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Mutagenicity and genotoxicity assessment of industrial wastewaters

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Abstract

The genotoxicity of industrial wastewaters from Jajmau (Kanpur), was carried out by Ames Salmonella/microsome test, DNA repair-defective mutants, and Allium cepa anaphase–telophase test. Test samples showed maximum response with TA98 strain with and without metabolic activation. Amberlite resins concentrated wastewater samples were found to be more mutagenic as compared to those of liquid–liquid extracts (hexane and dichloromethane extracts). The damage in the DNA repair defective mutants in the presence of Amberlite resins concentrated water samples were found to be higher to that of liquid–liquid-extracted water samples at the dose level of 20 μl/ml culture. Among all the mutants, polA exhibited maximum decline with test samples. Mitotic index (MI) of root tip meristematic cells of A. cepa treated with 5, 10, 25, 50, and 100 % (v/v) wastewaters were significantly lower than the control. Complementary to the lower levels of MI, the wastewaters showed higher chromosomal aberration levels in all cases investigated.

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Acknowledgments

FM is thankful to the UGC, New Delhi for financial assistance in the form of Maulana Azad National Fellowship. AM is thankful to the Department of Biotechnology, Government of India, New Delhi, for DBT CREST award during the preparation of the manuscript. The authors are also thankful to Sophisticated Analytical Instrument Facility (SAIF) IIT, Bombay, for carrying out GC-MS analysis.

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  1. Abdul Malik

    Present address: Department of Infectious Diseases, Universitaetsklinikum Freiburg, Hugstetter Straße 55, 79106, Freiburg im Breisgau, Germany

Authors and Affiliations

  1. Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002, India

    Farhana Masood & Abdul Malik

Authors
  1. Farhana Masood
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  2. Abdul Malik
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Correspondence to Abdul Malik.

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Responsible editor: Robert Duran

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Masood, F., Malik, A. Mutagenicity and genotoxicity assessment of industrial wastewaters. Environ Sci Pollut Res 20, 7386–7397 (2013). https://doi.org/10.1007/s11356-013-1756-0

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