Skip to main content
SpringerLink
Log in

Spatial variation in local pollen flow and mating success in a Picea abies clone archive and their implications for a novel “breeding without breeding” strategy

  • Original Paper
  • Published:
Tree Genetics & Genomes Aims and scope Submit manuscript

Abstract

Currently, Norway spruce (Picea abies) breeding in Sweden is based on crosses between the best clones followed by clonal testing of the progenies to select for the long-term breeding population. An alternative breeding strategy called “Breeding without Breeding” (BwB) is proposed, which, in principle, relies on the DNA marker-based pedigree reconstruction from wind-pollinated progenies instead of controlled crosses. To test whether the pedigree structure could be established from progenies of clonal trials, we investigated the spatial pattern of local pollen flow and paternity assignment in a clone archive of Norway spruce. The results showed that 42% of the progeny can be assigned to fathers within 30-m distance with high confidence. Effective pollen dispersal decreased rapidly with distance and followed exponential distribution on local scale. The extent of close-neighbor (within 6 m) mating ranged from 0% to 48% among grafts with an average of 13%. Distance explained 25% deviance in mating success, and other factors such as phenology and spatial configuration of the clones should have contributed the rest. The success of parentage assignment in clone archive opens up the possibility to apply BwB in clonal trials of species that are easy to propagate vegetatively. This procedure could substantially shorten the breeding cycle and still give similar gain per year as the conventional breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Besnard G, Achere V, Rampant PF, Favre JM, Jeandroz S (2003) A set of cross-species amplifying microsatellite markers developed from DNA sequence databanks in Picea (Pinaceae). Mol Ecol Notes 3:380–383

    Article  CAS  Google Scholar 

  • Brenner CH (1997) Symbolic kinship program. Genetics 145:535–542

    PubMed  CAS  Google Scholar 

  • Burczyk JL, Prat D (1997) Male reproductive success in Pseudotsuga menziesii (Mirb.) Franco: the effects of spatial structure and flowering characteristics. Heredity 79:638–647

    Article  Google Scholar 

  • Burczyk J, Adams WT, Shimizu JY (1996) Mating patterns and pollen dispersal in a natural Knobcone pine (Pinus attenuata Lemmon) stand. Heredity 77:251–260

    Google Scholar 

  • Burczyk J, Lewandowski A, Chałupka W (2004) Local pollen dispersal and distant gene flow in Norway spruce (Picea abies [L.] Karst.). For Ecol Manage 197:39–48

    Article  Google Scholar 

  • Danusevicius D, Lindgren D (2002) Efficiency of selection based on phenotype, clone and progeny testing in long-term breeding. Silvae Genet 51:19–26

    Google Scholar 

  • El-Kassaby YA, Lstiburek M (2009) Breeding without breeding. Genet Res 91:111–120

    Article  Google Scholar 

  • El-Kassaby YA, Lstibůrek M, Liewlaksaneeyanawin C, Slavov GT, Howe GT (2007) Breeding without breeding: approach, example, and proof of concept. In: Isik F (ed) Proceedings of the IUFRO division 2 joint conference: low input breeding and conservation of forest genetic resources. Antalya, Turkey, pp 43–54

    Google Scholar 

  • Erickson VJ, Adams WT (1989) Mating success in a coastal Douglas-fir seed orchards as affected by distance and floral phenology. Can J For Res 19:1248–1255

    Article  Google Scholar 

  • Evett IW, Weir BS (1998) Interpreting DNA evidence: statistical genetics for forensic scientists. Sinauer Associates, Sunderland

    Google Scholar 

  • Goto S, Miyahara F, Ide Y (2002) Monitoring male reproductive success in a Japanese black pine clonal seed orchard with RAPD markers. Can J For Res 32:983–988

    Article  CAS  Google Scholar 

  • Goudet J (1995) FSTAT (Version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Hardy OJ, Vekemans X (2002) SPAGEDi: a versatile computer program to analyse spatial genetic structure at the individual or population levels. Mol Ecol Notes 2:618–620

    Article  Google Scholar 

  • Hardy OJ, Gonzalez-Martinez SC, Colas B, Freville H, Mignot A, Olivieri I (2004) Fine-scale genetic structure and gene dispersal in Centaurea corymbosa (Asteraceae). II. Correlated paternity within and among sibships. Genetics 168:1601–1614

    Article  PubMed  Google Scholar 

  • Hodgetts RB, Aleksiuk MA, Brown A, Clarke C, Macdonald E, Nadeem S et al (2001) Development of microsatellite markers for white spruce (Picea glauca) and related species. Theor Appl Genet 102:1252–1258

    Article  CAS  Google Scholar 

  • Hoffmann JP (2003) Generalized linear models: an applied approach. Allyn & Bacon, Boston

    Google Scholar 

  • Kalinowski ST, Taper ML, Marshall TC (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099–1106

    Article  PubMed  Google Scholar 

  • Kang KS, Bila AD, Harju AM, Lindgren D (2003) Estimation of fertility variation in forest tree populations. Forestry 76:330–344

    Article  Google Scholar 

  • Karlsson B, Rosvall O (1993) Breeding programs in Sweden—Norway spruce: proceedings of the Nordic group for tree breeding: progeny testing and breeding strategies. Edinburgh, Scotland, pp 128–134

    Google Scholar 

  • Kimoto S (1967) Some quantitative analysis on the chrysomelid fauna of Ryukyu Archipelago. Esakia 6:27–54

    Google Scholar 

  • Kjaer ED (1996) Estimation of effective population number in a Picea abies (Karst) seed orchard based on flower assessment. Scand J Forest Res 11:111–121

    Article  Google Scholar 

  • Lindgren D (2009) Picea abies breeding in Sweden is based on clone testing. Dendrobiology 61 Supplement: 79–82

