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Enzymatic gene expression by Pleurotus tuoliensis (Bailinggu): differential regulation under low temperature induction conditions

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Abstract

Pleurotus tuoliensis is a valuable, rare and edible mushroom that is been commercially cultivated and is rapidly developing in China markets. Low temperatures are required to induces primordia initiation for the successful production of fruiting bodies (basidiomes) during commercial cultivation. In this work, we investigated the enzymatic activities and performed transcription profiling analysis of enzymatic genes under different low temperature conditions. The results suggest that the enzymatic activities and transcription levels decrease or increase significantly at 4 and 13 °C. Lacc10 and mnp6 seems to play a dominant role during nutrition growth. Furthermore, the expression of laccase and peroxidase genes was highly correlated to the detected extracellular enzymatic activity. Cold stress genes expression profiles were upregulated under 4 °C/13 °C (3 days), while only the Hsp70 gene was downregulated (at the stage of fruiting bodies production) at 13 °C (12 days). Our results showed that the transcriptional regulation of laccase and ligninolytic peroxidase genes plays an important role in the fruiting bodies of Bailinggu under low temperature induction (4 °C). Induction at low temperatures was a highly important cultivation condition in Bailinggu.

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Data Availability

All data generated and analysed during this study are included in this published article. All model transcripts sequences were available in the JGI (https://jgi.doe.gov/).

Abbreviations

POD:

Peroxidase

PPO:

Polyphenol oxidase

CAT:

Catalase

SOD:

Superoxide dismutase

FC:

Fold change

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Acknowledgements

We would like to thank Dr. Lucía Ramírez from Centro de Investigaciones Biológicas (Spain) for his friendly work on the English editing and modification.

Funding

This study was supported by the National Natural Science Foundation of China (31471926).

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Authors and Affiliations

  1. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, College of Agriculture, Jilin Agricultural University, Changchun, 130118, China

    Shuang Hua, Bo Zhang, Yongping Fu, Bao Qi, Yanshuang Li, Fenghua Tian & Yu Li

Authors
  1. Shuang Hua
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  2. Bo Zhang
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  3. Yongping Fu
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  4. Bao Qi
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  5. Yanshuang Li
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  6. Fenghua Tian
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  7. Yu Li
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Contributions

SH was responsible for all experiment work and performed the statistical analysis. YL participated in the design of the study. BZ, BQ, YPF, YSL and FHT collected most of the material and cultivated strains. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Yu Li.

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Hua, S., Zhang, B., Fu, Y. et al. Enzymatic gene expression by Pleurotus tuoliensis (Bailinggu): differential regulation under low temperature induction conditions. World J Microbiol Biotechnol 34, 160 (2018). https://doi.org/10.1007/s11274-018-2487-7

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  • DOI: https://doi.org/10.1007/s11274-018-2487-7

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