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Microbial community characteristics during simultaneous nitrification-denitrification process: effect of COD/TP ratio

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Abstract

To evaluate the impact of chemical oxygen demand (COD)/total phosphorus (TP) ratio on microbial community characteristics during low-oxygen simultaneous nitrification and denitrification process, three anaerobic-aeration (low-oxygen) sequencing batch reactors, namely R1, R2, and R3, were performed under three different COD/TP ratios of 91.6, 40.8, and 27.6. The community structures of each reactor were analyzed via molecular biological technique. The results showed that the composition of ammonia-oxidizing bacteria (AOB) was affected, indicated by Shannon indexes of the samples from R1, R2, and R3. Nitrosomonas was identified to be the dominant AOB in all SBRs. Moreover, the copy numbers of nitrifiers were more than those of denitrifiers, and the phosphorus-accumulating organisms to glycogen-accumulating organisms ratio increased with the decrease of COD/TP ratio.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 21177075 and 50908133), Program for New Century Excellent Talents in University (NCET-10-0554), Natural Science Foundation for Distinguished Young Scholars of Shandong province (JQ201216), and Natural Science Foundation of Jiangsu Normal University (No. 13XLR023).

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China

    Jian Zhang, Rong Wang & Shuang Liang

  2. School of Chemistry and Chemical Engineering, Jiangsu Normal University, Xuzhou, 221116, China

    Wenlin Jia

  3. School of Civil and Environmental Engineering, University of Technology Sydney, Broadway, NSW 2007, Australia

    Huu Hao Ngo & Wenshan Guo

  4. Environmental Research Institute, Shandong University, Jinan, 250100, China

    Huijun Xie

Authors
  1. Jian Zhang
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  2. Wenlin Jia
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  3. Rong Wang
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  4. Huu Hao Ngo
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  5. Wenshan Guo
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  6. Huijun Xie
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  7. Shuang Liang
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Correspondence to Shuang Liang.

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Responsible editor: Robert Duran

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Zhang, J., Jia, W., Wang, R. et al. Microbial community characteristics during simultaneous nitrification-denitrification process: effect of COD/TP ratio. Environ Sci Pollut Res 23, 2557–2565 (2016). https://doi.org/10.1007/s11356-015-5496-1

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