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1H NMR-based metabolomics approach reveals metabolic alterations in response to dietary imbalances in Megalobrama amblycephala

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Abstract

Introduction

High-fat and high-carbohydrate diets cause a number of metabolic disorders in mammals. However, little is known about metabolomic changes caused by dietary imbalances in fish.

Objectives

The objective of this study was to assess the impacts of high-fat diet (HFD), high-carbohydrate diet (HCD) and high-fat-high-carbohydrate diet (HFHCD) on metabolites in a farmed cyprinid fish Megalobrama amblycephala.

Methods

We have employed the 1H NMR-based metabolomic approach to measure the concentrations of metabolites in plasma and liver of four different diet groups: HFD, HCD, HFHCD and control. Multivariate statistical analyses were used to determine significantly changed metabolites between all group-pairs.

Results

All three test diets have affected metabolic profiles, phenotypes and clinical chemistry. High-fat diets (HFD, HFHCD) resulted in a higher average weight than HCD, but high-carbohydrate diets (HCD, HFHCD) caused signs of liver damage. HCD has resulted in elevated metabolites in energy pathways, leading to further disturbances in creatine pathway. Excess of carbohydrate and lipid metabolism products in the HFHCD group appears to have caused “congestion” of the TCA cycle, causing a significant decline in the numbers of amino acids entering the cycle, which in turn resulted in elevated levels of seven amino acids in this group. Gut microbiota metabolites (TMA) exhibited a strong positive correlation with the carbohydrate content and a negative correlation with the fat content in diets.

Conclusion

These results provide an important insight into the diet-affected metabolic disorders that often lead to financial losses in the aquaculture of Megalobrama amblycephala.

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Acknowledgements

This study was supported by Natural Science Foundation of China (No. 31401976), the earmarked fund for the Fundament Research Funds for the Central Universities (2662015PY019) and Modern Agro-industry Technology Research System entitled ‘‘Staple Freshwater Fishery Industry Technology System’’ (No.CARS-46-05).

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Authors and Affiliations

  1. Key Lab of Freshwater Animal Breeding, Key Lab of Agricultural Animal Genetics, Ministry of Agriculture, Breeding and Reproduction of Ministry of Education, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Panita Prathomya, Wassana Prisingkorn, Fang-Yu Deng, Yu-Hua Zhao & Wei-Min Wang

  2. Bio-Transduction Lab, Wuhan Institute of Biotechnology, Wuhan, 430075, People’s Republic of China

    Ivan Jakovlić

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  1. Panita Prathomya
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  2. Wassana Prisingkorn
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  3. Ivan Jakovlić
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  4. Fang-Yu Deng
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  5. Yu-Hua Zhao
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Correspondence to Yu-Hua Zhao or Wei-Min Wang.

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Prathomya, P., Prisingkorn, W., Jakovlić, I. et al. 1H NMR-based metabolomics approach reveals metabolic alterations in response to dietary imbalances in Megalobrama amblycephala . Metabolomics 13, 17 (2017). https://doi.org/10.1007/s11306-016-1158-7

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