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Larvicidal, ovicidal, and oviposition-deterrent activities of four plant extracts against three mosquito species

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Abstract

In mosquito control programs, insecticides of botanical origin have the potential to eliminate eggs, larvae, and adults. So, the larvicidal, ovicidal, and oviposition-deterrent activities of petroleum ether and ethyl acetate extracts of the leaves of Eugenia jambolana, Solidago canadensis, Euodia ridleyi, and Spilanthes mauritiana were assayed against the three vector mosquito species, namely Anopheles stephensi, Aedes aegypti, and Culex quinquefasciatus. The larval bioassay was conducted following the World Health Organization method. The maximum larval mortality was found with ethyl acetate extract of S. mauritiana against the larvae of A. stephensi, A. aegypti, and C. quinquefasciatus with LC50 values of 11.51, 28.1, 14.10 ppm, respectively. The mean percent hatchability of the ovicidal activity was observed at 48-h post-treatment. The percent hatchability was found to be inversely proportional to the concentration of the extract and directly proportional to the number of eggs. The flower head extract of S. mauritiana gave 100 % mortality followed by E. ridleyi, S. canadensis, and E. jambolana against the eggs of the three mosquito vectors. For oviposition-deterrent effect, out of the five concentrations tested (20, 40, 60, 80, and 100 ppm), the concentration of 100 ppm showed a significant egg laying-deterrent capacity. The oviposition activity index value of E. jambolana, E. ridleyi, S. canadensis, and S. mauritiana against A. aegypti, A. stephensi, C. quinquefasciatus at 100 ppm were −0.71, −0.71, −0.90, −0.93, −0.85, −0.91, −1, −1, −0.71, −0.85, −1, and −1, respectively. These results suggest that the leaf/flower extracts of certain local plants have the potential to be developed as possible eco-friendly means for the control of mosquitoes.

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References

  • Abbott WS (1925) A method of computing of the effectiveness of an insecticide. J Econ Entomol 18:265–267

    CAS  Google Scholar 

  • Aivazi AA, Vijayan VA (2010) Efficacy of Ruta graveolens extract and its synergistic effect with cypermethrin against Anopheles stephensi Liston. J Toxicol Environ Chem 92:893–901

    Article  CAS  Google Scholar 

  • Amer A, Melhorn H (2006) Repellency effect of forty-one essential oils against Aedes, Anopheles and Culex mosquitoes. Parasitol Res 99:478–490

    Article  Google Scholar 

  • Apati P, Szentmihalyi K, Kristo ST, Papp I, Vinkler P, Szake E, Kery A (2003) Herbal remedies of Solidago—correlation of phytochemical characteristics and antioxidative properties. J Pharm Biomed Anal 32(4–5):1045–1053

    Article  CAS  Google Scholar 

  • Aregawi M, Cibulskis R, Otten M, Williams R, Dye C (2008). World malaria report. Geneva: World Health Organization (WHO). 190

  • Baruah RN, Leclercq PA (1993) Characterization of the essential oil from flower heads of Spilanthes acmella. J Essent Oil Res 5:693–695

    Article  CAS  Google Scholar 

  • Bernhard L, Bernhard P, Magnussen P (2003) Management of patients with lymphoedema caused by filariasis in north-eastern Tanzania: alternative approaches. Physiotherapy 89:743–749

    Article  Google Scholar 

  • Bhanuprakash VM, Hosmani V, Balamurugan RK, Singh SD (2007) In vitro antiviral activity of Eugenia jambolana plant extract on buffalopox virus: conventional and QPCR methods. Int J Trop Med 2:3–9

    Google Scholar 

  • Burkill IH (1966) A dictionary of the economic products of the Malay peninsula, vol 2. Governments of Malaysia and Singapore by the Ministry of Agriculture and Co-operatives, Kuala Lumpur

    Google Scholar 

  • Chaturvedi AG, Bhawani PK, Agarwal S, Goel A, Singh GRK (2009) Ulcer healing properties of ethanolic extract of Eugenia jambolana seed in diabetic rats: study on gastric mucosal defensive factors. J Physiol Pharmacol 53:16–24

    Google Scholar 

  • Coria C, Almison W, Valladares G, Caspinella C, Luduena F, Defago M, Palacios S (2008) Larvicide and oviposition deterrent effects of fruit and leaf extracts from Melia azadirachta L. on Aedes aegypti (L.) (Diptera Culicidae). Bioresour Technol 99(8):3066–3070

