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Functional analysis of SOC1-like and AGL6-like MADS-box genes of the gymnosperm Cryptomeria japonica

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Abstract

We are interested in controlling strobili production in Cryptomeria japonica D. Don by genetic engineering. However, details of the molecular mechanisms governing reproduction in gymnosperms have not been documented. In this study, we characterized the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1)-like (CjMADS15) and AGAMOUS-LIKE 6 (AGL6)-like (CjMADS14) MADS-box genes of C. japonica. Their expression patterns differed in that CjMADS15 was expressed in all organs except pollen, and was especially expressed in needles, stems and strobili, whereas CjMADS14 was expressed mainly in male and female strobili. Furthermore, gibberellic acid (GA3)-induced expression of CjMADS14 and CjMADS15 also differed; expression of CjMADS15 increased within a week of GA3 treatment, whereas expression of CjMADS14 increased within 1 month. Both genes had the potential to induce early reproduction, because transgenic Arabidopsis that ectopically expressed CjMADS14 or CjMADS15 flowered earlier than wild-type plants. When we overexpressed a fusion of CjMADS14 to a transcriptional repression domain (SRDX) from SUPERMAN under control of the cauliflower mosaic virus (CaMV) 35S promoter (35S), the flowering time of transgenic Arabidopsis with the 35S::CjMADS14:SRDX construct was comparable with that of 35S::CjMADS14. Thus, CjMADS14 and CjMADS15 play important roles during the development of male and female strobili in C. japonica. Our finding will contribute to elucidate the molecular mechanisms for the formation of reproductive organs in gymnosperms.

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Acknowledgments

We thank Ms. N. Akashi for her technical assistance. This research was supported in part by a Grant-in-Aid (Development of Technologies for Control of Pollen Production by Genetic Engineering) from the Forest Agency of Japan.

Data Archiving Statement

CjMADS14 (accession number AB359029) and CjMADS15 (AB359030) cDNA sequences were submitted to GenBank. Furthermore, two DEF/GLO-like (CjMADS6: AB359036 and CjMADS9: AB359035), AG-like (CjMADS4: AB359031) and TM8-like (CjMADS12: AB359028) cDNA sequence were also submitted to GenBank.

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  1. Shin-Ichiro Katahata

    Present address: Graduate School of Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan

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  1. Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, Ibaraki, 305-8687, Japan

    Shin-Ichiro Katahata, Norihiro Futamura, Tomohiro Igasaki & Kenji Shinohara

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  1. Shin-Ichiro Katahata
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Correspondence to Norihiro Futamura.

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Katahata, SI., Futamura, N., Igasaki, T. et al. Functional analysis of SOC1-like and AGL6-like MADS-box genes of the gymnosperm Cryptomeria japonica . Tree Genetics & Genomes 10, 317–327 (2014). https://doi.org/10.1007/s11295-013-0686-9

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