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Metabolite profiling of developing Camelina sativa seeds

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Abstract

Introduction

Camelina sativa is a Brassicaceae with interesting agronomic potential and is considered an alternative oilseed crop. Currently, Camelina is grown mainly for its seed, which shows a high oil content with an unusual fatty acid profile particularly rich in polyunsaturated fatty acids. Camelina seeds contain other potentially valuable compounds and their composition is now relatively well described. However, little information is available on the accumulation dynamics of these compounds during seed development.

Objectives

Our aim is to describe the dynamics of metabolites accumulation during C. sativa seed development.

Methods

After purification by HPLC, the fractions were analyzed by LC–MS and NMR to characterize new compounds. The dynamic of metabolites accumulation during seed development was monitored during 15, 25 and 35 days after flowering, and metabolic profilings were performed by LC–MS and GC–MS.

Results

This study describes for the first time two compounds (quercetin-5b-O-sinapyl-2″-O-apiosyl-3-O-rutinoside and epicatechin-7-O-glucose) that have not previously been identified in the seeds of C. sativa. We also show the accumulation kinetics of various metabolites involved in seed development. These investigations highlight a major reorganization of the metabolome with a depletion of the content of most primary metabolites and a high accumulation of most fatty acids, glucosinolates, flavonoids and sinapic acid derivatives.

Conclusion

This study resulted in the metabolic profile of C. sativa during seed development and enabled to identify two novel compounds in Camelina seeds.

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Acknowledgements

This work was performed, in partnership with the SAS PIVERT, within the framework of the French Institute for Energy Transition (“Institut pour la Transition Energétique”—ITE) P.I.V.E.R.T. (www.institut-pivert.com < http://www.institut-pivert.com>) selected as an Investment for the Future (“Investissements d’Avenir”). This work was supported, as part of Investments for the Future, by the French Government under the reference ANR-001-01. The European Regional Development Fund (equipment acquired) is gratefully acknowledged.

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Authors and Affiliations

  1. EA 3900-BIOPI Biologie des Plantes et Innovation, Département Génie Biologique, Le Bailly et Faculté de Pharmacie, IUT d’Amiens, Université de Picardie Jules Verne, Avenue des Facultés, 1, rue des Louvels, 80025, Amiens Cedex, France

    Anthony Quéro, Roland Molinié, Benjamin Thiombiano, Jean-Xavier Fontaine, Déborah Brancourt, Olivier Van Wuytswinkel, Emmanuel Petit & François Mesnard

  2. Université de Picardie Jules Verne, Plate-Forme Analytique, 33 rue Saint Leu, 80039, Amiens, France

    David Mathiron & Serge Pilard

  3. Centre de Ressources Régionales en Biologie Moléculaire, Université de Picardie Jules Verne, 80039, Amiens, France

    Hervé Demailly & Gaëlle Mongelard

  4. Sorbonne Universités, CNRS-FRE 3580, GEC, Université de Technologie de Compiègne, CS 60319, Compiègne Cedex, 60203, France

    Brigitte Thomasset

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  1. Anthony Quéro
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  2. Roland Molinié
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  12. Brigitte Thomasset
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  13. François Mesnard
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Corresponding author

Correspondence to François Mesnard.

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Electronic supplementary material

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Supplementary material 1

Summary table of LC-MS analysis. Supplementary material 1 (XLSX 15 kb)

Supplementary material 2

NMR analysis for quercetin-5b-O-sinapyl-2′′-O-apiosyl-3-O-rutinoside. Supplementary material 2 (DOCX 1033 kb)

Supplementary material 3

NMR analysis for epicatechin-7-O-glucose. Supplementary material 3 (DOCX 1083 kb)

Supplementary material 4

Supplementary material 4 (XLSX 10 kb)

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Quéro, A., Molinié, R., Mathiron, D. et al. Metabolite profiling of developing Camelina sativa seeds. Metabolomics 12, 186 (2016). https://doi.org/10.1007/s11306-016-1135-1

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