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New insight into molecular interaction of heavy metal pollutant—cadmium(II) with human serum albumin

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An Erratum to this article was published on 12 January 2016

Abstract

Cadmium (Cd) is an extremely toxic metal commonly found as an environmental contaminant from industrial and agricultural sources, posing severe risks to human health. In this study, the binding mechanism of Cd(II)–human serum albumin (HSA) complex and the effect of Cd(II) on the conformational stability and structural state of HSA were comprehensively investigated through a series of efficient and appropriate methods. X-ray photoelectron spectroscopy accurately described the microenvironmental changes around protein C, N, and O atoms in the presence of Cd(II). Fluorescence results indicated that the probable mechanism of Cd(II)–HSA interaction is a static quenching process. Fourier transform infrared spectroscopy and dynamic light scattering showed Cd(II) complexation altered HSA conformation and the microenvironments of Trp and Tyr residues, accompanied by the size increases of HSA aggregates. This research will be helpful for understanding the toxic effects of Cd(II) on protein function in vivo.

The detailed binding mechanism between Cd(II) and HSA accompanied with the conformational analysis of HSA was comprehensively investigated at the molecular level

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Acknowledgments

The authors gratefully acknowledge the financial supports from the National Natural Science Foundation of China (no. 20875055); the Natural Science Foundation of Fujian Province, China (no. 2013J01388); the State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter; and the Opening Research Foundation of Key Laboratory of Biomedical Material in Tianjin city.

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Authors and Affiliations

  1. The State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, People’s Republic of China

    Yan Liu, Longguang Jiang & Ling Song

  2. Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, Fujian, 350002, People’s Republic of China

    Mingmao Chen

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  1. Yan Liu
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  2. Mingmao Chen
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  3. Longguang Jiang
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  4. Ling Song
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Correspondence to Ling Song.

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Responsible editor: Philippe Garrigues

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Liu, Y., Chen, M., Jiang, L. et al. New insight into molecular interaction of heavy metal pollutant—cadmium(II) with human serum albumin. Environ Sci Pollut Res 21, 6994–7005 (2014). https://doi.org/10.1007/s11356-014-2610-8

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  • DOI: https://doi.org/10.1007/s11356-014-2610-8

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