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Multifarious effect of ACC deaminase and EPS producing Pseudomonas sp. and Serratia marcescens to augment drought stress tolerance and nutrient status of wheat

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Abstract

Drought is the prime abiotic stress that rigorously influences plant growth, yield and quality of crops. The current investigation illustrated the bio-protective characters of Serratia marcescens and Pseudomonas sp. to ameliorate drought stress tolerance, plant growth and nutrient status of wheat. The present study aimed for search of potential drought tolerant plant growth-promoting rhizobacteria (PGPR). All screened bacterial isolates exhibited potential plant growth promoting (PGP) attributes such as production of ACC deaminase, exo-polysaccharide, siderophore, ammonia, IAA, and efficiently solubilized zinc and phosphate under in vitro conditions. To assess the in situ plant growth promotion potential of PGPR, a greenhouse experiment was conducted by priming wheat seeds with screened plant PGPR. Improved water status, reactive oxygen species, osmolyte accumulation, chlorophyll and carotenoids content in plant leaves confirmed the excellent drought tolerance conferring ability of RRN II 2 and RRC I 5. Among all PGPR, RRN II 2 and RRC I 5 inoculated plants not only demonstrated greater harvest index but also exhibited more micronutrient (zinc and iron) content in wheat grains. Further, RRN II 2 and RRC I 5 were identified through 16S rDNA sequencing as S. marcescens and Pseudomonas sp., respectively. Furthermore, amplification of acdS gene (Amplified band size of acdS gene was ~ 1.8 Kb) also confirmed ACC deaminase enzyme producing ability of Pseudomonas sp. Moreover, correlation coefficient, principal component analysis and cluster analysis also demonstrated that nutrient status and values of agronomical parameters of wheat primed with S. marcescens and Pseudomonas sp. were at par with the positive control. Thus, the outcome of this comparative investigation indicates that Pseudomonas sp. and S. marcescens could be utilized as bioinoculant in wheat since they can improve the physiological status, productivity and nutrient status in wheat crop under drought.

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  1. Department of Microbiology, College of Basic Sciences and Humanities, Govind Ballabh Pant University of Agriculture and Technology, U.S. Nagar, Pantnagar, Uttarakhand, 263145, India

    Amir Khan & Ajay Veer Singh

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AK performed research, analyze data and written manuscript; editing of the manuscript and research carried out under the guidance of AVS.

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Khan, A., Singh, A.V. Multifarious effect of ACC deaminase and EPS producing Pseudomonas sp. and Serratia marcescens to augment drought stress tolerance and nutrient status of wheat. World J Microbiol Biotechnol 37, 198 (2021). https://doi.org/10.1007/s11274-021-03166-4

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