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Gibberellic acid insensitive mRNA transport in both directions between stock and scion in Malus

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Abstract

The sieve tube in higher plants functions as infrastructure for long-distance transport of nutrients, photoassimilates, and growth regulators including hormones. Recently, it was revealed that some protein and RNA molecules also function as movable growth regulators in the sieve tube. In the case of the mRNA of gibberellic acid insensitive (GAI), the transport evidence was obtained through identification of the overproduced transgene transcript. In this work, we investigated the transport of apple (Malus x domestica cv. Fuji and Malus xiaojinensis) endogenous GAI mRNA by grafting experiments. Each GAI mRNA of scion and stock plants was detected in the graft partners as from 5 days after grafting, indicating that the GAI mRNA moves in both upward and downward directions via the graft union.

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Acknowledgements

We thank S. Ozeki and S. Kida (Hirosaki University, Japan) for technical assistance. This work was supported by the Program for Promotion of Basic Activities for Innovative Bioscience (PROBRAIN) in Japan.

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Authors and Affiliations

  1. Laboratory of Fruit cell and Molecular Breeding, China Agriculture University, Beijing, 100193, People’s Republic of China

    Haiyan Xu, Wenna Zhang, Maofu Li & Tianzhong Li

  2. Institute of Horticultural Plants, China Agriculture University, Beijing, 100193, People’s Republic of China

    Zhenhai Han

  3. Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, 036-8561, Japan

    Haiyan Xu & Takeo Harada

Authors
  1. Haiyan Xu
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  2. Wenna Zhang
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  3. Maofu Li
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  4. Takeo Harada
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  5. Zhenhai Han
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  6. Tianzhong Li
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Correspondence to Takeo Harada or Tianzhong Li.

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Communicated by E. Dirlewanger

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Xu, H., Zhang, W., Li, M. et al. Gibberellic acid insensitive mRNA transport in both directions between stock and scion in Malus. Tree Genetics & Genomes 6, 1013–1019 (2010). https://doi.org/10.1007/s11295-010-0309-7

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  • DOI: https://doi.org/10.1007/s11295-010-0309-7

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