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Enzymatic removal of paracetamol from aqueous phase: horseradish peroxidase immobilized on nanofibrous membranes

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Abstract

Paracetamol is a widely used as an analgesic and an antipyretic that can easily accumulate in aquatic environments. This study aimed to enhance paracetamol removal efficiency from water by combining the biocatalytic activity of horseradish peroxidase (HRP) with the adsorption of nanofibrous membrane. Poly(vinyl alcohol)/poly(acrylic acid)/SiO2 electrospinning nanofibrous membrane was prepared with fiber diameters of 200 to 300 nm. The membrane was made insoluble by the thermal cross-linking process. HRP, which was previously activated by 1,1′-carbonyldiimidazole, was covalently immobilized on the surface of nanofibers. Immobilized HRP retained 79.4 % of the activity of free HRP. The physical, chemical, and biochemical properties of the immobilized HRP and its application in paracetamol removal were comprehensively investigated. Immobilized HRP showed better storage capability and higher tolerance to the changes in pH and temperature than free HRP. Paracetamol removal rate by immobilized HRP (83.5 %) was similar to that of free HRP (84.4 %), but immobilized HRP showed excellent reusability. The results signify that enzyme immobilized on nanofibers has great application potential in water treatment.

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Acknowledgments

This study was funded by the National Natural Science Foundation of China (51208368). The authors are also grateful for the financial support from the Ministry of Science and Technology, China (Nos. 2010DFA92820 and 2010DFA92800).

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, China

    Ran Xu, Yifang Si, Fengting Li & Bingru Zhang

  2. College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, People’s Republic of China

    Ran Xu

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  1. Ran Xu
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  2. Yifang Si
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Correspondence to Ran Xu.

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Responsible editor: Angeles Blanco

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Xu, R., Si, Y., Li, F. et al. Enzymatic removal of paracetamol from aqueous phase: horseradish peroxidase immobilized on nanofibrous membranes. Environ Sci Pollut Res 22, 3838–3846 (2015). https://doi.org/10.1007/s11356-014-3658-1

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  • DOI: https://doi.org/10.1007/s11356-014-3658-1

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