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Physiological and environmental parameters associated with mass spectrometry-based salivary metabolomic profiles

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Abstract

Mass spectrometry (MS)-based metabolomic methods enable simultaneous profiling of hundreds of salivary metabolites, and may be useful to diagnose a wide range of diseases using saliva. However, few studies have evaluated the effects of physiological or environmental factors on salivary metabolomic profiles. Therefore, we used capillary electrophoresis-MS to analyze saliva metabolite profiles in 155 subjects with reasonable oral hygiene, and examined the effects of physiological and environmental factors on the metabolite profiles. Overall, 257 metabolites were identified and quantified. The global profiles and individual metabolites were evaluated by principle component analysis and univariate tests, respectively. Collection method, collection time, sex, body mass index, and smoking affected the global metabolite profiles. However, age also might contribute to the bias in sex and collection time. The profiles were relatively unaffected by other parameters, such as alcohol consumption and smoking, tooth brushing, or the use of medications or nutritional supplements. Temporomandibular joint disorders had relatively greater effects on salivary metabolites than other dental abnormalities (e.g., stomatitis, tooth alignment, and dental caries). These findings provide further insight into the diversity and stability of salivary metabolomic profiles, as well as the generalizability of disease-specific biomarkers.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (B) (KAKENHI 23390420) and by research funds from the Yamagata Prefectural Government and the city of Tsuruoka. We wish to thank all of the volunteers at Yokohama School of Dental Technology and Kanagawa Dental College who provided saliva samples.

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Authors and Affiliations

  1. Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata, 997-0052, Japan

    Masahiro Sugimoto, Hiromi Onuma, Miku Kaneko, Tomoyoshi Soga & Masaru Tomita

  2. Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa, 252-8520, Japan

    Masahiro Sugimoto, Tomoyoshi Soga & Masaru Tomita

  3. Graduate School of Medicine and Faculty of Medicine, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto, 606-8501, Japan

    Masahiro Sugimoto

  4. Department of Pathology, Kanagawa Dental College, Post Graduate School, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan

    Masahiro Sugimoto, Juri Saruta, Chisa Matsuki, Masahiro To & Keiichi Tsukinoki

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  1. Masahiro Sugimoto
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  2. Juri Saruta
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Correspondence to Masahiro Sugimoto.

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Sugimoto, M., Saruta, J., Matsuki, C. et al. Physiological and environmental parameters associated with mass spectrometry-based salivary metabolomic profiles. Metabolomics 9, 454–463 (2013). https://doi.org/10.1007/s11306-012-0464-y

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  • DOI: https://doi.org/10.1007/s11306-012-0464-y

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