Abstract
In this paper, we report the antimicrobial activity of AMEP412 (a protein elicitor from Bacillus subtilis) against Streptomyces scabiei, which is the potato common scab pathogen. The purified protein samples showed an obvious inhibition zone on an S. scabiei agar plate, and the minimum inhibition concentration detected was 50 μg mL−1. The fluorescence localization assay revealed that AMEP412 could bind to aerial mycelia and spores. The stability test showed that AMEP412 was stable at 60 °C for 30 min and in pH values from 5.0 to 10.0. Its antimicrobial activity was not sensitive to metal cations. However, its activity declined by 23% when treated with Proteinase K, and was completely abrogated with Tween 80 treatment. Three antimicrobial peptides (GS21, GY20 and GY23) were identified from AMEP412, which further verified its antimicrobial activity. This research reveals the antimicrobial function of AMEP412, which not only enriches the function of the protein elicitor, but also provides a candidate for the biocontrol of potato common scab.
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Acknowledgements
This study was financially supported by the Natural Science Foundation of Heilongjiang Province of China (QC2017020), Heilongjiang Bayi Agricultural University Support Program for San Heng San Zong (ZRCQC201904) and the Postdoctoral Science Foundation of Heilongjiang Bayi Agricultural University. The authors thank the reviewers, whose comments and suggestions helped us to improve this manuscript.
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Liu, Q., Shen, Y. & Yin, K. The antimicrobial activity of protein elicitor AMEP412 against Streptomyces scabiei. World J Microbiol Biotechnol 36, 18 (2020). https://doi.org/10.1007/s11274-019-2794-7
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DOI: https://doi.org/10.1007/s11274-019-2794-7