and reduced glucagon-positive cells were detected in PFOA-exposed islets. As shown in immunoassays, PFOA-exposed liver resulted in elevations of cluster of differentiation 36 (CD36)-labeled cells and CD36 protein. In mouse liver cell study, PFOA-exposed cells showed increased cell apoptotic count, and increased phosphorylated levels of Bcl-2 and Bad in the cells. Furthermore, PFOA-exposed liver cells exhibited elevations of CD36-labeled cells and CD36 protein. Taken together, the present data demonstrate that acute exposure to PFOA-impaired liver function is associated with inducting CD36 expression and apoptosis, as well as disrupting key hormones in the pancreas.
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References
Abdel-Misih SR, Bloomston M (2010) Liver anatomy. Surg Clin North Am 90:643–653
Das KP, Wood CR, Lin MT, Starkov AA, Lau C, Wallace KB, Corton JC, Abbott BD (2017) Perfluoroalkyl acids-induced liver steatosis: effects on genes controlling lipid homeostasis. Toxicology 378:37–52
Fujitani Y (2017) Transcriptional regulation of pancreas development and β-cell function [review]. Endocr J 64:477–486
Glatz JF, Luiken JJ (2017) From fat to FAT (CD36/SR-B2): understanding the regulation of cellular fatty acid uptake. Biochimie 136:21–26
Guo C, Xie G, Su M, Wu X, Lu X, Wu K, Wei C (2016) Characterization of acetaminophen-induced cytotoxicity in target tissues. Am J Transl Res 8:4440–4445
Jian JM, Guo Y, Zeng L, Liang-Ying L, Lu X, Wang F, Zeng EY (2017) Global distribution of perfluorochemicals (PFCs) in potential human exposure source—a review. Environ Int 108:51–62
Jones BJ, Tan T, Bloom SR (2012) Minireview: glucagon in stress and energy homeostasis. Endocrinology 153:1049–1054
Klaunig JE, Hocevar BA, Kamendulis LM (2012) Mode of action analysis of perfluorooctanoic acid (PFOA) tumorigenicity and human relevance. Reprod Toxicol 33:410–418
Li K, Gao P, Xiang P, Zhang X, Cui X, Ma LQ (2017a) Molecular mechanisms of PFOA-induced toxicity in animals and humans: implications for health risks. Environ Int 99:43–54
Li R, Guo C, Wu X, Huang Z, Chen J (2017b) FGF21 functions as a sensitive biomarker of APAP-treated patients and mice. Oncotarget 8:44440–44446
Li R, Liang L, Wu X, Ma X, Su M (2017c) Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. Clin Chim Acta 468:1–4
Sheng N, Li J, Liu H, Zhang A, Dai J (2016) Interaction of perfluoroalkyl acids with human liver fatty acid-binding protein. Arch Toxicol 90:217–227
Sonksen P, Sonksen J (2000) Insulin: understanding its action in health and disease. Br J Anaesth 85:69–79
Su M, Chao G, Liang M, Song J, Wu K (2016) Anticytoproliferative effect of vitamin C on rat hepatic stellate cell. Am J Transl Res 8:2820–2825
Vernon RG (2005) Lipid metabolism during lactation: a review of adipose tissue-liver interactions and the development of fatty liver. J Dairy Res 72:460–469
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This study is granted in part by a funding from National Natural Science Foundation of Guangxi (No. 2016GXNSFBA380055).
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Wu, X., Liang, M., Yang, Z. et al. Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro. Environ Sci Pollut Res 24, 24201–24206 (2017). https://doi.org/10.1007/s11356-017-0072-5
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DOI: https://doi.org/10.1007/s11356-017-0072-5