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Effect of plant growth-promoting rhizobacteria inoculation on cadmium (Cd) uptake by Eruca sativa

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Abstract

Microbe-assisted phyto-remediation approach is widely applied and appropriate choice to reduce the environmental risk of heavy metals originated from contaminated soils. The present study was designed to screen out the nested belongings of Eruca sativa plants and Pseudomonas putida (ATCC 39213) at varying cadmium (Cd) levels and their potential to deal with Cd uptake from soils. We carried out pot trial experiment by examining the soil containing E. sativa seedlings either treated with P. putida and/or untreated plants subjected to three different levels (ppm) of Cd (i.e., 150, 250, and 500). In all studied cases, we observed an increase in Cd uptake for E. sativa plants inoculated with P. putida than those of un-inoculated plants. Cd toxicity was assessed by recording different parameters including stunted shoot growth, poor rooting, and Cd residual levels in the plants that were not inoculated with P. putida. Significant difference (p < 0.05) of different growth parameters for inoculated vs non-inoculated plants was observed at all given treatments. However, among the different treatments, E. sativa exhibited increased values for different growth parameters (except proline contents) at lower Cd levels than those of their corresponding higher levels, shoot length (up to 27 %), root length (up to 32 %), whole fresh plant (up to 40 %), dry weight (up to 22 %), and chlorophyll contents (up to 26 %). Despite the hyperaccumulation of Cd in whole plant of E. sativa, P. putida improved the plant growth at varying levels of Cd supply than those of associated non-inoculated plants. Present results indicated that inoculation with P. putida enhanced the Cd uptake potential of E. sativa and favors the healthy growth under Cd stress.

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Acknowledgments

The authors are thankful to the Department of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University Islamabad, Pakistan, for providing funds for transportation during fieldwork and experimental analysis.

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan

    Muhammad Aqeel Kamran, Muhammad Farooq Hussain Munis & Hassan Javed Chaudhary

  2. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China

    Jabir Hussain Syed

  3. Public Health and Environment Division, Department of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan

    Syed Ali Musstjab Akber Shah Eqani

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  1. Muhammad Aqeel Kamran
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  2. Jabir Hussain Syed
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Correspondence to Hassan Javed Chaudhary.

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Kamran, M.A., Syed, J.H., Eqani, S.A.M.A.S. et al. Effect of plant growth-promoting rhizobacteria inoculation on cadmium (Cd) uptake by Eruca sativa . Environ Sci Pollut Res 22, 9275–9283 (2015). https://doi.org/10.1007/s11356-015-4074-x

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