Abstract
Brucellae are intracellular bacterial pathogens that cause Brucellosis, bringing great economic burdens to developing countries. The pathogenic mechanisms of Brucella are still poorly understood. Earlier immune response plays an important role in the Brucella infection. Phosphoglyceromutase (PGM) and dihydrodipicolinate reductase (DapB) were cloned, expressed, purified, and their immunocompetence was analyzed. Cytokines were detected by murine macrophages (RAW 264.7) and splenocytes that stimulated with the two recombinant proteins. The immune responses were analyzed by ELISA from mice with the two recombinant proteins immunized. TNF-α, IL-6 and IL-8 were produced in stimulated RAW 264.7 cells and splenocytes. Th1-type cytokines, IFN-γ and IL-2, induced in RAW 264.7 cells and splenocytes were higher then Th2-type cytokines, IL-4 and IL-5. Th2-related immune response was induced in splenocytes obtained 35 days after mice immunized with the two proteins. The production of IgG1 was higher than IgG2a in immunized mice. Taken together, our results demonstrated that the two proteins could induce Th1 and Th2-type immune responses in vivo and in vitro.
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Acknowledgements
This work was supported by grants from the National Natural Science Foundation of China (31602080, 31502067, 31460650 and 31360610), the Foundation of the Technology Department of Henan Province (172102310335, 172102310443), the National key Research and Development Program of China (2017YFD0500304), the University Key Research Project of Henan Province (16A230013, 14B230017), the Key Cultivation Project of Shangqiu Normal University (50014101), and the Innovation Scientists and Technicians Troop Construction Projects of Henan Province (C20130058).
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Li, Z., Zhang, H., Zhang, J. et al. Brucella abortus phosphoglyceromutase and dihydrodipicolinate reductase induce Th1 and Th2-related immune responses. World J Microbiol Biotechnol 34, 22 (2018). https://doi.org/10.1007/s11274-017-2405-4
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DOI: https://doi.org/10.1007/s11274-017-2405-4