Abstract
Purpose
To study the feasibility of using 2-deoxy-d-glucose (2-DG)-labeled gold nanoparticle (AuNP-DG) as a computed tomography (CT) contrast agent with tumor targeting capability through in vitro experiments.
Procedures
Gold nanoparticles (AuNP) were fabricated and were conjugated with 2-deoxy-d-glucose. The human alveolar epithelial cancer cell line, A-549, was chosen for the in vitro cellular uptake assay. Two groups of cell samples were incubated with the AuNP-DG and the unlabeled AuNP, respectively. Following the incubation, the cells were washed with sterile PBS to remove the excess gold nanoparticles and spun to cell pellets using a centrifuge. The cell pellets were imaged using a microCT scanner immediately after the centrifugation. The reconstructed CT images were analyzed using a commercial software package.
Results
Significant contrast enhancement in the cell samples incubated with the AuNP-DG with respect to the cell samples incubated with the unlabeled AuNP was observed in multiple CT slices.
Conclusions
Results from this study demonstrate enhanced uptake of 2-DG-labeled gold nanoparticle by cancer cells in vitro and warrant further experiments to study the exact molecular mechanism by which the AuNP-DG is internalized and retained in the tumor cells.
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References
Weissleder R (2006). Science 312:1168–1171
Lee TY (2002) Trends in Biotech 20:S3–S10
Kao CY, Hoffman EA, Beck KC et al (2003) Acad Radiol 10:475–483
Schmiedl UP, Krause W, Leike J et al (1999) Acad Radiol 6:164–169
Fruman SA, Harned RK II, Marcus D et al (1994) Acad Radiol 1:151–153
Vera DR, Mattrey RF (2002) Acad Radiol 9:784–792
Bonvento M, Moore W, Button T et al (2003) Acad Radiol 13:979–985
Miyamoto A, Okimoto H, Shinohara H et al (2006) Eur Radiol 16:1050–1053
Yu SB, Watson AD (1999) Chem Rev 99:2352–2378
Rabin O, Manuel Perez J, Grimm J, Wojtkiewicz G, Weissleder R (2006) Nat Mater 5:118–122
Qian X, Peng XH, Ansari DO et al (2008) Nat Biotechnol 26:83–90
Popovtzer R, Agrawal A, Kotov NA et al (2008) Nano Lett 8:4593–4596
Cai QY, Kim SH, Choi KS, Kim et al (2007) Invest. Radiol 42:797–806
Kim D, Park S, Lee JH et al (2007) J Am Chem Soc 129:7661–7665
Hainfeld JF, Slatkin DN, Focella TMet al (2006) Br J Radiol 79:248–253
Hayat MA (1991) Colloidal gold: principles, methods and applications, volume 1. Academic, San Diego
Slot JW, Geuze HJ (1985) Eur J Cell Biol 38:87–93
Hermanson GT (2008) Bioconjugate techniques, 2nd ed. Elsevier/Academic Press, London
Ambrose J, Hounsfield G (1973) Br J Radiol 46:1016–1047
Su H, Bodenstein C, Dumont R et al (2006) Clin Cancer Res 12:5659–5667
Chung JK, Lee YJ, Kim SK et al (2004) Nucl Med Commun 25:11–17
Barnett JEG, Holman GD, Munday KA (1973) Biochem J 131:211–221
Lampidis TJ, Kurtoglu M et al (2004) Cancer Chemother Pharmacol 53:116–122
Landau BR, Spring-Robinson CL, Muzic RF Jr et al (2007) Am J Physiol Endocrinol Metab 293:E237–E245
Etzioni R, Urban N, Ramsey S et al (2003) Nat Rev Cancer 3:242–252
Acknowledgement
This work was partially supported by Research Training in Medical Physics 5 T32-EB002103-19. Use of the Center for Nanoscale Materials at Argonne National Laboratory was supported by the US Department of Energy, Office of Science, Office of Basic Energy Science, under Contract No. DE-AC02-06CH11357.
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Aydogan, B., Li, J., Rajh, T. et al. AuNP-DG: Deoxyglucose-Labeled Gold Nanoparticles as X-ray Computed Tomography Contrast Agents for Cancer Imaging. Mol Imaging Biol 12, 463–467 (2010). https://doi.org/10.1007/s11307-010-0299-8
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DOI: https://doi.org/10.1007/s11307-010-0299-8