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Quantitative proteomic analysis of short photoperiod and low-temperature responses in bark tissues of peach (Prunus persica L. Batsch)

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Abstract

In the temperate climate of the northern hemisphere, winter survival of woody plants is determined by the ability to acclimate to freezing temperatures and to undergo a period of dormancy. Cold acclimation in many woody plants is initially induced by short photoperiod and low, non-freezing temperatures. These two factors (5°C and short photoperiod) were used to study changes in the proteome of bark tissues of 1-year-old peach trees. Difference in-gel electrophoresis technology, a gel-based approach involving the labeling of proteins with different fluorescent dyes, was used to conduct a quantitative assessment of changes in the peach bark proteome during cold acclimation. Using this approach, we were able to identify differentially expressed proteins and to assign them to a class of either ‘temperature-responsive’ or ‘photoperiod-responsive’ proteins. The most significant factor affecting the proteome appeared to be low temperature, while the combination of low temperature and short photoperiod was shown to act either synergistically or additively on the expression of some proteins. Fifty-seven protein spots on gels were identified by mass spectrometry. They included proteins involved in carbohydrate metabolism (e.g., enolase, malate dehydrogenase, etc), defense or protective mechanisms (e.g., dehydrin, HSPs, and PR-proteins), energy production and electron transport (e.g., adenosine triphosphate synthases and lyases), and cytoskeleton organization (e.g., tubulins and actins). The information derived from the analysis of the proteome is discussed as a function of the two treatment factors: low temperature and short photoperiod.

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Abbreviations

2D (E):

bidimensional (electrophoresis)

ABA:

abscisic acid

CHAPS:

3-([3-cholamidopropyl]dimethylammonio)-1-propanesulfonate

DiGE:

difference in-gel electrophoresis

DTT:

dithiothreitol

HSP:

heat-shock protein

IPG:

immobilized pH gradient

LD:

long day

LT:

low temperature

PR-proteins:

pathogenesis-related proteins

SD:

short day

TCA:

trichloroacetic acid

SSH:

suppression subtractive hybridization

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Authors and Affiliations

  1. Department of Environment and Agrobiotechnologies, Centre de Recherche Public–Gabriel Lippmann, 41 rue du Brill, 4422, Belvaux, GD Luxembourg

    Jenny Renaut, Jean-François Hausman & Henry-Michel Cauchie

  2. Appalachian Fruit Research Station, Agricultural Research Service, US Department of Agriculture, 2217 Wiltshire Road, Kearneysville, WV, 25430, USA

    Carole Bassett, Timothy Artlip & Michael Wisniewski

  3. Center for Proteome Analysis and Mass Spectrometry, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium

    Erwin Witters

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  1. Jenny Renaut
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  2. Jean-François Hausman
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  3. Carole Bassett
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  4. Timothy Artlip
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Correspondence to Jenny Renaut.

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Communicated by R. Sederoff

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Summary of the identified proteins, differentially regulated by LT and/or SD or by a combination of both factors, as determined by the ANCOVA. aAccession numbers are issued from ExPASY website (SWISS_Prot database, http://www.expasy.org), bscore = Mascot-MOWSE score, cnumber of peptides matched in databases, dtotal ion score = score calculated by weighting ion scores for all individual peptides matched to a given protein. e P value of the ANCOVA two-ways analysis: in green cells, the abundance values showing an increase; in orange cells, the abundance values showing a decrease. (DOC 292 KB)

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Renaut, J., Hausman, JF., Bassett, C. et al. Quantitative proteomic analysis of short photoperiod and low-temperature responses in bark tissues of peach (Prunus persica L. Batsch). Tree Genetics & Genomes 4, 589–600 (2008). https://doi.org/10.1007/s11295-008-0134-4

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