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Degradation of polyurethane by bacterium isolated from soil and assessment of polyurethanolytic activity of a Pseudomonas putida strain

  • Biodegradability assessment of organic substances and polymers
  • Published:
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Abstract

The increasing usage and the persistence of polyester polyurethane (PU) generate significant sources of environmental pollution. The effective and environmental friendly bioremediation techniques for this refractory waste are in high demand. In this study, three novel PU degrading bacteria were isolated from farm soils and activated sludge. Based upon 16S ribosomal RNA gene sequence blast, their identities were determined. Particularly robust activity was observed in Pseudomonas putida; it spent 4 days to degrade 92 % of Impranil DLNTM for supporting its growth. The optimum temperature and pH for DLN removal by P. putida were 25 °C and 8.4, respectively. The degradation and transformation of DLN investigated by Fourier transformed infrared spectroscopy show the decrease in ester functional group and the emergence of amide group. The polyurethanolytic activities were both presented in the extracellular fraction and in the cytosol. Esterase activity was detected in the cell lysate. A 45-kDa protein bearing polyurethanolytic activity was also detected in the extracellular medium. This study presented high PU degrading activity of P. putida and demonstrated its responsible enzymes during the PU degradation process, which could be applied in the bioremediation and management of plastic wastes.

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Acknowledgments

The authors thank Bayer Material Science for offering free sample.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 106, Taiwan, China

    Yu-Huei Peng, Yang-hsin Shih, Yen-Chun Lai, Yuan-Zan Liu, Ying-Tong Liu & Nai-Chun Lin

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  1. Yu-Huei Peng
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  2. Yang-hsin Shih
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  3. Yen-Chun Lai
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  4. Yuan-Zan Liu
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Correspondence to Yang-hsin Shih.

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Responsible editor: Robert Duran

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Peng, YH., Shih, Yh., Lai, YC. et al. Degradation of polyurethane by bacterium isolated from soil and assessment of polyurethanolytic activity of a Pseudomonas putida strain. Environ Sci Pollut Res 21, 9529–9537 (2014). https://doi.org/10.1007/s11356-014-2647-8

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  • DOI: https://doi.org/10.1007/s11356-014-2647-8

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