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Quantification of 1H NMR spectra of human cerebrospinal fluid: a protocol based on constrained total-line-shape analysis

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Abstract

A protocol for quantitative 1H NMR analysis of human cerebrospinal fluid (hCSF) was built up and assessed as based on Constrained Total-Line-Shape (CTLS) fitting. In this method, linear constraints were applied to spectral structures. The 1H NMR spectra of 45 human CSF samples were measured and quantified using the CTLS method. The quantification strategies based on total-line-shape fitting are discussed. The metabolic model for CTLS includes 31 metabolites covering 85% of the total spectral intensity, excluding the protein contribution. Prior to data analysis, the data was divided into patients with no Alzheimer’s disease (AD), but with a normal AD marker profile (the peptide β-amyloid42 and tau protein) present in CSF, and into controls that do not have an AD marker profile in CSF. Unexpectedly large variations in metabolite concentrations within the two patient groups were detected, but an analysis of variance revealed a significant (P = 0.027) difference only in the concentration of creatinine which was higher in patients that had a normal AD marker profile. Multivariate classification tools such as self-organizing maps (SOM) failed in separation of the two classes.

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Acknowledgements

This work was supported by the National Technology Agency of Finland (TEKES), EVO grant of Kuopio University Hospital (5772720), Sigrid Jusélius Foundation, Maire Taponen Foundation and the Culture Fund of Finland.

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Authors and Affiliations

  1. Laboratory of Chemistry, Department of Biosciences, University of Kuopio, P.O. Box 1627, 70211, Kuopio, Finland

    Niko M. Jukarainen, Samuli-Petrus Korhonen, Pasi P. Soininen, Kari Tuppurainen, Jouko Vepsäläinen & Reino Laatikainen

  2. Department of Neurology, Kuopio University Hospital, Kuopio, Finland

    Mikko P. Laakso & Tuula Pirttilä

  3. Department of Clinical Radiology, Kuopio University Hospital, Kuopio, Finland

    Mikko P. Laakso

  4. Department of Neuroscience and Neurology, University of Kuopio, Kuopio, Finland

    Minna A. Korolainen

  5. Brain Research Unit, Clinical Research Centre/Mediteknia, University of Kuopio, Kuopio, Finland

    Minna A. Korolainen & Tuula Pirttilä

  6. PERCH Solutions Ltd, Kuopio, Finland

    Matthias Niemitz

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  1. Niko M. Jukarainen
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  2. Samuli-Petrus Korhonen
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  3. Mikko P. Laakso
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  4. Minna A. Korolainen
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  6. Pasi P. Soininen
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  7. Kari Tuppurainen
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  8. Jouko Vepsäläinen
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  10. Reino Laatikainen
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Correspondence to Niko M. Jukarainen.

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Jukarainen, N.M., Korhonen, SP., Laakso, M.P. et al. Quantification of 1H NMR spectra of human cerebrospinal fluid: a protocol based on constrained total-line-shape analysis. Metabolomics 4, 150–160 (2008). https://doi.org/10.1007/s11306-008-0106-6

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  • DOI: https://doi.org/10.1007/s11306-008-0106-6

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