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A Portable Fluorescence Camera for Testing Surgical Specimens in the Operating Room: Description and Early Evaluation

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Abstract

Purpose

Clinical translation of novel optical probes requires testing of human specimens ex vivo to ensure efficacy. However, it may be difficult to remove human tissue from the operating room due to regulatory/privacy issues. Therefore, we designed a portable fluorescence camera to test targeted optical imaging probes on human specimens in the operating room.

Procedures

A compact benchtop fluorescence camera was designed and built in-house. A mouse xenograft model of ovarian cancer with an activatable imaging probe based on rhodamine green was used to test the device. Comparison was made to commercially available imaging systems.

Results

The prototype camera produced images comparable to images acquired with commercially available, non-portable imaging systems.

Conclusion

We demonstrate the feasibility of a specimen-based portable fluorescence camera for use in the operating room. Its small size ensures that tissue excised from patients can be tested promptly for fluorescence within the operating room environment, thus expediting the testing of novel imaging probes.

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Acknowledgments

This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Signal Processing and Instrumentation Section, Division of Computational Bioscience, Center of Information Technology, National Institutes of Health, 12 South Dr., Bethesda, MD, 20892-5624, USA

    John W. Kakareka & Thomas J. Pohida

  2. Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 10, Room B3B69, MSC1088, 10 Center Dr., Bethesda, MD, 20892-1088, USA

    Thomas E. McCann, Nobuyuki Kosaka, Makoto Mitsunaga, Peter L. Choyke & Hisataka Kobayashi

  3. Biomedical Engineering and Physical Science Shared Resource, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 13 South Dr., Bethesda, MD, 20892-5766, USA

    Nicole Y. Morgan

Authors
  1. John W. Kakareka
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  2. Thomas E. McCann
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  3. Nobuyuki Kosaka
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  4. Makoto Mitsunaga
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  5. Nicole Y. Morgan
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  6. Thomas J. Pohida
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  7. Peter L. Choyke
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  8. Hisataka Kobayashi
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Corresponding author

Correspondence to Hisataka Kobayashi.

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Kakareka, J.W., McCann, T.E., Kosaka, N. et al. A Portable Fluorescence Camera for Testing Surgical Specimens in the Operating Room: Description and Early Evaluation. Mol Imaging Biol 13, 862–867 (2011). https://doi.org/10.1007/s11307-010-0438-2

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  • DOI: https://doi.org/10.1007/s11307-010-0438-2

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