Abstract
Regulators belonging to the DeoR family are widely distributed among the bacteria. Few studies have reported that DeoR family proteins regulate secondary metabolism of Streptomyces. This study explored the function of DeoR (SLINC_8027) in Streptomyces lincolnensis. Deletion of deoR in NRRL 2936 led to an increase in cell growth. The lincomycin production of the deoR deleted strain ΔdeoR was 3.4-fold higher than that of the wild strain. This trait can be recovered to a certain extent in the deoR complemented strain ΔdeoR::pdeoR. According to qRT-PCR analysis, DeoR inhibited the transcription of all detectable genes in the lincomycin biosynthesis cluster and repressed the expression of glnR, bldD, and SLCG_Lrp, which encode regulators outside the cluster. DeoR also inhibited the transcription of itself, as revealed by the XylE reporter. Furthermore, we demonstrated that DeoR bound directly to the promoter region of deoR, lmbA, lmbC-D, lmbJ-K, lmrA, lmrC, glnR, and SLCG_Lrp, by recognizing the 5ʼ-CGATCR-3’ motif. This study found that versatile regulatory factor DeoR negatively regulates lincomycin biosynthesis and cellular growth in S. lincolnensis, which expanded the regulatory network of lincomycin biosynthesis.
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Data Availability
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by Ministry of Science and Technology (MOST) of China (2021YFC2100600) and the National Natural Science Foundation of China (NSFC) (31900059).
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Jingyun Zou: Methodology, Investigation, Writing - Original Draft. Yue Mao: Investigation, Writing - Review & Editing. Bingbing Hou: Methodology, Formal analysis. Yajing Kang: Investigation, Formal analysis. Ruida Wang: Methodology, Writing - Review & Editing. Haizhen Wu: Methodology, Formal analysis. Jiang Ye: Conceptualization, Methodology. Huizhan Zhang: Supervision, Conceptualization. All authors reviewed the manuscript.
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Zou, J., Mao, Y., Hou, B. et al. DeoR regulates lincomycin production in Streptomyces lincolnensis. World J Microbiol Biotechnol 39, 332 (2023). https://doi.org/10.1007/s11274-023-03788-w
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DOI: https://doi.org/10.1007/s11274-023-03788-w