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Combined I-124 Positron Emission Tomography/Computed Tomography Imaging of NIS Gene Expression in Animal Models of Stably Transfected and Intravenously Transfected Tumor

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Abstract

Purpose

With the advent of replication competent viruses for cancer gene therapy, it has become imperative to monitor the biodistribution, expression and replication of these vectors in living organisms. We evaluated the potential of I-124 positron emission tomography (PET)/computed tomography (CT) imaging in gene therapy animal models utilizing the sodium iodide symporter (NIS) and compared the findings to I-123 gamma camera imaging.

Procedures

CB17 SCID mice were implanted with myeloma cell lines expressing NIS or infected by MV-NIS given systemically. Mice were imaged by both gamma camera (I-123) and PET/CT (I-124 ) and image quality assessed.

Results

NIS expressing tumors concentrated 7.1% of the injected activity while tumors infected with the control virus had only 0.3% of the activity injected.

Conclusions

I-124 PET/CT in combination with NIS allows the tracking of stably transfected tumors or intravenously transfected tumors. Combined modality imaging using PET/CT allows accurate and non-invasive imaging of the distribution and gene expression of a replicating viral vector in living systems.

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Acknowledgments

The authors acknowledge the expert technical assistance of Royce Ruter (gamma camera imaging), Mary E. Harvey (veterinary technologist) and Dr Rafael Fonseca, Mayo Clinic, Rochester for the MM1 cell line. This work was supported by grants CA 83181 and CA 91956 from the National Institutes of Health, the Multiple Myeloma Research Foundation (MMRF) and Mayo Foundation.

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Authors and Affiliations

  1. Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN, 55905, USA

    David Dingli & Stephen J. Russell

  2. Division of Hematology, Mayo Clinic College of Medicine, Rochester, MN, 55905, USA

    David Dingli & Stephen J. Russell

  3. Department of Radiology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN, 55905, USA

    Brad J. Kemp, Michael K. O’Connor & Val J. Lowe MD

  4. Division of Endocrinology and Metabolism, Mayo Clinic College of Medicine, Rochester, MN, 55905, USA

    John C. Morris

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  1. David Dingli
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  2. Brad J. Kemp
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  3. Michael K. O’Connor
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  4. John C. Morris
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  5. Stephen J. Russell
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  6. Val J. Lowe MD
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Correspondence to Val J. Lowe MD.

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Dingli, D., Kemp, B.J., O’Connor, M.K. et al. Combined I-124 Positron Emission Tomography/Computed Tomography Imaging of NIS Gene Expression in Animal Models of Stably Transfected and Intravenously Transfected Tumor. Mol Imaging Biol 8, 16–23 (2006). https://doi.org/10.1007/s11307-005-0025-0

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