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Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum

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Abstract

A recent comparative proteomics study identified the short-chain dehydrogenase (Sch1) as being required for asexual sporulation (Tan et al. Eukaryotic Cell 7:1916–1929, 2008). Metabolite profiling was undertaken on the mutant strains of Stagonospora nodorum lacking the Sch1 gene to help elucidate its role. Gas chromatography-mass spectrometry of the polar metabolites in the Sch1 mutants identified a secondary metabolite at a 200-fold greater concentration than observed in the wild-type strains. Comparative analysis of the secondary metabolite and the mycotoxin alternariol using ESI-MS/MS confirmed the identity of the compound as alternariol. This is the first report to confirm the presence of a mycotoxin in S. nodorum and compelling the field to consider the health implication of this disease.

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Acknowledgements

This research was supported by the Grains Research and Development Corporation. K.-C.T. was funded by an Australian Postgraduate Award.

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Authors and Affiliations

  1. Australian Centre for Necrotrophic Fungal Pathogens, SABC, Faculty of Health Sciences, Murdoch University, Murdoch, WA, 6150, Australia

    Kar-Chun Tan & Richard P. Oliver

  2. Separation Science Laboratory, Faculty of Health Sciences, Murdoch University, Murdoch, WA, 6150, Australia

    Robert D. Trengove

  3. Metabolomics Australia, Murdoch University, Perth, WA, 6150, Australia

    Robert D. Trengove, Richard P. Oliver & Peter S. Solomon

  4. School of Pharmacy, Faculty of Health Sciences, Murdoch University, Murdoch, WA, 6150, Australia

    Garth L. Maker

  5. Plant Cell Biology, School of Biology (RSBS), The Australian National University, Canberra, ACT 0200, Australia

    Peter S. Solomon

Authors
  1. Kar-Chun Tan
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  2. Robert D. Trengove
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  3. Garth L. Maker
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  4. Richard P. Oliver
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  5. Peter S. Solomon
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Correspondence to Peter S. Solomon.

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Tan, KC., Trengove, R.D., Maker, G.L. et al. Metabolite profiling identifies the mycotoxin alternariol in the pathogen Stagonospora nodorum . Metabolomics 5, 330–335 (2009). https://doi.org/10.1007/s11306-009-0158-2

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  • DOI: https://doi.org/10.1007/s11306-009-0158-2

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