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Identification and characterization of Populus microRNAs in response to plant growth-promoting endophytic Streptomyces sp. SSD49

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Abstract

Endophytic Streptomyces sp. SSD49 inhibited eight pathogens, including the human opportunistic pathogenic microorganisms, the plant pathogenic fungi and bacteria. The growth of soybeans, tomatoes, peppers and Populus tomentosa seedings inoculated with SSD49 are remarkably promoted. Here, we constructed two P. tomentosa seedling microRNA (miRNA) libraries inoculated with (PS30d) and without SSD49 (PC30d) to explore the molecular regulatory roles in the plant response to the beneficial bacteria. Totals of 314 known and 144 novel miRNAs were identified, among which 27 known and 11 novel miRNA had significantly different expression. The targets of up-regulated miR160, miR156, ptc114 and down-regulated miR319 and other differential expressed miRNAs primarily regulated genes encoding transcription factors (auxin response factor, small auxin-up RNA, and GRAS proteins), disease resistance proteins, phytohormone oxidase, and response regulators, which could promote plant growth, influence disease resistance and miRNA biosynthesis in P. tomentosa. This is the first report on the genome-wide identification of biocontrol endophytic Streptomyces inoculation-responsive miRNAs using small RNA sequencing in P. tomentosa and these findings provide new insight into understanding the biocontrol effects of endophytic Streptomyces.

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Acknowledgements

This research was supported by the Fundamental Research Funds for the Central Universities (2017ZY14, 2016JX03), the National Science and Technology Ministry (2012BAC09B03), the National Natural Science Foundation of China (J1310005) and the Beijing Nova Program (2011033). We thank Prof. Wei He for the contribution of D. gregaria, B. dothidea CFCC 7926/7897 and L. quercina subsp. populi; Prof. Shidong Li for C. parasitica, S. sclerotiorum and P. capsici; and Prof. Deqiang Zhang for P. tomentosa tissue culture seedlings. We thank the professional Scientific and Technical Editing Service (https://www.textcheck.com/text/page/about) for the edition of the final version of the manuscript.

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Authors and Affiliations

  1. Department of Microbiology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China

    Wenjia Tian, Youyou Ge, Xiaoyu Liu, Guiming Dou & Yuchao Ma

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  1. Wenjia Tian
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  2. Youyou Ge
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  3. Xiaoyu Liu
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  4. Guiming Dou
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  5. Yuchao Ma
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YM conceived and designed the experiments. YG, XL and GD conducted experiments and analyzed data. WT and YG wrote the manuscript and should be as the first co-authors. All authors read and approved the manuscript.

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Correspondence to Yuchao Ma.

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Tian, W., Ge, Y., Liu, X. et al. Identification and characterization of Populus microRNAs in response to plant growth-promoting endophytic Streptomyces sp. SSD49. World J Microbiol Biotechnol 35, 97 (2019). https://doi.org/10.1007/s11274-019-2671-4

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