Abstract
Phosphorus (P) in agricultural ecosystems is an essential and limited element for plants and microorganisms. However, environmental problems caused by P accumulation as well as by P loss have become more and more serious. Oxygen isotopes of phosphate can trace the sources, migration, and transformation of P in agricultural soils. In order to use the isotopes of phosphate oxygen, appropriate extraction and purification methods for inorganic phosphate from soils are necessary. Here, we combined two different methods to analyze the oxygen isotopic composition of inorganic phosphate (δ18OP) from chemical fertilizers and different fractions (Milli-Q water, 0.5 mol L−1 NaHCO3 (pH = 8.5), 0.1 mol L−1 NaOH and 1 mol L−1 HCl) of agricultural soils from the Beijing area. The δ18OP results of the water extracts and NaHCO3 extracts in most samples were close to the calculated equilibrium value. These phenomena can be explained by rapid P cycling in soils and the influence of chemical fertilizers. The δ18OP value of the water extracts and NaHCO3 extracts in some soil samples below the equilibrium value may be caused by the hydrolysis of organic P fractions mediated by extracellular enzymes. The δ18OP values of the NaOH extracts were above the calculated equilibrium value reflecting the balance state between microbial uptake of phosphate and the release of intracellular phosphate back to the soil. The HCl extracts with the lowest δ18OP values and highest phosphate concentrations indicated that the HCl fraction was affected by microbial activity. Hence, these δ18Op values likely reflected the oxygen isotopic values of the parent materials. The results suggested that phosphate oxygen isotope analyses could be an effective tool in order to trace phosphate sources, transformation processes, and its utilization by microorganisms in agricultural soils.
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References
Abigail P, Andres C (2015) A review on useful concepts for stable isotope of oxygen in phosphate (δ18Op) extraction, purification and analysis of freshwater samples and other potential phosphate sources. Microchem J 123:105–110
Amiot R, Wang X, Lécuyer C, Buffetaut E, Boudad L, Cavin L, Ding ZL, Fluteau F, Kellner AWA, Tong HY, Zhang FS (2010a) Oxygen and carbon isotope compositions of middle cretaceous vertebrates from North Africa and Brazil: ecological and environmental significance. Palaeogeogr Palaeocl 297:439–451
Amiot R, Wang X, Zhou ZH, Wang XL, Buffetaut E, Lécuyer C, Ding ZL, Fluteau F, Hibino T, Kusuhashi N, Mo JY, Suteethorn V, Wang YQ, Xu X, Zhang FS (2010b) Oxygen isotopes of east Asian dinosaurs reveal exceptionally cold early cretaceous climates. Proc Natl Acad Sci U S A 108:5179–5183
Angert A, Weiner T, Mazeh S, Tamburini F, Frossard E, Bernasconi SM, Sternberg M (2011) Seasonal variability of soil phosphate stable oxygen isotopes in rainfall manipulation experiments. Geochim Cosmochim Acta 75:4216–4227
Angert A, Weiner T, Mazeh S, Sternberg M (2012) Soil phosphate stable oxygen isotopes across rainfall and bedrock gradients. Environ Sci Technol 46:2156–2162
Blake RE, O’Neil JR, Gracia GA (1997) Oxygen isotope systematics of biologically mediated reactions of phosphate: I. Microbial degradation of organophosphorus compounds. Geochim Cosmochim Acta 61:4411–4422
Blake RE, O’Neil JR, Surkov AV (2005) Biogeochemical cycling of phosphorus: insights from oxygen isotope effects of phosphoenzymes. Am J Sci 305:596–620
Blake RE, Chang SJ, Lepland A (2010) Phosphate oxygen isotopic evidence for a temperate and biologically active Archaean ocean. Nature 46:1029–1033
Colman AS, Blake RE, Karl DM, Fogel ML, Turekian KK (2005) Marine phosphate oxygen isotopes and organic matter remineralization in the oceans. Proc Natl Acad Sci U S A 102:13023–13028
Ding H, Cai GX, Wang YS, Cheng DL (2001) Nitrification and denitrification potential in different types of soils in the North China Plain. Agro-environmental Protection 20:390–393 in Chinese
