A strategy for robust and reliable mechanistic statistical modelling of metabolic responses in relation to drug induced toxicity is presented. The suggested approach addresses two cases commonly occurring within metabonomic toxicology studies, namely; 1) A pre-defined hypothesis about the biological mechanism exists and 2) No such hypothesis exists. GC/MS data from a liver toxicity study consisting of rat urine from control rats and rats exposed to a proprietary AstraZeneca compound were resolved by means of hierarchical multivariate curve resolution (H-MCR) generating 287 resolved chromatographic profiles with corresponding mass spectra. Filtering according to significance in relation to drug exposure rendered in 210 compound profiles, which were subjected to further statistical analysis following correction to account for the control variation over time. These dose related metabolite traces were then used as new observations in the subsequent analyses. For case 1, a multivariate approach, named Target Batch Analysis, based on OPLS regression was applied to correlate all metabolite traces to one or more key metabolites involved in the pre-defined hypothesis. For case 2, principal component analysis (PCA) was combined with hierarchical cluster analysis (HCA) to create a robust and interpretable framework for unbiased mechanistic screening. Both the Target Batch Analysis and the unbiased approach were cross-verified using the other method to ensure that the results did match in terms of detected metabolite traces. This was also the case, implying that this is a working concept for clustering of metabolites in relation to their toxicity induced dynamic profiles regardless if there is a pre-existing hypothesis or not. For each of the methods the detected metabolites were subjected to identification by means of data base comparison as well as verification in the raw data. The proposed strategy should be seen as a general approach for facilitating mechanistic modelling and interpretations in metabolomic studies.
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Acknowledgments
This work was supported by grants from the EU-strategic funding, Strategic Research Foundation (SSF), the Swedish Association of Persons with Neurologically Disabilities (NHR), the Swedish Research Council, Wallenberg Consortium North (WCN), and the Kempe Foundation. Elwin R. Verheij, Leo van Stee and Bas Muiljwijk, TNO Pharma are gratefully acknowledged for provision of the GC/MS. Hans Stenlund, Umeå University, is gratefully acknowledged for provision of the Matlab script for raw data interpretation.
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Pohjanen, E., Thysell, E., Lindberg, J. et al. Statistical multivariate metabolite profiling for aiding biomarker pattern detection and mechanistic interpretations in GC/MS based metabolomics. Metabolomics 2, 257–268 (2006). https://doi.org/10.1007/s11306-006-0032-4
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DOI: https://doi.org/10.1007/s11306-006-0032-4