    Article  CAS  Google Scholar 

  • Elango G, Rahuman AA, Bagavan A, Kamaraj C, Zahir AA, Venkatesan C (2009) Laboratory study on larvicidal activity of indigenous plant extracts against Anopheles subpictus and Culex tritaeniorhynchus. Parasitol Res 104(6):1381–1388

    Article  CAS  Google Scholar 

  • Govindarajan M, Jebanesan A, Pushpanathan T, Samidurai K (2008) Studies on effect of Acalypha indica L. (Euphorbiaceae) leaf extracts on the malarial vector, Anopheles stephensi Liston (Diptera: Culicidae) Parasitol Res 103(3):691–695.

    Google Scholar 

  • Govindarajan M (2009) Bioefficacy of Cassia fistula Linn. (Leguminosae) leaf extract against chikungunya vector, Aedes aegypti (Diptera: Culicidae). Eur Rev Med Pharmacol Sci 13(2):99–103

    CAS  Google Scholar 

  • Govindarajan M, Mathivanan T, Elumalai K, Krishnappa K, Anandan A. (2011). Ovicidal and repellent activity of botanical extracts against Culex quinquefasiatus, Aedes aegypti and Anopheles stephensi, (Diptera: Culicidae). Asian. Pac. J. Trop. Med 1(1):43–48

    Google Scholar 

  • Horace F, Clay JC, Hubbard (1977) The Hawaii garden tropical shrubs. The University Press of Hawaii, Singapore, 287

    Google Scholar 

  • Jantan I, Yalvema MF, Ahmad NW, Jamal JA (2005) Insecticidal activities of the leaf oils of eight Cinnamomum species against Aedes aegypti and Aedes albopictus. Pharm Biol 43:526–532

    Article  Google Scholar 

  • Jayaraj S (1993) Neem in pest control. Paper presented at the world neem conference, 24–28 Feb 1993. Bangalore, India, pp 37–43

  • Kalyanasundaram M, Babu CJ (1982) Biologically active plant extracts as mosquito larvicides. Ind J Med Res 76:102–106

    Google Scholar 

  • Kalyanasundaram M, Das PK (1985) Larvicidal and synergistic activity of plant extracts for mosquito control. Ind J Med Res 82:19–23

    CAS  Google Scholar 

  • Kramer WL, Mulla S (1979) Oviposition attractants and repellents of mosquitoes: oviposition responses of Culex mosquitoes to organic infusions. Environ Entomol 8:1111–1117

    Google Scholar 

  • Madhu SK, Shoukath AK, Vijayan VA (2009) Efficacy of bioactive compounds from Curcuma aromatica against mosquito larvae. Acta Trop 113:7–11

    Article  Google Scholar 

  • Markouk M, Bekkouche K, Larhsini M, Bausaid H, Lazrek HB, Jana M (2001) Evaluation of some Moroccan medicinal plant extracts for larvicidal activity. J Ethano Pharmacol 73:293–297

    Article  Google Scholar 

  • Mehra BK, Hiradhar PK (2002) Cuscuta hyalina Roth. and insect development inhibitor against common house mosquito Culex quinquefasciatus Say. J Environ Biol 23:335–339

    Google Scholar 

  • Mittal PK, Subbarao SK (2003) Prospectus of using herbal products in the control of mosquito vector. ICMR Bull 33:1–10

    Google Scholar 

  • Myung K, Massaugbodji A, Ekone S, Atchade P, Kiki-Fagla V, Kilon AD (1998) Lymphatic filariasis in a hyperendemic region: ten year follow up survey. Am J Trop Med Hyg 59(2):222–226

    CAS  Google Scholar 

  • Oliver-Bever B (1986) Medicinal plants in Tropical West Africa. Cambridge University Press, Cambridge, pp 80–81

    Book  Google Scholar 

  • Pancharoen C, Kulwichit W, Tantawichien T, Thisyakorn U, Thisyakam C (2002) Dengue infection a global concern. J Med Assoc Thail 85:25–33

    Google Scholar 

  • Pavela R (2009) Larvicidal effects of some Euroasiatic plants against Culex quinquefasciatus Say. Larvae (Diptera: Culicidae). Parasitol Res 105:887–892

    Article  Google Scholar 

  • Peng Z, Yamng J, Wang H, Simons (1999) Production and characterization of monoclonal antibodies to two new mosquito Aedes aegypti salivary properties. Insect Biochem Med Biol 29:909–914

