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Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing

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Abstract

The characterization of bacterial community compositions and the change in perfluoroalkyl acids (PFAAs) along a natural river distribution system were explored in the present study. Illumina high-throughput sequencing was used to explore bacterial community diversity and structure in sediment polluted by PFAAs from the Xiaoqing River, the area with concentrated fluorochemical facilities in China. The concentration of PFAAs was in the range of 8.44–465.60 ng/g dry weight (dw) in sediment. Perfluorooctanoic acid (PFOA) was the dominant PFAA in all samples, which accounted for 94.2 % of total PFAAs. High-level PFOA could lead to an obvious increase in relative abundance of Proteobacteria, ε-Proteobacteria, Thiobacillus, and Sulfurimonas and the decrease in relative abundance of other bacteria. Redundancy analysis revealed that PFOA played an important role in the formation of bacterial community, and PFOA at higher concentration could reduce the diversity of bacterial community. When the concentration of PFOA was below 100 ng/g dw in sediment, no significant effect on microbial community structure was observed. Thiobacillus and Sulfurimonas were positively correlated with the concentration of PFOA, suggesting that both genera were resistant to PFOA contamination.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (nos. 41571478 and 41171394), the Key Research Program of the Chinese Academy of Sciences (no. KZZD-EW-TZ-12), and the National Fundamental Field Study Program (no. 2013FY11110).

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Authors and Affiliations

  1. State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    Yajun Sun, Tieyu Wang, Xiawei Peng, Pei Wang & Yonglong Lu

  2. College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

    Yajun Sun & Xiawei Peng

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  1. Yajun Sun
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  2. Tieyu Wang
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Correspondence to Tieyu Wang.

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Sun, Y., Wang, T., Peng, X. et al. Bacterial community compositions in sediment polluted by perfluoroalkyl acids (PFAAs) using Illumina high-throughput sequencing. Environ Sci Pollut Res 23, 10556–10565 (2016). https://doi.org/10.1007/s11356-016-6055-0

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