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A rubber tree’s durable resistance to Microcyclus ulei is conferred by a qualitative gene and a major quantitative resistance factor

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Abstract

The components of genetic resistance from the Hevea brasiliensis cultivar MDF 180 against South American Leaf Blight (SALB) caused by Microcyclus ulei were investigated by QTL mapping. MDF 180 has already been described as a cultivar with a high level of partial and long-lasting resistance. The resistance of progeny individuals from a cross between a susceptible cultivar and MDF 180 was assessed both under controlled conditions of inoculation by three M. ulei isolates and under natural infection in a field trial. Genetic maps of the two parents of this progeny were mainly established based on microsatellites and AFLP markers. No resistance QTL were found in the susceptible parent. In the resistant parent, we identified a qualitative gene responsible for the resistance against isolates from French Guiana and a major quantitative resistance factor determining the resistance against isolates from the state of Bahia (Brazil). The qualitative resistance gene was denominated M15md and was located in the linkage group g15. Four minor resistance QTLs were also identified, two of which showed an epistatic interaction with M15md. The durability of the resistance of MDF 180 is discussed in light of these data.

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Acknowledgments

This work was performed as a part of the Cirad–Michelin collaborative project “Genesalb,” supported by a grant from the French national research agency (Agence National de la Recherche; contract ANR/Génoplante n° GPLA07017C). We thank the Montpellier Languedoc-Roussillon Grand Plateau Technique Régional for hosting the SSR genotyping activities.

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Authors and Affiliations

  1. CIRAD, UMR DAP, 34398, Montpellier, France

    Vincent Le Guen, Dominique Garcia, Sandra Espéout & Marc Seguin

  2. CIRAD, UPR Bioagresseurs des Pérennes, BP 701, 97387, Kourou, French Guiana

    Fabien Doaré, Virgile Condina & Christophe Couturier

  3. Plantações Michelin da Bahia, Cx Postal 2, CEP 45435-000, Ituberá, BA, Brazil

    Carlos R. R. Mattos

  4. INRA, UMR 1318, Route de St-Cyr, 78026, Versailles, France

    Aurélie Chambon

  5. BIOMERIEUX, Centre Christophe Mérieux, 5 rue des Berges, 38000, Grenoble, France

    Christelle Weber

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  1. Vincent Le Guen
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  2. Dominique Garcia
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  3. Fabien Doaré
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  4. Carlos R. R. Mattos
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  9. Sandra Espéout
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  10. Marc Seguin
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Correspondence to Vincent Le Guen.

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Communicated by D. Grattapaglia

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Le Guen, V., Garcia, D., Doaré, F. et al. A rubber tree’s durable resistance to Microcyclus ulei is conferred by a qualitative gene and a major quantitative resistance factor. Tree Genetics & Genomes 7, 877–889 (2011). https://doi.org/10.1007/s11295-011-0381-7

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  • DOI: https://doi.org/10.1007/s11295-011-0381-7

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