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Metabolomics-based approach for assessing the toxicity mechanisms of dibutyl phthalate to abalone (Haliotis diversicolor supertexta)

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Abstract

Dibutyl phthalate (DBP) is a ubiquitous contaminant in the marine environment, and relatively little is known about the toxicological mechanisms of this compound at the metabolite level. In this study, marine gastropods (abalone) were exposed to DBP at environmentally relevant concentrations (2, 10, and 50 μg/L) for 30 days. The plasma metabolite profiles were determined at the 5th, 15th, and 30th. The major metabolite changes corresponding to DBP exposure were related to osmotic regulation, energy metabolism, and environmental stress, and the effects displayed a dose-dependent pattern. The most obvious change was the increase in the levels of intracellular metabolites (betaine, dimethylglycine, homarine, glutamine, and lactate) and tricarboxylic acid cycle intermediates. The results revealed that DBP may lead to abalone oxidative stress, lipid metabolism dysfunction, energy metabolism disturbance, and osmoregulation imbalance. These results would be helpful in better understanding the mechanisms of abalone response to DBP stress at the system level.

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Acknowledgments

This study was supported by Marine Public Welfare Projects of China (201205020-9, 201305021-1), Natural Science Foundation of China (41106087, 41476092), Guangdong Province Marine and Fishery Special Funding of Science & Technology Promote Project (A201303F06), Shenzhen Key Laboratory for Coastal Ocean Dynamic and Environment (ZDSY20130402163735964),Special Fund of State Key Joint Laboratory of Environment Simulation and Pollution Control (13K10ESPCT), Fundamental Research Project of Shenzhen (JCYJ20130402145002375, JCYJ20140417115840286), and Innovation Research & Develop Project of Nanshan District of Shenzhen (KC2013JSCX003A).

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Authors and Affiliations

  1. The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University, Room 304, Building L, Shenzhen University Town, Xili, Shenzhen City, 518055, People’s Republic of China

    Jin Zhou & Zhonghua Cai

  2. Shenzhen Public Platform for Screening and Application of Marine Microbial Resources, Shenzhen City, Guangdong Province, People’s Republic of China

    Jin Zhou & Zhonghua Cai

  3. Department of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen, Guangdong Province, 518055, People’s Republic of China

    Baiyang Chen

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  1. Jin Zhou
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Correspondence to Zhonghua Cai.

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Responsible editor: Philippe Garrigues

Jin Zhou and Baiyang Chen contributed equally to this work.

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Zhou, J., Chen, B. & Cai, Z. Metabolomics-based approach for assessing the toxicity mechanisms of dibutyl phthalate to abalone (Haliotis diversicolor supertexta). Environ Sci Pollut Res 22, 5092–5099 (2015). https://doi.org/10.1007/s11356-014-3859-7

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