    Google Scholar 

  • Lindgren D, Nilsson J-E (1985) Calculations concerning selection intensity. Dept. of Forest Genetics and Plant Physiology. The Swedish University of Agricultural Science, Umeå

    Google Scholar 

  • Loiselle BA, Sork VL, Nason J, Graham C (1995) Spatial genetic structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae). Am J Bot 82:1420–1425

    Article  Google Scholar 

  • Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220

    PubMed  CAS  Google Scholar 

  • Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655

    Article  PubMed  CAS  Google Scholar 

  • Moriguchi Y, Tsuchiya S, Iwata H, Itoo S, Tani N, Taira H et al (2007) Factors influencing male reproductive success in a Cryptomeria japonica seed orchard revealed by microsatellite marker analysis. Silvae Genet 56:207–214

    Google Scholar 

  • Nikkanen T, Ruotsalainen S (2000) Variation in flowering abundance and its impact on the genetic diversity of the seed crop in a Norway spruce seed orchard. Silva Fenn 34:205–222

    Google Scholar 

  • Pakkad G, Ueno S, Yoshimaru H (2008) Gene flow pattern and mating system in a small population of Quercus semiserrata Roxb. (Fagaceae). For Ecol Manage 255:3819–3826

    Article  Google Scholar 

  • Pakkanen A, Nikkanen T, Pulkkinen P (2000) Annual variation in pollen contamination and outcrossing in a Picea abies seed orchard. Scand J Forest Res 15:399–404

    Article  Google Scholar 

  • Pena SDJ, Chakraborty R (1994) Paternity testing in the DNA era. Trends Genet 10:204–209

    Article  PubMed  CAS  Google Scholar 

  • Pfeiffer A, Olivieri AM, Morgante M (1997) Identification and characterization of microsatellites in Norway spruce (Picea abies K). Genome 40:411–419

    Article  PubMed  CAS  Google Scholar 

  • Pluess AR, Sork VL, Dolan B, Davis FW, Grivet D, Merg K et al (2009) Short distance pollen movement in a wind-pollinated tree, Quercus lobata (Fagaceae). For Ecol Manage 258:735–744

    Article  Google Scholar 

  • Robledo-Arnuncio JJ, Alia R, Gil L (2004) Increased selfing and correlated paternity in a small population of a predominantly outcrossing conifer, Pinus sylvestris. Mol Ecol 13:2567–2577

    Article  PubMed  CAS  Google Scholar 

  • Robledo-Arnuncio JJ, Austerlitz F, Smouse PE (2007) POLDISP: a software package for indirect estimation of contemporary pollen dispersal. Mol Ecol Notes 7:763–766

    Article  Google Scholar 

  • Scotti I, Paglia GP, Magni F, Morgante M (2002) Efficient development of dinucleotide microsatellite markers in Norway spruce (Picea abies Karst.) through dot-blot selection. Theor Appl Genet 104:1035–1041

    Article  PubMed  CAS  Google Scholar 

  • Shen HH, Rudin D, Lindgren D (1981) Study of the pollination pattern in a Scots pine seed orchard by means of isozyme analysis. Silvae Genet 30:7–15

    Google Scholar 

  • Stoehr MU, Newton CH (2002) Evaluation of mating dynamics in a lodgepole pine seed orchard using chloroplast DNA markers. Can J For Res 32:469–476

    Article  CAS  Google Scholar 

  • Streiff R, Ducousso A, Lexer C, Steinkellner H, Gloessl J, Kremer A (1999) Pollen dispersal inferred from paternity analysis in a mixed oak stand of Quercus robur L. and Q. petraea (Matt.) Liebl. Mol Ecol 8:831–841

    Article  Google Scholar 

  • Tomita M, Saito H, Suyama Y (2008) Effect of local stand density on reproductive processes of the sub-boreal conifer Picea jezoensis Carr. (Pinaceae). For Ecol Manage 256:1350–1355

    Article  Google Scholar 

  • Torimaru T, Wang XR, Fries A, Andersson B, Lindgren D (2009) Evaluation of pollen contamination in an advanced Scots pine seed orchard. Silvae Genet 58:262–269

    Google Scholar 

  • Wang XR, Torimaru T, Lindgren D, Fries A (2010) Marker-based parentage analysis facilitates low input 'breeding without breeding' strategies for forest trees. Tree Genet Genomes 6:227–235

    Article  Google Scholar 

Download references

Acknowledgments

We thank Anders Fries for participation in sample collection, and Jenny Arnerup for recommendation of SSR primers. This study was supported by grants from the Swedish Association of Forest Tree Breeding, the Bo Rydins Stiftelse, and Vetenskapsrådet, Sweden.

Author information

Authors and Affiliations

  1. Department of Ecology and Environmental Science, UPSC, Umeå University, SE-901 87, Umeå, Sweden

    Ayako Shimono & Xiao-Ru Wang

  2. Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan

    Takeshi Torimaru

  3. Department of Forest Genetics and Plant Physiology, UPSC, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden

    Dag Lindgren

  4. The Forestry Research Institute of Sweden, Ekebo, SE-268 90, Svalöv, Sweden

    Bo Karlsson

Authors
  1. Ayako Shimono
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiao-Ru Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takeshi Torimaru
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dag Lindgren
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bo Karlsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xiao-Ru Wang.

Additional information

Communicated by Y. Tsumura

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shimono, A., Wang, XR., Torimaru, T. et al. Spatial variation in local pollen flow and mating success in a Picea abies clone archive and their implications for a novel “breeding without breeding” strategy. Tree Genetics & Genomes 7, 499–509 (2011). https://doi.org/10.1007/s11295-010-0351-5

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11295-010-0351-5

Keywords

Search

Navigation