    Article  CAS  Google Scholar 

  • Prathibha KP, Raghavendra BS, Vijayan VA (2011) Evaluation of larvicidal effect of Euodia ridleyi, Hoch, leaf extract against three mosquito species at Mysore. Int J Biol Sci Eng 02(01):106–109

    Google Scholar 

  • Pushpalatha E, Muthukrishnan J (1995) Larvicidal activity of a few plant extracts against Culex quinquefasciatus and Anopheles stephensi. Ind J Malar Res 32:14–23

    CAS  Google Scholar 

  • Raghavendra BS, Prathibha KP, Vijayan VA (2011) Larvicidal efficacy of Eugenia jambolana Linn, extract in three mosquito species at Mysore. J Entomol 8(5):491–496

    Article  Google Scholar 

  • Rahuman AA, Venkatesan P, Gopala Krishnan G (2008) Mosquito larvicidal activity of oleic and linoleic acids isolated from Citrullus colocynthis (Linn.). Schrad Parasitol Res 103:1383–1390

    Article  Google Scholar 

  • Rajkumar S, Jebasan A (2009) Larvicidal and oviposition activity of Cassia obtusifolia Linn. (Family: Leguminosae) leaf extract against malarial vector, Anopheles stephensi Liston (Diptera: Cuoicidae). Parasitol Res 104(2):337–340

    Article  CAS  Google Scholar 

  • Rigua-Perez JG, Clark GG, Gubler DJ (1997) Dengue and dengue hemorrhagic fever. Lancet 352:971–977

    Article  Google Scholar 

  • Singh NP, Kumari V, Chauhan D (2003) Mosquito larvicidal properties of the leaf extract of a herbaceous plant, Ocimum canum (Family: Labiatae). J Commun Dis 35(1):43–45

    CAS  Google Scholar 

  • Su T, Mulla S (1998) Ovicidal activity of neem products (azadirachtin) against Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae). J Am Mosq Control Assoc 14(2):204–209

    CAS  Google Scholar 

  • Sukumar K, Perich MJ, Boobar LR (1991) Botanical derivatives in mosquito control: a review. J Am Mosq Control Assoc 7:210–237

    CAS  Google Scholar 

  • Taubitz W, Cramer TP, Kapaun A, Pefeffer M, Drasten C, Dobler G, Burchard GD, Loscher T (2007) Chikungunya fever in travelers: clinical presentation and course. Clin Infect Dis 45(1):e1–e4

    Article  Google Scholar 

  • Tawatsin A, Asavadachanukorn P, Thavara U, Wonngiskongman P, Bansidhi J, Boonruad T, Chavalittumrong P, Soonthomchareonnon N, Komalamisra N, Mulla MS (2006) Repellency of essential oils extracted from plants in Thailand against four mosquito vectors (Diptera: Culicidae) and oviposition deterrent effects against Aedes aegypti (Diptera: Culicidae). Southeast Asia J Trop Med Public Health 37(5):915–931

    CAS  Google Scholar 

  • Vahitha R, Venkatachalam MR, Murugan K, Jebanesan A (2002) Larvicidal efficacy of Pavonia zeylanica L. and Acacia ferruginea D.C. against Culex quinquefasciatus Say. Bioresour Technol 82(2):203–204

    Article  CAS  Google Scholar 

  • World Health Organisation (2005).Guideline for laboratory in field testing of mosquito larvicidesWHO/CDS/WHOPES/GCDPP/2005.13.

  • World Health Organization (2010) Malaria. Factsheet No. 94 Geneva. http://www.who.int/mediacentre/factsheets/fso94/en/. Accessed July 2010

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Acknowledgments

Authors are thankful to Chairman DOS in Zoology, Manasagangothri, Mysore, and to the University of Mysore for the facilities.

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Authors and Affiliations

  1. Department of Studies in Zoology, University of Mysore, Manasagangothri, Mysore, Karnataka, 570006, India

    K. P. Prathibha, B. S. Raghavendra & V. A. Vijayan

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  1. K. P. Prathibha
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  2. B. S. Raghavendra
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  3. V. A. Vijayan
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Correspondence to K. P. Prathibha.

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Prathibha, K.P., Raghavendra, B.S. & Vijayan, V.A. Larvicidal, ovicidal, and oviposition-deterrent activities of four plant extracts against three mosquito species. Environ Sci Pollut Res 21, 6736–6743 (2014). https://doi.org/10.1007/s11356-014-2591-7

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