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Transcriptional response of stress-regulated genes to industrial effluent exposure in the cockle Cerastoderma glaucum

  • Molecular and cellular effects of contamination in aquatic ecosystems
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Abstract

This study assessed the responses of molecular biomarkers and heavy metal levels in Cerastoderma glaucum exposed for 1 week to two industrial effluents (1 %) discharged into the Tunisian coastal area, F1 and F2, produced by different units of production of a phosphate treatment plant. A significant uptake of metals (Cd, Cu, Zn, and Ni) was observed in exposed cockles compared to controls, with an uptake higher for F1 than for F2. A decrease in LT50 (stress on stress test) was also observed after an exposure to the effluent F1. Treatments resulted in different patterns of messenger RNA (mRNA) expression of the different genes tested in this report. Gene transcription monitoring performed on seven genes potentially involved in the tolerance to metal exposure showed that for both exposures, mechanisms are rapidly and synchronically settled down to prevent damage to cellular components, by (1) handling and exporting out metal ions through the up-regulation of ATP-binding cassette xenobiotic transporter (ABCB1) and metallothionein (MT), (2) increasing the mRNA expression of antioxidant enzymes (catalase (CAT), superoxide dismutases, CuZnSOD and MnSOD), (3) protecting and/or repairing proteins through the expression of heat shock protein 70 (HSP70) mRNAs, and (4) increasing ATP production (through the up-regulation of cytochrome c oxidase 1 (CO1)) to provide energy for cells to tolerate stress exposure. The tools developed may be useful both for future control strategies and for the use of the cockle C. glaucum as a sentinel species.

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Acknowledgments

This research was financially supported by the European Program FP7 PEOPLE-IRSES GENERA (use of genomic and proteomic tools for the development of contaminant specific biomarkers for the environmental risk assessment of aquatic ecosystems). A. Goupille is acknowledged for the ICP-OES measurements.

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Authors and Affiliations

  1. EA 2460 Mer Molécules Santé, Institut Universitaire Mer et Littoral - FR3473 CNRS, Université du Maine - Le Mans, 72085, Le Mans Cedex, France

    Sahar Karray, Emmanuelle Tastard, Brigitte Moreau, Françoise Denis, Benoît Chénais & Justine Marchand

  2. Laboratoire d’Ecotoxicologie Marine et Environnementale, Université de Sfax, Sfax, Tunisia

    Sahar Karray & Amel Hamza-Chaffai

  3. UMR-I 02 Stress Environnementaux et Biosurveillance des Milieux aquatiques (SEBIO), Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 2, France

    Laurence Delahaut & Alain Geffard

  4. UMR 7312 CNRS-URCA Institut de Chimie Moléculaire de Reims (ICMR) Groupe Chimie de Coordination, Université de Reims Champagne-Ardenne, Moulin de la Housse, BP 1039, 51687, Reims Cedex 2, France

    Emmanuel Guillon

  5. UMR 7208 CNRS-MNHN-IRD-UPMC, Biologie des Organismes et des Ecosystèmes Aquatiques (BOREA), Concarneau, France

    Françoise Denis

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  1. Sahar Karray
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  2. Emmanuelle Tastard
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Correspondence to Justine Marchand.

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Karray, S., Tastard, E., Moreau, B. et al. Transcriptional response of stress-regulated genes to industrial effluent exposure in the cockle Cerastoderma glaucum . Environ Sci Pollut Res 22, 17303–17316 (2015). https://doi.org/10.1007/s11356-015-4108-4

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