Elsbury K, Paytan A, Ostrom NE, Kendall C, Young MB, Rollog ME, Watson AS (2009) Using oxygen isotopes of phosphate to trace phosphorus sources and cycling in Lake Erie. Environ Sci Technol 43:3108–3114
Fu BJ, Ma KM, Zhou HF, Chen LD (1999) The effect of land use structure on the distribution of soil nutrients in the hilly area of the Loess Plateau, China. Chinese Sci Bull 44:732–736
Gao SJ, XD D, Li L, Wang CC, Li JQ (2015) Soil inorganic phosphorus speciation analysis of Beijing agricultural soils. Environ Chem 34:586–588 in Chinese
Geohring LD, McHugh OV, Walter MT, Steenhuis TS, Akhtar MS, Walter MF (2001) Phosphorus transport into subsurface drains by macropores after manure applications: implications for best manure management practices. Soil Sci 166:896–909
Gross A, Angert A (2015a) What processes control the oxygen isotopes of soil bio-available phosphate? Geochim Cosmochim Acta 159:100–111
Gross A, Turner BL, Wright SJ, Tanner EVJ, Reichstein M, Weiner T, Angert A (2015b) Oxygen isotope ratios of plant available phosphate in lowland tropical forest soils. Soil Biol Biochem 88:354–361
Gruau G, Legeas M, Riou C, Gallacier E, Martineau FO, Hénin O (2005) The oxygen isotope composition of dissolved anthropogenic phosphates: a new tool for eutrophication research. Water Res 39:232–238
Heckrath G, Brookes PC, Poulton PR, Goulding KWT (1995) Phosphorus leaching from soils containing different phosphorus concentrations in the Broadbalk experiment. J Environ Qua 24:904–910
Hiradate S, Yonezawa T, Takesako H (2006) Isolation and purification of hydrophilic fulvic acids by precipitation. Geoderma 132:196–205
Jaisi DP (2013) Stable isotope fractionations during reactive transport of phosphate in packed-bed sediment columns. J Contam Hydrol 154:10–19
Jaisi DP, Blake RE (2010) Tracing sources and cycling of phosphorus in Peru margin sediments using oxygen isotopes in authigenic and detrital phosphates. Geochim Cosmochim Acta 74:3199–3212
Jaisi DP, Kukkadapu RK, Stout LM, Varga T, Blake RE (2011) Biotic and abiotic pathways of phosphorus cycling in minerals and sediments: insights from oxygen isotope ratios in phosphate. Environ Sci Technol 45:6254–6261
Kolodny Y, Luz B, Navon O (1983) Oxygen isotope variations in phosphate of biogenic apatites, I. Fish bone apatite-rechecking the rules of the game. Earth Planet Sci Lett 64:398–404
Lecuyer C, Grandjean P, Emig CC (1996) Determination of oxygen isotope fractionation between water and phosphate from living lingulids: potential application to paleoenvironmental studies. Palaeogeogr Palaeocl 126:101–108
Li X, Wang Y, Stern J, BH G (2011) Isotopic evidence for the source and fate of phosphorus in Everglades wetland ecosystems. Appl Geochem 26:688–695
Liang Y, Blake R (2006a) Oxygen isotope composition of phosphate in organic compounds: isotope effects of extraction methods. Org Geochem 37:1263–1277
Liang Y, Blake R (2006b) Oxygen isotope signature of pi regeneration from organic compounds by phosphomonoesterases and photooxidation. Geochim Cosmochim Acta 70:3957–3969
Liang Y, Blake RE (2009) Compound-and enzyme-specific phosphodiester hydrolysis mechanisms revealed by δ 18O of dissolved inorganic phosphate. Implications for marine P cycling. Geochim Cosmochim Acta 73:3782–3794
Longinelli A, Nuti S (1973) Oxygen isotope measurements of phosphate from fish teeth and bones. Earth Planet Sci Lett 19:373–376
Ma LF (2002) Geological atlas of China. Geology Publishing House, Beijing in Chinese
Mahowald N, Jickells TD, Baker AR, Artaxo P, Benitez-Nelson CR, Bergametti G, Bond TC, Chen Y, Cohen DD, Herut B, Kubilay N, Losno R, Luo C, Maenhaut W, McGee KA, Okin GS, Siefert R, Tsukuda S (2008) Global distribution of atmospheric phosphorus sources, concentrations and deposition rates, and anthropogenic impacts. Glob Biogeochem Cycles 22:37–42
McLaughlin K, Silva SR, Kendall C, Stuart-Williams H, Paytan A (2004) A precise method for the analysis of δ18O of dissolved inorganic phosphate in seawater. Limnol Oceanogr Meth 2:202–212
