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Transcriptome sequencing analysis of two different genotypes of Asian pear reveals potential drought stress genes

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Abstract

‘Huangguan’ (HG) and ‘Whangkeumbae’ (HK) pears are two important Asian pear cultivars in China and South Korea, respectively. In practical production, ‘Huangguan’ pear is a drought-tolerant genotype while ‘Whangkeumbae’ pear is drought-sensitive. To uncover the mechanisms underlying pear tree tolerance to drought stress, a comprehensive transcriptome analysis was performed in this study. The results revealed a total of 1185 and 1667 differently expressed genes (DEGs) between control and treated plants of HG and HK pear, respectively. KEGG pathways enrichment analysis revealed that the DEGs were involved in the metabolism and signal transduction of various phytohormones. In HG, the DEGs annotated as ABA, gibberellic acid (GA), and salicylic acid (SA) were all upregulated, while those DEGs annotated as jasmonic acid (JA) were upregulated or downregulated. In HK, the DEGs annotated as ABA and JA were both upregulated or downregulated, but there were no DEGs annotated as GA and SA. In addition, there were 743 DEGs expressed in HG, but not in HK. Among them, there were 288 DEGs whose absolute values of log2(fold-change ratio) were greater than 2. Eight of the 288 DEGs were selected randomly for validating the reproducibility and accuracy of the transcriptome RNA-Seq data by using quantitative-PCR. Our results will be helpful for breeding drought-tolerant pear cultivars.

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Funding

This project was financially supported by the earmarked fund for the China Agriculture Research System (CARS-29-14) and the Natural Science Research Fund of Higher Education Institutions, Anhui Province (KJ2016A237).

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Author notes

  1. Hua Wang and Ziyu Wang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Pomology, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    Hua Wang, Ziyu Wang, Ming Zhang, Bing Jia, Wei Heng, Zhenfeng Ye & Liwu Zhu

  2. School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    Xiaoniu Xu

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  1. Hua Wang
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  2. Ziyu Wang
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Contributions

HW and ZYW designed and performed the experiment, analyzed the data, drew figures, and wrote the manuscript; MZ, BJ, WH, and ZFY helped in implementing the experiments. LWZ and XNX were supervisors for experiment planning and revised the manuscript. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Liwu Zhu or Xiaoniu Xu.

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Conflict of interest

The authors declare that they have no competing interests.

Data archiving statement

The sequence data has been deposited in the National Center for Biotechnology Information (NCBI) Short Read Archive (SPA) under accession number SRP125844.

Additional information

Communicated by D. Chagné

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Wang, H., Wang, Z., Zhang, M. et al. Transcriptome sequencing analysis of two different genotypes of Asian pear reveals potential drought stress genes. Tree Genetics & Genomes 14, 40 (2018). https://doi.org/10.1007/s11295-018-1249-x

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