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Distribution of antibiotic-resistant bacteria in chicken manure and manure-fertilized vegetables

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Abstract

Veterinary manure is an important pollution reservoir of antibiotics and antibiotic-resistant bacteria (ARB). However, little is known of the distribution of ARB in plant endophytic bacteria and the number/types of ARB in chicken manure. In this study, 454-pyrosequencing was used to investigate the distribution and composition of ARBs in chicken manure and fertilized vegetables. The prevalence of ARB in the samples of the chicken manure compost recovered from farms on which amoxicillin, kanamycin, gentamicin, and cephalexin were used was 20.91–65.9 % for ARBs and 8.24–20.63 % simultaneously resistant to two or more antibiotics (multiple antibiotic resistant bacteria (MARB)). Antibiotic-resistant endophytic bacteria were widely detected in celery, pakchoi, and cucumber with the highest rate of resistance to cephalexin. The pyrosequencing indicated that the chicken manure dominantly harbored Firmicutes, Bacteroidetes, Synergistetes, and Proteobacteria and that Bacteroidetes was significantly enhanced in farms utilizing antibiotics. In the total cultivable colonies, 62.58–89.43 % ARBs and 95.29 % MARB were clustered in Bacteroidetes with the dominant species (Myroides ordoratimimus and Spningobacterium spp., respectively) related to human clinical opportunistic pathogens.

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Acknowledgments

The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (NSFC 21277041 and NSFC 21077032) and from Program for Innovative Research Team (in Science and Technology) in University of Henan Province (13IRTSTHN009).

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Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, China

    Qingxiang Yang, Siwei Ren, Tianqi Niu, Yuhui Guo, Shiyue Qi, Xinkuan Han & Dong Liu

  2. Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang, 453007, China

    Qingxiang Yang & Feng Pan

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  1. Qingxiang Yang
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  2. Siwei Ren
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  3. Tianqi Niu
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  4. Yuhui Guo
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  5. Shiyue Qi
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  6. Xinkuan Han
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  7. Dong Liu
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  8. Feng Pan
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Correspondence to Qingxiang Yang.

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Responsible editor: Robert Duran

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Yang, Q., Ren, S., Niu, T. et al. Distribution of antibiotic-resistant bacteria in chicken manure and manure-fertilized vegetables. Environ Sci Pollut Res 21, 1231–1241 (2014). https://doi.org/10.1007/s11356-013-1994-1

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  • DOI: https://doi.org/10.1007/s11356-013-1994-1

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