McLaughlin K, Chavez F, Pennington JT, Paytan A (2006) A time series investigation of the oxygen isotope composition of dissolved inorganic phosphate in Monterey Bay, California. Limnol Oceanogr Meth 51:2370–2379
Melby ES, Soldat DJ, Barak P (2013) Biological decay of 18O-labeled phosphate in soils. Soil Biol Biochem 63:124–128
O’Neil JR, Vennemann TW, McKenzie WF (2003) Effects of speciation on equilibrium fractionations and rates of oxygen isotope exchange between (PO4)aq and H2O. Geochim Cosmochim Acta 67:3135–3144
Okin GS, Mahowald N, Chadwick OA, Artaxo P (2004) Impact of desert dust on the biogeochemistry of phosphorus in terrestrial ecosystems. Glob Biogeochem Cycles 18:649–655
Paytan A, Kolodny Y, Neori A, Luz B (2002) Rapid biologically mediated oxygen isotope exchange between water and phosphorus. Glob Biogeochem Cycles 16:1–7
Roberts K, Defforey D, Turner BL, Condron LM, Peek S, Silva S, Kendall C, Paytan A (2015) Oxygen isotopes of phosphate and soil phosphorus cycling across a 6500 year chronosequence under lowland temperate rainforest. Geoderma 257–258:14–21
Stout LM, Joshi SR, Kana TM, Jaisi DP (2014) Microbial activities and phosphorus cycling: an application of oxygen isotope ratios in phosphate. Geochim Cosmochim Acta 138:101–116
Tamburini F, Bernasconi SM, Angert A, Weiner T, Frossard E (2010) A method for the analysis of the δ18O of inorganic phosphate extracted from soils with HCl. Eur J Soil Sci 61:1025–1032
Tamburini F, Pfahler FV, Bünemann EK, Guelland K, Bernasconi SM, Frossard E (2012) Oxygen isotopes unravel the role of microorganisms in phosphate cycling in soils. Environ Sci Technol 43:5956–5962
Tamburini F, Pfahler V, von Sperber C, Frossard E, Bernasconi SM (2014) Oxygen isotopes for unraveling phosphorus transformations in the soil-plant system: a review. Soil Sci Soc Am J 78:38–46
von Sperber C, Kries H, Tamburini F, Bernascono SM, Frossard E (2014) The effect of phosphomonoesterases on the oxygen isotope composition of phosphate. Geochim Cosmochim Acta 125:519–527
Wang SY, KL H, Lu P, TQ Y (2009) Spatial variability of soil available phosphorus and environmental risk analysis of soil phosphorus in Pinggu County of Beijing. Sci Agric Sin 42:1290–1298 in Chinese
Young MB, McLaughlin K, Kendall C, Stringfellow W, Rollog M, Elsbury K, Donald E, Paytan A (2009) Characterizing the oxygen isotopic composition of phosphate sources to aquatic ecosystems. Environ Sci Technol 43:5190–5196
Zhang H, Wang JN, Zhu YG, Zhang X (2015) Research and application of analytical technique on δ18Op of inorganic phosphate in soil. Chin J Anal Chem 43:187–192
Zhao XL, Xia XH (2012) Total nitrogen and total phosphorous in urban soils used for different purposes in Beijing, China. Procedia Environ Sci 13:95–104
Zohar I, Shaviv A, Klass T, Paytan A (2010a) Method for the analysis of oxygen isotopic composition of soil phosphate fractions. Environ Sci Technol 44:7583–7588
Zohar I, Shaviv A, Young M, Kendall C, Silva S, Paytan A (2010b) Phosphorus dynamics in soils irrigated with reclaimed waste water or fresh water—a study using oxygen isotopic composition of phosphate. Geoderma 159:109–121
Acknowledgments
We thank Wenjun Huang instrumental support of the XRD spectrum analysis at the Institute of Urban Environment, Chinese Academy of Sciences and Wang Xu instruction in the process of experiment at Institute of Geology and Geophysics, Chinese Academy of Sciences. This work was financially supported by Project of Chinese Academy of Sciences (No. XDB15020401).
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Tian, L., Guo, Q., Zhu, Y. et al. Research and application of method of oxygen isotope of inorganic phosphate in Beijing agricultural soils. Environ Sci Pollut Res 23, 23406–23414 (2016). https://doi.org/10.1007/s11356-016-7482-7
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DOI: https://doi.org/10.1007/s11356-016-7482-7