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Global protein expression profile response of planktonic Aeromonas hydrophila exposed to chlortetracycline

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Abstract

The antibiotics resistance phenomena of Aeromonas hydrophila has become serious economic and public health problems for the world aquaculture industry and human health care. In this study, to investigate the instinct antibiotics adaptive mechanism of this pathogen, iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) based quantitative proteomics technologies were performed to compare the differential expression of A. hydrophila in planktonic status in response to chlortetracycline (CTC) stress and then identified total 1552 proteins including 285 altered proteins with 90 increasing and 195 decreasing abundance proteins. The following bioinformatics analysis showed that many metabolic metabolism pathways such as carbon metabolism, pyruvate metabolism, and glycolysis/gluconeogenesis were trend to down-regulated whereas β-Lactam resistance, RNA degradation, and amino acids biosynthesis processes were more likely to increase in CTC stress. The related pyruvate metabolism and β-Lactam resistance processes in mRNA level were further measured using the q-PCR method. Thus, an understanding of the behaviors of A. hydrophila in response to CTC would be helpful to reveal the antibiotics adaptive mechanism and for the development of novel antibiotics therapy.

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Acknowledgements

This work was sponsored by grants from NSFC projects (Nos. 31200105, 31670129 and 31470238), the Fujian Agricultural and Forestry University Foundation for Distinguished Young Scholars (Nos. XJQ201201) and Fujian-Taiwan Joint Innovative Center for Germplasm Resources and cultivation of crop (FJ 2011 Program, No. 2015-75, China).

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Authors and Affiliations

  1. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 35002, People’s Republic of China

    Wanxin Li, Zujie Yao, Xiangyu Zhang, Fang Huang, Wenxiong Lin & Xiangmin Lin

  2. Key Laboratory of Crop Ecology and Molecular Physiology of Fujian Universities, Fujian Agriculture and Forestry University, Fuzhou, 35002, People’s Republic of China

    Wanxin Li, Zujie Yao, Xiangyu Zhang, Fang Huang, Wenxiong Lin & Xiangmin Lin

  3. Agroecological Institute, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People’s Republic of China

    Wenxiong Lin & Xiangmin Lin

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  1. Wanxin Li
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  2. Zujie Yao
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  3. Xiangyu Zhang
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  4. Fang Huang
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Correspondence to Wenxiong Lin or Xiangmin Lin.

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Li, W., Yao, Z., Zhang, X. et al. Global protein expression profile response of planktonic Aeromonas hydrophila exposed to chlortetracycline. World J Microbiol Biotechnol 33, 68 (2017). https://doi.org/10.1007/s11274-017-2204-y

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